Application of a specific-ion electrode to the determination of disulfide groups in proteins

Harrap, B. S.; Gruen, L.C.

doi:10.1016/0003-2697(71)90054-6

Cited by 17 publications

(6 citation statements)

References 23 publications

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“…Such studies have included the use of the solid state silver sulfide membrane electrode for both direct and indirect determination of organic and biological species (2)(3)(4). Thiols have been determined directly (5), as well as by titration (6)(7)(8) and the argentometric titration of proteins has been reported (9)(10)(11).…”

mentioning

confidence: 99%

Protein response of silver sulfide crystal membrane electrodes

D’Orazio¹,

Rechnitz²

1977

Anal. Chem.

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The direct response of AgzS crystal membrane electrodes to sulfur-containing proteins has been investigated using model compounds of varying disulfide and sulfhydryl content. Potentiometric measurements on solutions of the proteins lysozyme, ovalbumin, human serum albumin, and ribonuclease A show clear relationships between electrode response and protein conformation. Studies involving denaturation and refolding of model proteins demonstrate that electrode response is a function of the free sulfhydryl content of proteins and not of disulfide linkages. The mechanistic information obtained lays the groundwork for the analytical use of AgzS electrodes for the direct measurement of proteins in solution.Recently, ion selective electrodes have found increasing use

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confidence: 99%

Protein response of silver sulfide crystal membrane electrodes

D’Orazio¹,

Rechnitz²

1977

Anal. Chem.

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“…A Ag/S specific-ion electrode has been successfully used in determining sulfide [Bock andPuff, 1968 andNauman andWeber, 1971]; silver [Muller, et al, 1969 andFletcher andMannion, 1970]; H2S [Gruenwedel and Patnaik, 1971]; thiol [Gruen and Harrap, 1971] _ and disulfide [Harrap and Gruen, 1971]. The double junction Ag/AgCl electrode has been suggested for use as a reference electrode by Orion Research Inc. [1972].…”

Section: Review On Methodology Of Determining Taamentioning

confidence: 99%

“…The thiol amino acids were determined by the method of Harrap and Gruen [1971] from the hydrolyzates of FP1 and FP 3 -FP6. The results are presented in Table 17 along with corresponding results obtained by AAA method.…”

Section: Estimation Of Thiol Amino Acids By Ionalyzer Methodsmentioning

confidence: 99%

“…Therefore, it is necessary to find a suitable environment in which to conduct the experiment so that the -SH groups remain available to AgN0 3 titration. NaBH4 was suggested as an appropriate reductant for -SS-groups in proteins by Harrap and Gruen [1971]. Considering the above mentioned facts, the experiments were conducted in the following manner.…”

Section: A the Preparation Of Fpmentioning

confidence: 99%

“…Older analytical procedures for determining thiol are both cumbersome and lengthy. A relatively new procedure for the determination of thiol and disulfide groups in soluble proteins, utilizing a silver/sulfide (Ag/~ specific-ion electrode has been developed by Harrap and Gruen [1971].…”

Section: Introductionmentioning

confidence: 99%

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Thiol Amino Acids in Quality Control and Nutritive Value of Fishery Products

Ali¹

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Determination of thiol groups and disulphide bonds in wheat flour and dough

Graveland¹,

Bosveld²,

Marseille³

1978

J Sci Food Agric

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A study was made of the conditions for determining all thiol (SH) groups and disulphide (S-S) bonds in wheat flour and dough. Thiol groups were determined by amperometric titration with Ag2S04, the flour or dough which contains proteins and peptides being suspended in a solution of 6 M urea and 10-4 M sodium dodecyl sulphate (SDS). The potential of the platinum electrode was -0.24 V vs calomel reference electrode. Disulphide bonds were determined in two ways: (a) Using an excess of Ag2S04 in the presence of 9 M urea; when the reaction was finished, the excess A g~S 0 4 was removed by adding an exact quantity of glutathione. The glutathione which remained after binding the Ag2S04 excess was determined by amperometric titration with a 5.10-5 M A g~S 0 4 solution. (b) Determination after reduction with NaBH4; the disulphide bonds were reduced to thiol groups and the total amount of thiol groups was determined with Ag2S04. The results indicated that wheat flour contains 5-7 pmol SH-groups, which is about six times the level found by previous workers, and 11-18 pmol disulphide bonds per gram flour. A correlation with baking quality was not observed.

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Application of a specific-ion electrode to the determination of disulfide groups in proteins

Cited by 17 publications

References 23 publications

Protein response of silver sulfide crystal membrane electrodes

Protein response of silver sulfide crystal membrane electrodes

Thiol Amino Acids in Quality Control and Nutritive Value of Fishery Products

Determination of thiol groups and disulphide bonds in wheat flour and dough

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