Application of a Portable Infrared Instrument for Simultaneous Analysis of Sugars, Asparagine and Glutamine Levels in Raw Potato Tubers

Abstract

The level of reducing sugars and asparagine in raw potatoes is critical for potato breeders and the food industry for production of commonly consumed food products including potato chips and French fries. Our objective was to evaluate the use of a portable infrared instrument for the rapid quantitation of major sugars and amino acids in raw potato tubers using single-bounce attenuated total reflectance (ATR) and dial path accessories as an alternative to time-consuming chromatographic techniques. Samples representing a total of 84 experimental and commercial potato varieties harvested in two consecutive growing seasons (2012 and 2013) were used in this study. Samples had wide ranges of sugars determined by HPLC-RID (non-detectable (ND)-7.7 mg glucose, ND-9.4 mg fructose and 0.4-5.4 mg sucrose per 1 g fresh weight), and asparagine and glutamine levels determined by GC-FID (0.7-2.9 mg and 0.3-1.7 mg per 1 g fresh weight). Infrared spectra collected from 64 varieties were used to create partial least squares regression (PLSR) calibration models that predicted the sugar and amino acid levels in an independent set of 16 validation potato varieties. Excellent linear correlations between infrared predicted and reference values were obtained. PLSR models had a high correlation coefficient of prediction (rPred >0.95) and residual predictive deviation (RPD) values ranging between 3.1 and 5.5. Overall, the results indicated that the models could be used to simultaneously predict sugars, free asparagine and glutamine levels in the raw tubers, significantly benefiting potato breeding, certain aspects of crop management, crop production and research.

Quality Control of Honey Using New Generation Infrared Spectrometers

“…These devices can produce spectral quality equivalent to their benchtop counterparts and they provide the flexibility of being carried to factories, farmers and farms . These systems have been recently used for the determination of acrylamide in potato chips, quality of butters (Pujolras et al, 2015) and cornmeal (Ayvaz et al, 2015a), acrylamide precursors in raw potatoes (Ayvaz et al, 2015b), nutritional traits of potato breeding lines (Ayvaz et al, 2016a) and quality traits of tomato juice (Ayvaz et al, 2016b).…”

Prediction of glucose, fructose and sucrose content in cassava (Manihot esculenta Crantz) genotypes from Amazon using PLS models

“…Some authors obtained differences in glucose prediction parameters by the differential use of accessories in the spectrometer. Reporting SEP value between 0.46 and 0.38 mg/g for the use of ATR mode and dial-path respectively [22]. Thus, not only logical data processing is fundamental in a PLS analysis, but the proper use of accessories in the equipment as well.…”

Comparison of Selected Dimensionality Reduction Methods for Detection of Root-Knot Nematode Infestations in Potato Tubers Using Hyperspectral Imaging

“…Biotic and abiotic stressors can cause changes in spectral signatures, by triggering various defense mechanisms, such as production of specific metabolites, induction of hypersensitive reactions, and changes in plant tissues [ 7 ]. The most extensive use of remote sensing of potato tubers has been for quality assessment, e.g., detection of defects [ 13 ] and bruises [ 14 ], and chemometric analyses, such as sugar [ 15 ], cellulose and starch content [ 16 ]. On the other hand, only a handful of studies deal with pest and disease detection in potato tubers.…”