Application of a Method Incorporating Treatment with Daunomycin for the Selective Isolation of Slower-Growing Fungi

Sugimoto, Satoshi; Watanabe, Yoshinori; Isshiki, Kunio

doi:10.1271/bbb.80281

Cited by 3 publications

(5 citation statements)

References 12 publications

(11 reference statements)

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“…Selective isolation of slower-growing fungi on a medium containing the anthracycline antibiotic daunomycin could be a promising technique, which enhances the possibility for isolation of rare fungi from the environment (Sugimoto et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Myrmecridium hiemale sp. nov. from snow-covered alpine soil is the first eurypsychrophile in this genus of anamorphic fungi

Peintner

Knapp

Fleischer

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Myrmecridium hiemale sp. nov. was isolated from snow-covered alpine bare soil and is described as the first eurypsychrophilic species of this genus of filamentous fungi. Colony growth temperature experiments were carried out in the range 4-37 C. Morphological characteristics and colony appearance were in accordance with characteristics typical for Myrmecridium, but M. hiemale does not grow at temperatures of 25 C and above. Sequence analyses of the internal transcribed spacer and LSU rRNA D1/D2 regions indicated that the strain in question represents a distinct taxon within the genus Myrmecridium (Myrmecridiaceae, Sordariomycetes, Ascomycota). The type strain of M. hiemale is CBS 141017. A morphological description is provided, and a key is presented for the currently known taxa of Myrmecridium, a group of interesting fungi that are either saprobes or plant endophytes.

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Section: Discussionmentioning

confidence: 99%

Myrmecridium hiemale sp. nov. from snow-covered alpine soil is the first eurypsychrophile in this genus of anamorphic fungi

Peintner

Knapp

Fleischer

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…3 We isolated the producer of 1, Verticillium sp. f21794, from a soil sample via a new method 9 in which we applied the anthracycline antibiotic, daunomycin. The use of daunomycin in this method significantly prevents the growth of ordinary faster-growing fungi and, therefore, facilitates the selective isolation of rare slowergrowing fungi.…”

Section: Resultsmentioning

confidence: 99%

“…Verticillium sp. f21794 was isolated from a soil sample collected in Shirakurakyo, Shizuoka Prefecture, Japan, with the daunomycin method developed by Sugimoto et al The strain was cultured on a rotary shaker (220 rpm) at 25 °C for 4 days in a 500 mL Erlenmeyer flask containing 60 mL of a medium consisting of 2% potato starch, 1% glucose, 2% soybean meal, 0.1% KH 2 PO 4 , and 0.05% MgSO 4 ·7H 2 O.…”

Section: Methodsmentioning

confidence: 99%

JBIR-22, An Inhibitor for Protein−Protein Interaction of the Homodimer of Proteasome Assembly Factor 3

et al. 2010

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Proteasome assembling chaperone (PAC) 3 acts as a homodimer and plays an important role in proteasome formation. We screened JBIR-22 (1) as an inhibitor for protein-protein interaction (PPI) of PAC3 homodimer from our natural product library using a protein fragment complementation assay (PCA) with monomeric Kusabira-Green fluorescent protein (mKG) in vitro and found that 1 exhibited potent inhibitory activity against PAC3 homodimerization. Compound 1 showed long-term cytotoxicity against the human cervical carcinoma cell line, HeLa. This is the first report of a PPI inhibitor for proteasome assembly factors.

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“…Therefore, the screening efficiency is increased by the MAPC method. Also, the compounds discovered with this technique using whole-cell screening give new hope for discovering antibiotics from natural products (Lam, 2007;Suqimoto et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that the majority (99%) of microorganisms from the environment resist cultivation in the laboratory, but new techniques are rapidly improving cultivability (Kaeberlein et al 2002). Suqimoto et al (2008) established a new simple method using an anthracycline antibiotic that increased success in isolating rare funga genera. Zhu et al (2008) described a technique for isolating mycorrhizal fungi from roots of orchids that increased the isolation efficiency of previously uncultivable fungi.…”

Section: Discussionmentioning

confidence: 99%

A new screening method for discovering antibacterial agents from filamentous fungi

Chen¹,

Tan²,

Li³

et al. 2012

Afr. J. Biotechnol.

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Rapid development of resistance among major bacterial pathogens renders antibiotics more and more ineffective and it is crucial to find novel antibiotics for controlling these pathogens. Since highthroughput screening (HTS) that selects antibacterial agents according to targets in vitro rather than whole-cell have not proven effective in the discovery of new antibiotics; new approaches for discovering the next generation of antibiotics are urgently needed. Filamentous fungi are an important source for many of the antibiotics currently used, but screening them for novel antibiotics is difficult primarily due to the lack of efficient screening methods capable of maintaining whole bacterial cell metabolism. In this study, mixed agar plate culture (MAPC) screening method is described. The method maintains the advantages of traditional whole-cell screening but offers increased screening efficiency. Furthermore, its simplicity and convenience makes it suitable for many laboratories. MAPC screening increases the probability of discovering novel antibacterial agents from filamentous fungi under laboratory conditions.

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Application of a Method Incorporating Treatment with Daunomycin for the Selective Isolation of Slower-Growing Fungi

Cited by 3 publications

References 12 publications

Myrmecridium hiemale sp. nov. from snow-covered alpine soil is the first eurypsychrophile in this genus of anamorphic fungi

Myrmecridium hiemale sp. nov. from snow-covered alpine soil is the first eurypsychrophile in this genus of anamorphic fungi

JBIR-22, An Inhibitor for Protein−Protein Interaction of the Homodimer of Proteasome Assembly Factor 3

A new screening method for discovering antibacterial agents from filamentous fungi

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