Application of a novel phage LPCS28 for biological control of Cronobacter sakazakii in milk and reconstituted powdered infant formula

Zou, Geng; Ndayishimiye, Libère; Xin, Lingxiang; Cai, Manshan; Zhang, Longjian; Liu, Jie; Song, Zhiyong; Wu, Ruiyi; Zhou, Yang; Shi, Yuanguo; Ye, Yingwang; Zhou, Rui; Li, Jinquan

doi:10.1016/j.foodres.2023.113214

Cited by 5 publications

(1 citation statement)

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“…[ 46 , 47 , 48 ], P. lactis [ 49 ], S. aureus [ 50 ], Cronobacter spp. [ 51 , 52 ], Y. enterocolitica [ 53 ], and others. Numerous studies have conclusively demonstrated that the application of bacteriophages and phage cocktails serves as a promising biocontrol strategy to safeguard dairy products, particularly milk, from bacterial contamination within the dairy industry.…”

Section: Discussionmentioning

confidence: 99%

A Genomic Analysis of the Bacillus Bacteriophage Kirovirus kirovense Kirov and Its Ability to Preserve Milk

Kazantseva

Skorynina

Piligrimova

et al. 2023

IJMS

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Bacteriophages are widely recognized as alternatives to traditional antibiotics commonly used in the treatment of bacterial infection diseases and in the food industry, as phages offer a potential solution in combating multidrug-resistant bacterial pathogens. In this study, we describe a novel bacteriophage, Kirovirus kirovense Kirov, which infects members of the Bacillus cereus group. Kirovirus kirovense Kirov is a broad-host-range phage belonging to the Caudoviricetes class. Its chromosome is a linear 165,667 bp double-stranded DNA molecule that contains two short, direct terminal repeats, each 284 bp long. According to bioinformatics predictions, the genomic DNA contains 275 protein-coding genes and 5 tRNA genes. A comparative genomic analysis suggests that Kirovirus kirovense Kirov is a novel species within the Kirovirus genus, belonging to the Andregratiavirinae subfamily. Kirovirus kirovense Kirov demonstrates the ability to preserve and decontaminate B. cereus from cow milk when present in milk at a concentration of 104 PFU/mL. After 4 h of incubation with the phage, the bacterial titer drops from 105 to less than 102 CFU/mL.

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