Application of a modified enzyme-linked immunosorbent assay for 3-amino-2-oxazolidinone residue in aquatic animals

Yu, Liu; Peng, Dapeng; Huang, Lingli; Wang, Yulian; Chang, Chao; Ihsan, Awais; Tao, Yanfei; Yang, Bo; Zhang, Yuan

doi:10.1016/j.aca.2010.02.010

Cited by 18 publications

(22 citation statements)

References 21 publications

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“…After a 9 week withdrawal period, AOZ concentrations from this study were in close agreement with those of Hoogenboom et al, McCracken et al, and Cooper et al, following 4, 7, and 6 week withdrawal periods, respectively (8,17,18). Similar depletion of AOZ was also reported in carp (24), Nile tilapia (27), channel catfish (28), shrimp (29), and eel (30).…”

Section: Discussionsupporting

confidence: 93%

Tissue Depletion and Concentration Correlations between Edible Tissues and Biological Fluids of 3-Amino-2-oxazolidinone in Pigs Fed with a Furazolidone-Medicated Feed

Huang

Wang

et al. 2010

J. Agric. Food Chem.

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Furazolidone has been prohibited for use in food animal production worldwide for its carcinogenicity and mutagenicity, but it is still illegally used in some farms because of its effectiveness and cheap price. Because of the food safety and economical concerns, it is necessary to find an efficient and low-cost way to monitor the misuse of furazolidone in food-producing animals. For this regard, the tissue depletion and tissue-biological fluid concentration correlations of 3-amino-2-oxazolidinone (AOZ), which is the marker residue of furazolidone, were studied in pigs. Pigs were dosed with 400 mg/kg of furazolidone in feed for 7 days and were sacrificed at the withdrawal time of 0.5, 7, 21, 35, 56, and 63 days. Muscle, liver, kidney, urine, and plasma were collected to detect the AOZ by a simplified indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). Results showed that AOZ was widely distributed in pigs and eliminated slowly after the digestion of furazolidone. The half-lives of AOZ in the plasma, urine, liver, kidney, and muscle were 13.7, 14.7, 13.6, 13.6, and 15.0 days, respectively. Good correlations of the AOZ concentration were found between plasma and muscle, plasma and liver, urine and liver, and urine and kidney in the depletion period of 7-63 or 21-63 days, with correlation coefficients of more than 0.97 and p values less than 0.05. These correlations can provide a basis for a simple and economical way using plasma/urine to monitor the illegal use of furazolidone in pigs without slaughter.

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Section: Discussionsupporting

confidence: 93%

Tissue Depletion and Concentration Correlations between Edible Tissues and Biological Fluids of 3-Amino-2-oxazolidinone in Pigs Fed with a Furazolidone-Medicated Feed

Huang

Wang

et al. 2010

J. Agric. Food Chem.

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“…Thus, the concentration (IC 50 and detection limit) by ELISA was higher than the TR-FIA value. The IC 50 and detection limits in this study were lower than those in published studies (Vass et al 2005;Franek et al 2006;Chang et al 2008;Cheng et al 2009;Liu et al 2010;Zhu et al 2010;Jester et al 2014), indicating the high sensitivity of these methods.…”

Section: Sensitivity Of the Ic-elisa And Tr-fiacontrasting

confidence: 82%

“…In the recent years, enzyme-linked immunosorbent assays (ELISA) have been developed to determine AOZ in aquatic animals, chicken, pork, beef, and eggs (Cooper et al 2004;Diblikova et al 2005;Vass et al 2005;Franek et al 2006;Chang et al 2008;Cheng et al 2009;Liu et al 2010;Zhu et al 2010;Jester et al 2014). As an ultrasensitive and specific immunoassay technology, time-resolved fluoroimmunoassays (TR-FIA) are based on lanthanide chelates that have unique fluorescent properties, characterized by narrow-band emission lines, long Stokes shifts, high quantum yields, and long decay lifetimes (Le et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Determination of 3-Amino-2-oxazolidinone in Animal Tissue by an Enzyme-Linked Immunosorbent Assay and a Time-Resolved Fluoroimmunoassay

