1983
DOI: 10.1016/0009-8981(83)90232-2
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Application of a simple immunoadsorption assay for the measurement of saturated and unsaturated transcobalamin II and R-binders

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Cited by 25 publications
(12 citation statements)
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“…In our study, the central 95% reference interval for serum holoTC in 303 healthy adults and elderly was 37-171 pmol/L (geometric mean, 79 pmol/L). These values are consistent with the values obtained previously by methods that separate TC from haptocorrin (4,6 ) and also by a new method described by Nexø et al (13 ) in which apoproteins are first separated from holoproteins and then the TC fraction of the holoproteins is directly measured by ELISA.…”
Section: Discussionsupporting
confidence: 92%
See 1 more Smart Citation
“…In our study, the central 95% reference interval for serum holoTC in 303 healthy adults and elderly was 37-171 pmol/L (geometric mean, 79 pmol/L). These values are consistent with the values obtained previously by methods that separate TC from haptocorrin (4,6 ) and also by a new method described by Nexø et al (13 ) in which apoproteins are first separated from holoproteins and then the TC fraction of the holoproteins is directly measured by ELISA.…”
Section: Discussionsupporting
confidence: 92%
“…The majority of the methods described measure holoTC indirectly as the difference between total Cbl and the amount of haptocorrin-bound Cbl. Because the concentrations of these variables are high, a small holoTC value may not be precise (4,5,12 ). Only one in-house method that directly quantifies holoTC has been described previously (6 ), but it is too laborious and imprecise for routine use.…”
mentioning
confidence: 99%
“…Our reference interval compares well with previously published values obtained by methods using immunoprecipitation of HC and subsequent measurement of cobalamin or vitamin B 12 analogs bound to HC by assays that used HC as binding protein (8,9 ). The intraindividual variation of total HC (6%) and holoHC (6%) in our study was low compared with previously reported results for cobalamin (9%) and holoTC (16%) (12 ), which most likely reflects that holoHC is a more stable marker over time than is holoTC, as judged from its half-life of more than a week compared with the half-life of TC, which is in the magnitude of hours (2 ).…”
Section: Discussionsupporting
confidence: 84%
“…The present study measures TC I/HC with a RIA that quantifies total TC I/HC directly. The reference interval for this RIA was comparable to but slightly lower (20 ) than the intervals for two reported additive methods of determining total TC I/HC (21,22 ). As presaged in a preliminary abstract of part of the data (23 ), the findings reported here suggest that mild TC I/HC deficiency is relatively common and is responsible for many otherwise unexplained low cobalamin concentrations.…”
supporting
confidence: 58%
“…As discussed elsewhere (20 ), most assays of TC I/HC have measured its ability to bind cobalamin added in vitro, thus detecting only unsaturated apo-TC I/HC. However, cobalamin-saturated holo-TC I/HC usually exceeds apo-TC I/HC in plasma (21,22 ), which limits the informativeness of measurements of apo-TC I/HC alone. The present study measures TC I/HC with a RIA that quantifies total TC I/HC directly.…”
mentioning
confidence: 99%