Application of a novel exposure limit approach for co-exposure of ‎chemicals: a field study by in-vitro design

Shekarloo, Masoomeh Vahabi; Panjali, Zahra; Mehrifar, Younes; Ramezanifar, Soleiman; Naziri, Seyed Husein; Koozekonan, Aysa Ghasemi; Moradpour, Zahra; Zendehdel, Rezvan

doi:10.1080/09603123.2022.2084513

Cited by 2 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Owing to the constraints associated with the absence of observable detrimental effects at any dose level (NOAEL), the utilization of the lower con dence interval of the benchmark dose (BMDL) as an OEL level has gained traction in recent years due to its superior advantages over NOAEL (14).…”

Section: Introductionmentioning

confidence: 99%

Benchmark dose determining airborne crystalline silica particles based on A549 lung-cell line survival in an in vitro study

Rafieepour,

Shekarloo,

Ashtarinezhad

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Crystalline silica has emerged as a prominent occupational toxicant over extended periods, leading to the development of lung fibrosis and cancer. The objective of this investigation is to establish a benchmark dose (BMD) for crystalline silica micro and nanoparticles based on the viability of the A549 lung-cell line. The impact of exposure to crystalline silica micro-particles (C-SiO2 MPs) and crystalline silica nanoparticles (C-SiO2 NPs) on A549 epithelial lung cells was examined for durations of 24 and 72 hours to evaluate cell viability using the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay. The determination of dose-response and BMD was carried out through the BMD software v 3.2. The findings reveal a dose-dependent relationship between cell viability and both C-SiO2 MPs and -NPs. The BMDL values for 24-h treatment of C-SiO2 MPs and -NPs were determined to be 2.26 and 0.97 µg/ml, respectively, based on exponential models. Correspondingly, these values were found to be 1.17 and 0.85 µg/ml for the 72-h treatment. This investigation underscores the significance of particle size as a contributing factor in assessing occupational health risks. Moreover, the utilization of BMDL can facilitate the determination of more precise values for occupational exposures by considering various parameters associated with particle presence.

show abstract

Section: Introductionmentioning

confidence: 99%