Application of a permethrin immunosorbent assay method to residential soil and dust samples

Chuang, Jane C.; Emon, Jeanette M. Van; Tefft, M; Wilson, Nancy K.

doi:10.1080/03601234.2010.493479

Cited by 11 publications

(9 citation statements)

References 19 publications

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“…[14, 22–24] Enzyme-linked immunosorbent assays (ELISA) have been presented as a cost effective alternative to instrumental methods. [25] Because of the potential health implications, it is important to monitor populations in order to form strategies to minimize exposure. Thus, we opted to determine both plasma and urinary 3-PBA in populations consisting of consumers and farmers in Thailand utilizing an immunoassay to monitor 3-PBA in urine [26] that had been further adapted and validated to measure 3-PBA in plasma following hydrolysis of adducts.…”

Section: Introductionmentioning

confidence: 99%

Determination of the pyrethroid insecticide metabolite 3-PBA in plasma and urine samples from farmer and consumer groups in northern Thailand

Thiphom

Prapamontol

Chantara

et al. 2013

Journal of Environmental Science and Health, Part B

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In this study, the enzyme-linked immunosorbent assays (ELISA) were modified to detect 3-PBA in plasma (including the adducted form) and urine among a large group of consumers and farmers in an agricultural area. The samples were collected on the same day in the morning from 100 consumers (50 females, 50 males) and 100 farmers (50 females, 50 males) in the Fang district, Chiang Mai province, northern Thailand. The ELISA was very sensitive having an IC50 value of 26.7 and 15.3 ng/mL, a limit of quantitation of 5 and 2.5 ng/mL and a limit of detection of 1.08 and 1.94 ng/mL for plasma and urine, respectively. These methods had low (< 5%) intra- and inter-assay coefficients of variation. The extraction technique satisfactorily eliminated the matrix effect from samples before ELISA analysis, yielding good recoveries (85.9–99.4% and 87.3–98.0%, respectively). For the volunteer study, the detection rate for plasma 3-PBA was 24% in consumers and 42% in farmers, but the median and range values were similar (median 5.87 ng/mL, range 5.16–8.44 ng/mL in consumers and 6.27 ng/mL, range 4.29–9.57 ng/mL in farmers). The rate of detection in the urine was similar (76% and 69%, in consumers and in farmers), yet the median concentration was significantly higher in farmers (8.86 μg/g creatinine in consumers vs 16.1 μg/g creatinine in farmers) and the range also much wider in farmers (1.62–80.5 μg/g creatinine in consumers and 0.80–256.2 μg/g creatinine in farmers). There was no correlation between plasma 3-PBA and urinary 3-PBA concentrations in the study presumably because plasma 3-PBA is a measure of cumulative exposures while urinary 3-PBA reflects acute exposures. In addition, metabolism and excretion of pyrethroids varies by individual. Nevertheless, this study demonstrated that these volunteers were exposed to pyrethroids. To our knowledge, this is the first report that compared plasma 3-PBA and urinary 3-PBA in a large group of volunteers. The ELISA method provided higher sample throughput with lower cost as compared to the instrumental analysis.

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Section: Introductionmentioning

confidence: 99%

Determination of the pyrethroid insecticide metabolite 3-PBA in plasma and urine samples from farmer and consumer groups in northern Thailand

Thiphom

Prapamontol

Chantara

et al. 2013

Journal of Environmental Science and Health, Part B

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“…However, the validity of the ELISA has been previously demonstrated for other pyrethroids and their metabolites in urine (50,51) and cypermethrin detection in other complex matrices. (52,53) Nonetheless, validation of the ELISA method for cypermethrin in urine is lacking and, therefore, is a limitation of the current study.…”

Section: Discussionmentioning

confidence: 90%

A Pilot Study of Workplace Dermal Exposures to Cypermethrin at a Chemical Manufacturing Plant

