Application of a PCR-based test for detection of potato late blight and pink rot in tubers

Tooley, Paul W.; Carras, Marie M.; Lambert, D. H.

doi:10.1007/bf02853572

Cited by 22 publications

(17 citation statements)

References 14 publications

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“…Histograms with the same letter do not differ significantly according to Newman-Keuls test (P < 0.05). The bars represent the standard error of the means of five replicates (Tooley et al, 1998). Potato storage decay is suspected to be due partly to in-storage travel of pathogens including P. infestans within tuber tissues and from tuber to tuber.…”

Section: Discussionmentioning

confidence: 99%

“…1: Tooley et al 1998;2: White et al 1990; 3: Kageyama et al 1997;4: Liu et al, 1994;5: Zotobowska and Pospieszny, 1998;6: Mishra, Fox, & Culham, 2003;7: Nazar, Hu, Schmidt, Culham, & Robb, 1991;8: Robb, Hu, Platt, & Nazar, 1994 approaches were taken into account. In the static approach, data were reported in centimeters away from the inoculation site, whereas in the dynamic approach, progress in detection related to the distance from the front of the expanding lesion ahead of which the pathogen was detected by PCR.…”

Section: Pathogensmentioning

confidence: 99%

“…For each pathogen, PCR was performed using pathogen specific primers (de Boer & Ward, 1995;Kageyama, Ohyama, & Hyakumachi, 1997;Liu, Chatteljee, & Chattedee, 1994;Tooley et al, 1998;White, Bruns, Lee, & Taylor, 1990;Zotobowska & Pospieszny, 1998) (Table 1). All the primers were first checked using DNA samples extracted from pathogens grown on culture media.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

“…None of the integrated management programmes currently used completely eliminate pathogens, which persist and propagate during storage, leading to serious postharvest losses. Tooley, Carras, and Lambert (1998) have reported that even when tubers are clean in appearance at harvest, they may still harbour one or more pathogens that can spread quickly under conducive storage conditions, thus decreasing income. Therefore, heavy use of chemical pesticides is necessary to maintain reasonable yield during the growing season (Platt & Mahuku, 2000).…”

Section: Introductionmentioning

confidence: 98%

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PCR-based determination of colonization patterns during potato tuber infection by single and multiple pathogens

et al. 2007

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Potato tubers piled in storage are prone to infection by numerous pathogens. Each pathogen can cause damage alone, but severe losses often arise when more than one pathogen is involved. Currently, only a visual diagnosis is practiced on potato tubers before storing them, which does not allow any prediction of further disease spread. The aim of the present study was to determine differences in patterns of tissue colonization by several tuber decay pathogens and how late blight infection affects further tuber colonization by other important tuber pathogens. This study was conducted using artificial inoculation of potato tubers and PCR to provide an early and accurate diagnosis of disease development for major potato tuber rots, and to assess potential synergism/antagonism between Phytophthora infestans and other pathogens in stored tubers. In order to accurately follow the progress of each pathogen in tuber tissues, samples were collected over time from both the surface (peel, 0-2 mm depth) and internal tissues (flesh, depth > 2 mm) of the tubers at various distances from the inoculation site, at 3, 5, 7, 10, 12, 14, 17, and 19 days after inoculation. Successful detection of single or multiple pathogens was achieved using specific PCR-primers for each pathogen. Pathogens were always detected several centimeters ahead of the visible lesions. This tracking enabled us to determine the extent of colonization both on the tuber's surface and in internal tissues by each tested pathogen, either after single or multiple infections involving P. infestans as the primary pathogen. The presence of P. infestans was shown to enhance the development of Pectobacterium atrosepticum and to slow down that of P. erythrospetica and Pythium ultimum. No noticeable effect on further tuber colonization by F. sambucinum, V. dahliae or V. albo-atrum was observed in the presence of P. infestans. This approach involving more than one pathogen is more realistic than classical studies considering single pathogens, and may be helpful in monitoring the sanitary status of stored tubers. Our results make the outcome of certain combinations of pathogens in potato tubers more predictable and may result in more efficient preventive measures.

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Section: Discussionmentioning

confidence: 99%

Section: Pathogensmentioning

confidence: 99%

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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PCR-based determination of colonization patterns during potato tuber infection by single and multiple pathogens

et al. 2007

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“…After two transfers of hyphal tips on media containing antibiotics, twelve pure isolates were obtained ( Table 1). The identity of isolates of P. infestans was confirmed by polymerase chain reaction (PCR) using species-specific primers (Tooley, 1998) and their morphological traits according to Erwin and Ribeiro (2005). The isolates were kept on potato dextrose agar (PDA) at 5 o C in the Culture Collection of the Institute of Pesticides and Environmental Protection, Belgrade.…”

Section: Samples Collection and Isolationmentioning

confidence: 99%

Sensitivity of Phytophthora infestans (Mont.) de Bary isolates to fluazinam, fosetyl-Al and propamocarb-hydrochloride

et al. 2011

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SUMMARYA survey of in vitro sensitivity of twelve isolates of the Phytophthora infestans to the fluazinam, fosetyl-Al and propamocarb-hydrochloride was conducted. The isolates were isolated from infeceted potato leaves collected from eight different localities in Serbia during [2005][2006][2007]. All P. infestans isolates were sensitive to tested fungicides. The obtained values of resistance factor were in the range from 1.0 to 2.8. The EC 50 values of fluazinam were from 0.14 to 0.27 mg l -1 , fosetyl-Al from 30.2 to 85.8 mg l -1 , propamocarb-hydrochloride between 12.1 and 31.1 mg l -1 , respectively.

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Detection and Identification of Postharvest Microbial Pathogens

Narayanasamy

2005

Postharvest Pathogens and Disease Management

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Application of a PCR-based test for detection of potato late blight and pink rot in tubers

Cited by 22 publications

References 14 publications

PCR-based determination of colonization patterns during potato tuber infection by single and multiple pathogens

PCR-based determination of colonization patterns during potato tuber infection by single and multiple pathogens

Sensitivity of Phytophthora infestans (Mont.) de Bary isolates to fluazinam, fosetyl-Al and propamocarb-hydrochloride

Detection and Identification of Postharvest Microbial Pathogens

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