Application of a modified minicolumn to detection of aflatoxins in corn

Arim, Rosario H.; Aguinaldo, Aida R.; Yoshizawa, Takumi

doi:10.2520/myco1975.1999.53

Cited by 7 publications

(6 citation statements)

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“…Mycotoxin Analysis. For the analysis of aflatoxins, all corn samples were qualitatively screened for aflatoxins according to the minicolumn method described previously (14). The positive minicolumn response for samples containing 5 µg/kg total aflatoxins and above was determined by visualizing the column under long-wave UV light.…”

Section: Methodsmentioning

confidence: 99%

Aflatoxins and Fumonisins in Corn from the High-Incidence Area for Human Hepatocellular Carcinoma in Guangxi, China

Yoshizawa

Kawamura

et al. 2001

J. Agric. Food Chem.

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154

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A comparative study on the natural occurrence of aflatoxins and Fusarium toxins was conducted with corn samples from high- and low-incidence areas for human primary hepatocellular carcinoma (PHC) in Guangxi, China. In samples from the high-risk area, aflatoxin B(1) was the predominant toxin detected in terms of quantity and frequency, with its concentration ranging between 9 and 2496 microg/kg and an 85% incidence of contamination. Among the samples, 13 (76%) exceeded the Chinese regulation of 20 microg/kg for aflatoxin B(1) in corn and corn-based products intended for human consumption. Significant differences in aflatoxin B(1), B(2), and G(1) and total aflatoxin concentrations in corn between the areas were found (P < 0.05). The average daily intake of aflatoxin B(1) from corn in the high-risk area was 184.1 microg, and the probable daily intake is estimated to be 3.68 microg/kg of body weight/day, 3.20 times the TD(50) in rats. Corn samples from both areas were simultaneously contaminated with fumonisins B(1), B(2), and B(3). Aflatoxin B(1) may play an important role in the development of PHC in Guangxi.

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Section: Methodsmentioning

confidence: 99%

Aflatoxins and Fumonisins in Corn from the High-Incidence Area for Human Hepatocellular Carcinoma in Guangxi, China

Yoshizawa

Kawamura

et al. 2001

J. Agric. Food Chem.

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154

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“…Solid-phase extraction (SPE) has been used in cleanup procedures saving a lot of time, being solvent-efficient and economical [7,37]. The use of SPE cartridges is simple and usually involves three steps: loading the sample, washing the impurities and eluting the toxin to be determined [13].…”

Section: -Introduçãomentioning

confidence: 99%

“…The use of SPE cartridges is simple and usually involves three steps: loading the sample, washing the impurities and eluting the toxin to be determined [13]. A variety of solid phase have been used in aflatoxin analysis, such as silicagel, surfacedmodified bonded silica like florisil and octadecil, a mixture of reversed-phase, ion exclusion, and ion exchange adsorbents (ISOLUTE and ROMER multifunctional column) and other minicolumns [1,3,6,7,25,27].…”

Section: -Introduçãomentioning

confidence: 99%

Determining aflatoxins B1, B2, G1 and G2 in maize using florisil clean up with thin layer chromatography and visual and densitometric quantification

Castro¹,

Vargas²

2001

Ciênc. Tecnol. Aliment.