Buckley

Geer

Connor

et al. 2011

Journal of Occupational and Environmental Hygiene

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Exposure during the manufacture of pesticides is of particular concern due to their toxicity and because little is known about worker exposure, since most studies have focused on end-use application within agriculture or buildings. Even though dermal exposure can be expected to dominate for pesticides, little is known about workplace dermal exposures or even appropriate methods for their assessment. The current study begins to address this gap by evaluating alternative methods for assessing dermal exposure at a chemical manufacturing plant. For this pilot study, eight workers were recruited from a U.S. plant that produced the pesticide cypermethrin. Exposure was evaluated using three approaches: (1) survey assessment (questionnaire), (2) biological monitoring, and (3) workplace environmental sampling including ancillary measurements of glove contamination (interior and exterior). In each case, cypermethrin was quantified by enzyme-linked immunosorbent assay (ELISA). Environmental measurements identified two potential pathways of cypermethrin exposure: glove and surface contamination. Workplace exposure was also indicated by urine levels (specific gravity adjusted) of the parent compound, which ranged from 35 to 253 μg/L (median of 121 μg/L) with no clear trend in levels from pre- to post-shift. An exploratory analysis intended to guide future studies revealed a positive predictive association (Spearman correlation, p ≤ 0.10) between post-shift urine concentrations and a subset of survey questions evaluating worker knowledge, attitudes, and perceptions (KAP) of workplace dermal hazards, i.e., personal protective equipment self-efficacy, and inverse associations with behavior belief and information belief scales. These findings are valuable in demonstrating a variety of dermal exposure methods (i.e., behavioral attributes, external contamination, and biomarker) showing feasibility and providing measurement ranges and preliminary associations to support future and more complete assessments. Although these pilot data are useful for supporting design and sample size considerations for larger exposure and health studies, there is a need for validation studies of the ELISA assay for quantification of cypermethrin and its metabolites in urine.

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“…Immunochemical, biomarker and bioavailability methods are within this category. Immunochemical methods, in particular, have been used to quantitate pesticides and their metabolites, dioxins, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls and microbial products in several samples, including soil, sediment, water, milk, beef, potatoes, urine and serum (Van Emon et al, 1986;Johnson and Van Emon, 1996;William et al, 1996;Dankwardt and Hock, 1997;Barcelo´et al, 1998;Van Emon, 2001, 2006Nichkova et al, 2002Nichkova et al, , 2004Brena et al, 2005;Watanabe et al, 2006;Chuang et al, 2008). These methods use selective antibody or antibodies to bind the specific chemical of interest.…”

Section: Quantitative Screening Measurement Methodsmentioning

confidence: 99%

“…School projects checking for pesticides in community water sources have also relied on immunoassay testing kits. Simple extraction procedures can be coupled with immunoassay detection to analyze other samples of interest, such as soil, dust and air (Chuang et al, 2008). ELISA methods can also be modified and used for monitoring biomarkers in urine and other biological samples.…”

Section: Quantitative Screening Measurement Methodsmentioning

confidence: 99%

An overview of measurement method tools available to communities for conducting exposure and cumulative risk assessments

Medina-Vera

Emon

Melnyk

et al. 2009

J Expo Sci Environ Epidemiol

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Community-based programs for assessing and mitigating environmental risks represent a challenge to participants because each brings a different level of understanding of the issues affecting the community. These programs often require the collaboration of several community sectors, such as community leaders, local governments and researchers. Once the primary concerns, community vulnerabilities and assets are identified, participants plan on how to address immediate actions, rank known risks, collect information to support decision making, set priorities and determine an evaluation process to assess the success of the actions taken. The evaluation process allows the community to develop new action plans based on the results obtained from earlier actions. Tracking the success of the community actions may be as simple as a visual/tangible result (e.g., cleaning a park) or as complex as the collection of specific measurements to track the reduction of toxic pollutants or to determine the presence of a specific contaminant. Recognizing that communities may need to perform measurements to meet their goals, this paper provides an overview of the available measurement methods for several chemicals and biologicals in relevant environmental samples to a community setting. The measurement methods are organized into several categories according to their level of complexity, estimated cost and sources. Community project technical advisors are encouraged to examine the objective(s) of the community to be addressed by a measurement collection effort and the level of confidence that needed for the data to make appropriate decisions. The tables provide a starting point for determining which measurement method may be appropriate for specific community needs.

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Application of a permethrin immunosorbent assay method to residential soil and dust samples

Cited by 11 publications

References 19 publications

Determination of the pyrethroid insecticide metabolite 3-PBA in plasma and urine samples from farmer and consumer groups in northern Thailand

Determination of the pyrethroid insecticide metabolite 3-PBA in plasma and urine samples from farmer and consumer groups in northern Thailand

A Pilot Study of Workplace Dermal Exposures to Cypermethrin at a Chemical Manufacturing Plant

An overview of measurement method tools available to communities for conducting exposure and cumulative risk assessments

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