Yu²

2014

Analytical Letters

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An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a time-resolved fluoroimmunoassay (TR-FIA) for the determination of the furazolidone metabolite 3-amino-2-oxazolidinone (AOZ) were developed. 3-Amino-2-oxazolidinone was detected using its derivative, 3-[[(2-nitrophenyl)-methylene]-amino]-2-oxazolidinone (NPAOZ). 3-([(3-Carboxyphenyl)methylene]-amino)-2-oxazolidinone (CPAOZ) was used as the immunizing hapten for the production of monoclonal antibodies against NPAOZ. The ic-ELISA had a 50 percent inhibition (IC 50 ) value of 0.82 microgram per liter and a detection limit of 0.11 microgram per liter. The recoveries from animal tissues spiked with AOZ from 0.2 to 5 micrograms per kilogram were 82.3-112.8 percent with coefficients of variation values of 3.5-12.5 percent. The time-resolved fluoroimmunoassay (TR-FIA) showed a 50 percent inhibition of 0.56 microgram per liter and a detection limit of 0.07 microgram per liter. The recoveries and coefficients of variation were 87.2-117.4 percent and 3.6-8.5 percent, respectively. The analysis of fortified samples by both methods provided similar results to those obtained by a standard high-performance liquid chromatographytandem mass spectrometry method. These results indicate the ic-ELISA and TR-FIA methods are suitable for the determination of 3-amino-2-oxazolidinone in animal tissue.

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“…Franek et al (2006) developed an ELISA for NPAOZ in eggs with a CCβ of 0.3 µg/kg. For fish, a number of NPAOZ ELISAs have been developed with LOD/LOQ values of 0.1/0.3 µg/kg (Cheng et al, 2009;Tsai et al, 2009;Liu et al, 2010b). For pork, chicken and beef muscle and liver samples, ELISA tests for NPAOZ were developed with a CCβ value of 0.4 µg/kg (Diblikova et al, 2005), with LOD values of 0.3-0.4 µg/kg (Chang et al, 2008) and with an LOD value of 1.0 µg/kg (Nesterenko et al, 2012).…”

Section: Screening Methodsmentioning

confidence: 99%

Scientific Opinion on nitrofurans and their metabolites in food

Panel¹

2015

EFS2

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Nitrofurans are antimicrobial agents not authorised for use in food-producing animals in the European Union. Nitrofurans are rapidly metabolised, occurring in animal tissues as protein-bound metabolites. The European Commission requested EFSA to provide a scientific opinion on the risks to human health related to the presence of nitrofurans in food and whether a reference point for action (RPA) of 1.0 µg/kg for the marker metabolites is adequate to protect public health. Data on occurrence of nitrofuran marker metabolites in food were extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF). The CONTAM Panel concluded that these data were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which a single nitrofuran marker metabolite is present at 1.0 µg/kg in foods of animal origin, excluding milk and dairy products. The mean chronic dietary exposure for this worst-case scenario would range from 3.3 to 8.0 and 1.9 to 4.3 ng/kg b.w. per day for toddlers and adults, respectively. Nitrofurans and their marker metabolites, generally, are genotoxic and carcinogenic and, also, have non-neoplastic effects in animals. Margins of exposure (MOEs) were calculated at 2.0 × 10 5 or greater for carcinogenicity and at 2.5 × 10 3 or greater for non-neoplastic effects. The CONTAM Panel concluded that it is unlikely that exposure to food contaminated with nitrofuran marker metabolites at or below 1.0 µg/kg is a health concern. A scenario in which foods are considered to be contaminated with semicarbazide, from use of carrageenan as a food additive, at 1 µg/kg was used to assess whether it is appropriate to apply the RPA to foods of non-animal origin; MOEs of greater than 10 4 calculated for nonneoplastic effects do not indicate a health concern.© European Food Safety Authority, 2015 However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances that are high potency carcinogens, such as nitrofurans. As nitrofurans are excluded from that opinion, and taking into account that the presence of SEM in food may be from sources other than use of nitrofurazone, the European Commission (EC) requested the European Food Safety Authority (EFSA) for a scientific opinion on the risks to human health related to the presence of nitrofurans and their metabolites in food. The opinion should include (a) an evaluation of the toxicity of nitrofurans and their metabolites for humans, considering all relevant toxicological endpoints and identification of the toxicological relevance of nitrofurans and their metabolites present in food, and (b) an exposure assessment of the EU population to nitrofurans and their metabolites from food, including the consumption patterns of specific (vulnerable) groups of the population. In addition, the opinion should assess the appropriateness of...

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Application of a modified enzyme-linked immunosorbent assay for 3-amino-2-oxazolidinone residue in aquatic animals

Cited by 18 publications

References 21 publications

Tissue Depletion and Concentration Correlations between Edible Tissues and Biological Fluids of 3-Amino-2-oxazolidinone in Pigs Fed with a Furazolidone-Medicated Feed

Tissue Depletion and Concentration Correlations between Edible Tissues and Biological Fluids of 3-Amino-2-oxazolidinone in Pigs Fed with a Furazolidone-Medicated Feed

Determination of 3-Amino-2-oxazolidinone in Animal Tissue by an Enzyme-Linked Immunosorbent Assay and a Time-Resolved Fluoroimmunoassay

Scientific Opinion on nitrofurans and their metabolites in food

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