Application of a novel phosphinothricin N-acetyltransferase (RePAT) gene in developing glufosinate-resistant rice

Abstract

Currently, only few glufosinate-resistant genes are available for commercial application. Thus, developing novel glufosinate-resistant genes with commercial feasibility is extremely important and urgent for agricultural production. In this study, we transferred a newly isolated RePAT gene into a japonica rice variety Zhonghua11, resulting in a large number of independent T0 transgenic plants, most of which grew normally under high-concentration glufosinate treatment. Four transgenic plants with one intact RePAT expression cassette integrated into the intergenic region were selected. Agronomic performances of their T2 progenies were investigated, and the results suggested that the expression of RePAT had no adverse effect on the agronomic performance. Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of RePAT by assay on the medium and qRT-PCR. The inheritance and expression of RePAT in two transgenic plants were confirmed to be stable. Finally, the two-year field assay of glufosinate resistance suggested that the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha). Collectively, our study proves the high resistance of a novel gene RePAT to glufosinate and provides a glufosiante-resistant rice variety with agricultural application potential.

Seleção de sementes de soja geneticamente modificadas com o gene marcador bar por meio do teste de germinação em solução de glufosinato de amônio

“…Resultados similares com níveis variados de resistência ao glufosinato também foram observados em arroz. Cui et al (2016) registraram que 73% das plantas positivas para o gene bar cresceram normalmente sem clorose ou descoloração, indicando que o gene conferiu resistência ao glufosinato de amônio às plantas GMs. Segundo Carbonari et al (2016), a presença do gene bar, usado como marcador, garante um bom nível de resistência ao herbicida, embora esteja abaixo do nível de resistência de cultivares transformados geneticamente exclusivamente para este propósito, como as comercialmente liberadas.…”

Comparative genome analysis of the vineyard weed endophyte Pseudomonas viridiflava CDRTc14 showing selective herbicidal activity

“…5 and Table 3 ). The gene was used to develop glufosinate resistant transgenic rice 40 . In vitro testing confirmed (Fig.…”

The Development of Herbicide Resistance Crop Plants Using CRISPR/Cas9-Mediated Gene Editing

“…The CP4 gene, glyphosate N-acetyltransferase (GAT) gene, and glyphosate oxidoreductase (GOX) gene were the primary genes that contributed to glyphosate resistance in crops [37,38]. Transgenic glufosinate-resistant crops with the phosphinothricin N-acetyltransferase (PAT) gene or bialaphos resistance (Bar) gene have also been illegally commercialized [39][40][41]. Crops resistant to other herbicide types, such as ALS-inhibiting herbicides, ACCase-inhibiting herbicides, synthetic auxin herbicides, and HPPD-inhibiting herbicides, can also be developed by this method.…”