Application of a photosystem II model for analysis of fluorescence induction curves in the 100 ns to 10 s time domain after excitation with a saturating light pulse

PS II model-based simulations of single turnover flash-induced transients of fluorescence yield monitored within the time domain of 100 ns–10 s on dark-adapted Chlorella pyrenoidosa cells

“…1 provides in a more comprehensive manner than the previous version (Belyaeva et al 2006) a suitable basis for analyzing SFITFY curves monitored in the unicellular green alga Chlorella Pyrenoidosa Chick. As a result of these investigations, it is important to note that a reasonable data fit can only be achieved when contributions due to effects of nonradiative recombination in PS II and transient 3 Car formation as nonphotochemical quencher are properly taken into account (Schödel et al 1998(Schödel et al , 1999Steffen 2003;Steffen et al 2005a, b).…”

“…To describe the primary processes of photosynthesis, we developed a generalized reaction scheme (Riznichenko et al 1999;Lebedeva et al 2000Lebedeva et al , 2002Belyaeva et al 2003;Kamali et al 2004;Belyaeva et al 2006) that includes the following processes: light-induced electron transport coupled to the transmembrane proton transfer, DW generation and its effect on the transmembrane charge transfer, K ? and Cl -ion transport, ATPsynthase activity, and the role of buffer groups in lumen and stroma.…”

On the approaches applied in formulation of a kinetic model of photosystem II: Different approaches lead to different simulations of the chlorophyll a fluorescence transients

“…Lazár et al, 1997;Tomek et al, 2001) or more complicated (e.g. Baake and Schlöder, 1992;Stirbet et al, 1998;Vredenberg et al, 2001;Lazár, 2003;Zhu et al, 2005;Belyaeva et al, 2006), but also complex models were considered describing, in addition to PSII, also subsequent electron transport reactions (e.g. Lebedeva et al, 2002;Kroon and Thoms, 2006;Laisk et al, 2006).…”

Analysis of initial chlorophyll fluorescence induction kinetics in chloroplasts in terms of rate constants of donor side quenching release and electron trapping in photosystem II

“…5 and 6), a sigmoidal rise of the MTF-induced fluorescence response curve. The sigmoidicity arises from the fact that the variable fluorescence Fv is controlled by the rate constant of light excitation k L and of release of donor side quenching k s 1 : Characteristics of donor side quenching can be read from the rise and decay kinetics of quenching release in ultra short single turnover excitations (Butler 1972;Mauzerall 1972;Steffen 2003;Steffen et al 2005;Belyaeva et al 2006). The 'degree' of sigmoidicity of F(t) is determined by the ratio between k L and k s 1 (Figs.…”

“…This increase has been ascribed to the release of photochemical quenching by Q A due to its reduction to Q A - (Duysens and Sweers 1963). The kinetics of the ns-STF-induced variable fluorescence in intact cells and chloroplasts have shown a biphasic rise in the 0.1-100 ls and a multiphasic recovery in the 0.05-10 4 ms time range, respectively (Mauzerall 1972;Steffen et al 2005;Belyaeva et al 2006). The kinetic pattern of the recovery phase is similar to that of STF-and TTF-induced responses (Nedbal et al 1999;Vredenberg et al 2006; with a major fast phase in the 0.05-1 ms time range ascribed to quenching recovery in association with Q A -reoxidation in Q B reducing RCs.…”