Application of a Poisson-gamma model to study the influence of gamete concentration on sperm-oocyte fusion in the zona-free hamster egg penetration test

Aitken, R. John; Elton, R. A.

doi:10.1530/jrf.0.0780733

Cited by 12 publications

(7 citation statements)

References 14 publications

(30 reference statements)

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“…It is interesting that the only hint of benefit from the hamster results was in those men with good motile sperm concentration measurements. Al-though this failed to reach statistical significance, the possible trend is in accordance with previous work which demonstrates that the hamster test will define male fertility in couples with unexplained infertility (Aitken et al, 1984a) and also in donors for an artificial insemination programme (Irving and Aitken, 1987).…”

Section: Discussionsupporting

confidence: 89%

“…The most likely reason for false negative results in some of the cases was low gamete density. It has been shown that the mathematical probability of egg penetration is substantially reduced if the product of the number of sperm and the number of eggs is less than 50 (Aitken and Elton, 1986). This means that large numbers of hamster eggs have to be used to ensure a valid test of sperm function from a man with severe oligozoospermia.…”

Section: Discussionmentioning

confidence: 99%

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Prognostic Significance of the Zona‐free Hamster Egg Test

Hargreave

Aitken

Elton

1988

British Journal of Urology

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Hamster tests were performed on sperm samples submitted by 111 men attending a fertility clinic. The method used involved exposure of the sperm to an ionophore to stimulate capacitation as this has been shown to give better egg penetration rates. The men were followed up for a total of 166 couple years and note was made of any pregnancies. At the time of their initial assessment most of the men had poor sperm measurements using conventional methods of semen analysis. The hamster test results gave no additional prognostic information to that already known from the duration of involuntary infertility reported by the couple at their first clinic visit and sperm motility and concentration determined by conventional semen analysis. It was concluded that the hamster test was not useful in this situation.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Prognostic Significance of the Zona‐free Hamster Egg Test

Hargreave

Aitken

Elton

1988

British Journal of Urology

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“…This was because the method appeared to be convenient and was highly predictive of fertility after artificial insemination (Irvine & Aitken, 1986), or in-vitro fertilization (Aitken et al, 1987), although it was less successful in predicting the outcome of natural intercourse in a group of oligozoospermic men (Hargreave et al, 1988). Furthermore, because this protocol achieves a high hamster egg penetration rate with normal fertile samples, the number of gametes (and hamsters) required to obtain statistically valid data is decreased (Aitken & Elton, 1986).…”

Section: Introductionmentioning

confidence: 99%

The effect of A23187 concentration and exposure time on the outcome of the hamster egg penetration test

Ford¹,

Rees²,

McLaughlin³

et al. 1991

Int J of Andrology

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We investigated the conditions required to enhance the performance of human sperm in the hamster egg penetration test with the free acid form of A23187. The best performance was observed after stimulation with 2 microM A23187 for 1 h when the median penetration rate with sperm from fertile donors was 100% of eggs with 5.8 decondensed sperm heads/egg. Extending the stimulation period with 2 microM A23187 to 2 or 3 h, resulted in a progressive decrease in the penetration rate. In the absence of A23187, the penetration rate was lower (0.7 decondensed sperm heads/egg after 1 h) but increased with stimulation time. A similar picture was observed with sperm from patients taken for an IVF programme. For a pool of cryopreserved semen, the coefficient of variation of the penetration rate after stimulation with 2 microM A23187 for 1 h, expressed as decondensed sperm heads/egg, was 11% within and 20% between assays. There was no correlation between the outcome of the hamster egg penetration test and the percentage motility, velocity or lateral head displacement of the sperm measured after the same stimulation regime. However, in IVF patients the initial velocity and lateral head displacement of the sperm (zero time) were correlated with the best result from the hamster egg penetration test (r = 0.62 and 0.57 respectively). No motility changes characteristic of capacitation were detected. We conclude that stimulation with 2 microM A23187 (free acid) for 1 h prior to the addition of the zona free hamster eggs can produce a high penetration rate with fertile samples and provides a convenient and robust protocol for the assay. However, when carried out in this way the test does not assess the ability of the sperm to capacitate.

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“…A pool of control semen was created by mixing a number of ejaculates from fertile donors and cryopreserving it, and a sample from the pool was included in the test every day a hamster oocyte assay was run. The results of preliminary analyses showed no increase in predictive power after penetration rates had been adjusted to a constant concentration of motile spermatozoa (Aitken & Elton, 1986) and the results shown below are uncorrected.…”

Section: Semen Preparation and The Hamster Oocyte Penetration Testmentioning

confidence: 83%

Value of the hamster oocyte test and computerised measurements of sperm motility in predicting if four or more viable embryos will be obtained in an IVF cycle

et al. 2001

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The experimental group consisted of men from 81 couples waiting for in vitro fertilization (IVF), about half of whom had sperm dysfunction defined by a negative post-coital test. A diagnostic semen sample was subjected to a hamster oocyte penetration test (HOPT) after stimulation of the acrosome reaction with A23187 +/- pentoxifylline and to computerized sperm motility measurements (CASA) as well as conventional semen analysis according to the WHO protocol. Logistic regression was used to identify parameters that predicted the probability of achieving four or more viable embryos at IVF among the 65 couples from whom four or more oocytes were collected. The number of oocytes available and whether the woman had previously been pregnant (ever pregnant) were important factors but once these had been taken into account a number of sperm parameters had additional predictive power. The most useful of these were the percentage sperm static (CASA) or the percent sperm progressively motile (conventional semen analysis) in the Percoll preparation. A model incorporating the number of oocytes collected, ever pregnant and percentage sperm static achieved 85% correct prediction of outcome in the experimental dataset but only 62% correct prediction in an independent set of 280 IVF cycles. The percentage of hamster oocytes penetrated was a significant predictor but had no advantage over simple motility measurements. The results illustrate the difficulty of basing a prognosis for achieving satisfactory fertilization in IVF on the properties of spermatozoa.

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Application of a Poisson-gamma model to study the influence of gamete concentration on sperm-oocyte fusion in the zona-free hamster egg penetration test

Cited by 12 publications

References 14 publications

Prognostic Significance of the Zona‐free Hamster Egg Test

Prognostic Significance of the Zona‐free Hamster Egg Test

The effect of A23187 concentration and exposure time on the outcome of the hamster egg penetration test

Value of the hamster oocyte test and computerised measurements of sperm motility in predicting if four or more viable embryos will be obtained in an IVF cycle

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