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1 Recebido para publicação em 25/01/00. Aceito para publicação em 07/05/01. 2 ministério da agricultura e do abastecimento -laboratório de controle de qualidade e segurança alimentar/LAV/MG. Av. Raja Gabaglia, 245, BH, MG, Brasil, CEP 30380-090. email:gena@cdlnet.com.br; * A quem a correspondência deve ser enviada. SUMMARYA method for determining aflatoxins B 1 (AFB 1 ), B 2 (AFB 2 ),G 1 (AFG 1 ) andG 2 (AFG 2 ) in maize with florisil clean up was optimised aiming at one-dimensional thin layer chromatography (TLC) analysis with visual and densitometric quantification. Aflatoxins were extracted with chloroform: water (30:1, v/v), purified through florisil cartridges, separated on TLC plate, detected and quantified by visual and densitometric analysis. The in-house method performance characteristics were determined by using spiked, naturally contaminated maize samples, and certified reference material. The mean recoveries for aflatoxins were 94.2, 81.9, 93.5 and 97.3% in the range of 1.0 to 242 µg/kg for AFB 1 , 0.3 to 85mg/kg for AFB 2 , 0.6 to 148mg/kg for AFG 1 and 0.6 to 140mg/kg for AFG 2 , respectively. The correlation values between visual and densitometric analysis for spiked samples were higher than 0.99 for AFB 1 , AFB 2 , AFG 1 and 0.98 for AFG 2 . The mean relative standard deviations (RSD) for spiked samples were 16.2, 20.6, 12.8 and 16.9% for AFB 1 , AFB 2 , AFG 1 and AFG 2 , respectively. The RSD of the method for naturally contaminated sample (n = 5) was 16.8% for AFB 1 and 27.2% for AFB 2 . The limits of detection of the method (LD) were 0.2, 0.1, 0.1 and 0.1mg/kg and the limits of quantification (LQ) were 1.0, 0.3, 0.6 and 0.6mg/kg for AFB 1 , AFB 2 , AFG 1 and AFG 2 , respectively. (218 words) Keywords: aflatoxins; florisil; maize; thin-layer chromatography (TLC); densitometry. RESUMODETERMINAÇÃO DE AFLATOXINAS B 1 , B 2 ,G 1 EG 2 EM MI-LHO UTILIZANDO PURIFICAÇÃO COM FLORISIL, SEPARA-ÇÃO POR CROMATOGRAFIA EM CAMADA DELGADA E QUANTIFICAÇÃO VISUAL E DENSITOMÉTRICA. Um méto-do para determinação de aflatoxinas B 1 (AFB 1 ), B 2 (AFB 2 ),G 1 (AFG 1 ) eG 2 (AFG 2 ) em milho utilizando florisil na etapa de purificação foi otimizado com vistas a cromatografia em camada delgada (CCD) unidimensional com quantificação visual e densitométrica. As aflatoxinas foram extraídas com solução de clorofórmio: água (30:1, v/v), purificada em cartuchos de florisil, separada em placas cromatográfica de sílicagel, detectadas e quantificadas por análise visual e densitométrica. As características do método foram determinadas utilizando amostras de milho natural e artificialmente contaminadas e material de referência certificado. Os valores de recuperação média para cada aflatoxina obtidos na faixa de 1,0 a 242 µg/kg para AFB 1 , 0,3 a 85mg/kg para AFB 2 , 0,6 a 148 mg/kg para AFG 1 e 0,6 a 140mg/ kg para AFG 2 foram respectivamente 94,2; 81,9 93,5 e 97,3% por análise densitométrica. As correlações obtidas entre análi-se visual e densitométrica para amostras artificialmente contaminadas foram maiores que 0,99 para AFB 1 ...

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“…Mycotoxin contamination in the agricultural products of the country happens during the pre-and post-harvest stages of production (27). However, research in the Philippines is more focused on aflatoxin contamination in food and feeds because of its prevalence and the serious threat to human and animal health (29,30). According to Arim et al (29), more than 50% of corn stocks in government and privately owned warehouses were estimated to be contaminated with non-tolerable aflatoxin concentrations ( > 20 μg/kg).…”

Section: Introductionmentioning

confidence: 99%

Assessment of mycotoxin risk on corn in the Philippines under current and future climate change conditions

Salvacion¹,

Pangga²,

Cumagun³

2015

Reviews on Environmental Health

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AbstractThis study attempts to assess the risk of mycotoxins (aflatoxins and fumonisins) contamination on corn in the Philippines under current and projected climate change conditions using fuzzy logic methodology based on the published range of temperature and rainfall conditions that favor mycotoxin development. Based on the analysis, projected climatic change will reduce the risk of aflatoxin contamination in the country due to increased rainfall. In the case of fumonisin contamination, most parts of the country are at a very high risk both under current conditions and the projected climate change conditions.

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Application of a modified minicolumn to detection of aflatoxins in corn

Abstract: A simple, rapid and economical minicolumn packed with "lahar" (a local adsorbent material) and Florisil, previously developed for aflatoxins in copra meal, was adopted for corn. at the detection limit of 5 ƒÊg/kg of aflatoxin.

Cited by 7 publications

References 1 publication

Aflatoxins and Fumonisins in Corn from the High-Incidence Area for Human Hepatocellular Carcinoma in Guangxi, China

Aflatoxins and Fumonisins in Corn from the High-Incidence Area for Human Hepatocellular Carcinoma in Guangxi, China

Determining aflatoxins B1, B2, G1 and G2 in maize using florisil clean up with thin layer chromatography and visual and densitometric quantification

Assessment of mycotoxin risk on corn in the Philippines under current and future climate change conditions

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