Application of a new IBD-based QTL mapping method to common wheat breeding population: analysis of kernel hardness and dough strength

Abstract

Mapping quantitative trait loci (QTL) in plants is usually conducted using a population derived from a cross between two inbred lines. The power of such QTL detection and the estimation of the effects highly depend on the choice of the two parental lines. Thus, the QTL found represent only a small part of the genetic architecture and can be of limited economical interest in marker-assisted selection. On the other hand, applied breeding programmes evaluate large numbers of progeny derived from multiple-related crosses for a wide range of agronomic traits. It is assumed that the development of statistical techniques to deal with pedigrees in existing plant populations would increase the relevance and cost effectiveness of QTL mapping in a breeding context. In this study, we applied a two-step IBD-based-variance component method to a real wheat breeding population, composed of 374 F6 lines derived from 80 different parents. Two bread wheat quality related traits were analysed by the method. Results obtained show very close agreement with major genes and QTL already known for those two traits. With this new QTL mapping strategy, inferences about QTL can be drawn across the breeding programme rather than being limited to the sample of progeny from a single cross and thus the use of the detected QTL in assisting breeding would be facilitated.

Interactions between Glu-1 and Glu-3 loci and associations of selected molecular markers with quality traits in winter wheat (Triticum aestivum L.) DH lines

“…One of these QTLs with the largest effect was on chromosome 1D at the locus Glu-D1 . These results are consistent with several other studies, in which QTLs for dough strength were identified on chromosomes 1A, 1B, 1D, 2A, 2D, 5A, 5B, and 5D (Crepieux et al 2005; Kerfal et al 2010; Zanetti et al 2001). Moreover, Nelson et al (2006) found QTL for alveograph L at the locus Glu-A3 and QTL for alveograph W associated with the locus Gli-B1 .…”

Mapping quantitative trait loci (QTLs) associated with dough quality in a soft×hard bread wheat progeny

“…In addition, Groos et al (2004) reported eleven QTLs for DStren not only on chromosomes 1B, 2D, 5A, and 5B but also on 1 A, ID, 3A, 4A, 4D, 7D, and one QTL linked to gwml30. Also, Crepieux et al (2005) detected for dough strength, three significant QTL located on chromosomes 1A, IB, and ID, close to the HMWglutenin loci, in homoeologous position. Furthermore, Nelson et al (2006) revealed that dough strength was consistently increased by alleles on IBS near the G/i-Bl gliadin loci.…”

“…In wheat, QTLs have been mapped for many quality traits such as grain protein content (Blanco et al, 2002;Campbell et al, 2001;Groos et al, 2003;Joppa et al, 1997;Sourdille et al, 2003;Suprayogui et al, 2009;Turner et al, 2004;Zanetti et al, 2001), grain hardness (Sourdille et al, 2003;Turner et al, 2004;Zanetti et al, 2001), and dough quality traits such as mixing time, mixing tolerance, dough tenacity and dough extensibility (Campbell et al, 2001;Cornish et al, 2001;Crepieux et al, 2005;Elangovan et al, 2008;Huang et al, 2006;Li et al, 2009;Ma et al, 2007;Mann et al, 2009;Nelson et al, 2006;Patil et al, 2009;Perretant et al, 2000;Sourdille et al, 2003;Zanetti et al, 2001).…”

Mixed linear model approaches in mapping QTLs with epistatic effects by a simulation study

“…Crepieux et al (2004) proposed an identity by descent QTL mapping method using plant breeding data for self-pollinated crops. Crepieux et al (2005) used this method to identify one QTL for kernel hardness and two QTLs for dough strength from data available in a wheat breeding program. The random model approach estimates a variance component associated with the QTL and identifies the marker interval that most likely contains the QTL.…”