Application of a Method for Estimating Day of Ovulation Using Urinary Estrogen and Progesterone Metabolites

Baird, Donna D.; McConnaughey, D. Robert; Weinberg, Clarice R.; Musey, Paul I.; Collins, Delwood C.; Kesner, James S.; Knecht, Edwin A.; Wilcox, Allen J.

doi:10.1097/00001648-199509000-00015

Cited by 123 publications

(78 citation statements)

References 5 publications

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“…This method is highly concordant with the use of midcycle surge of urinary LH and has comparable accuracy [28,29]. Its precision is similar to that of serum LH determination [30]. The method has recently been validated in a large sample of cycles using ultrasound evidence of follicular rupture [31].…”

Section: Hormone Assays and Hormonal Identification Of Ovulation And mentioning

confidence: 74%

Rescue of the Corpus Luteum in Human Pregnancy1

Baird

Weinberg

McConnaughey

et al. 2003

Biology of Reproduction

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Rescue of the corpus luteum from its programmed senescence maintains progesterone production required for pregnancy. In primates, chorionic gonadotropin produced by the developing conceptus acts as the primary luteotrophic signal. The purpose of this research was to assess corpus luteum rescue by examining changes in daily urinary progesterone metabolite levels during the first week after implantation. We determined the variability in progesterone metabolite profiles and evaluated its relationship to early pregnancy loss in 120 naturally conceived human pregnancies, including 43 early pregnancy losses. In other primates, an abrupt increase in the progesterone metabolite occurs at the time of implantation. This pattern occurred in an estimated 45% of the pregnancies in the present study. In the remaining pregnancies, there was a delayed rise (18%), neither a rise or decline (22%), or a decline (15%) during the week after implantation. The estimated rate of early pregnancy loss increased across these categories (from 5% loss with an abrupt rise at implantation to 100% loss with progesterone metabolite decline). Low urinary hCG levels in early pregnancy were significant determinants of a decline in postimplantation progesterone metabolite. However, preimplantation steroid metabolite levels were not significant, suggesting no inherent problem with the corpus luteum. Examination of individual progesterone metabolite profiles in relation to hCG profiles also indicated that few losses were caused by corpus luteum failure. Delineating the functional importance of an abrupt progesterone rise at the time of implantation may provide new strategies for promoting successful implantation in assisted reproduction. corpus luteum, corpus luteum function, human chorionic gonadotropin, pregnancy, progesterone

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Section: Hormone Assays and Hormonal Identification Of Ovulation And mentioning

confidence: 74%

Rescue of the Corpus Luteum in Human Pregnancy1

Baird

Weinberg

McConnaughey

et al. 2003

Biology of Reproduction

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“…The correlations between serum and urine measures in these EIAs compared favorably to those for widely used urinary EIAs (Table 6). Urinary PDG and E1C lagged behind serum by 1 day, on average, but they exhibited some slight pattern differences, with E1C lagging farther behind serum than PDG; these lag patterns need to be taken into consideration in algorithms that estimate day of ovulation (39 ).…”

Section: Discussionmentioning

confidence: 97%

Urinary Estrone Conjugate and Pregnanediol 3-Glucuronide Enzyme Immunoassays for Population Research

O’Connor

Brindle²,

Holman³

et al. 2003

Clinical Chemistry

100

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Background:Monitoring of reproductive steroid hormones at the population level requires frequent measurements, hormones or metabolites that remain stable under less than ideal collection and storage conditions, a long-term supply of antibodies, and assays useful for a range of populations. We developed enzyme immunoassays for urinary pregnanediol 3-glucuronide (PDG) and estrone conjugates (E1Cs) that meet these criteria. Methods: Enzyme immunoassays based on monoclonal antibodies were evaluated for specificity, detection limit, parallelism, recovery, and imprecision. Paired urine and serum specimens were analyzed throughout menstrual cycles of 30 US women. Assay application in different populations was examined with 23 US and 42 Bangladeshi specimens. Metabolite stability in urine was evaluated for 0 -8 days at room temperature and for 0 -10 freeze-thaw cycles. Results: Recoveries were 108% for the PDG assay and 105% for the E1C assay. Serially diluted specimens exhibited parallelism with calibration curves in both assays. Inter-and intraassay CVs were <11%. Urinary and serum concentrations were highly correlated: r ‫؍‬ 0.93 for E1C-estradiol; r ‫؍‬ 0.98 for PDG-progesterone.

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“…Premenopausal women, who had not taken any type of hormones, been pregnant, or breast-fed in the previous 6 months (n = 18,521), provided an initial 15-mL blood sample drawn on the third to fifth day of their menstrual cycle ( follicular blood draw) and a second 30-mL blood sample drawn 7 to 9 days before the anticipated start of their next cycle (luteal blood draw). We counted the timing of the luteal sample from the estimated first day of the woman's next menstrual cycle, as this generally is more accurate than counting forward from day 1 of the current cycle (22,23). Follicular samples were placed in a refrigerator for 8 to 24 hours by the participant; then, she aliquoted the plasma into a labeled cryotube included in the blood collection kit.…”

Section: Methodsmentioning

confidence: 99%

Association between Plasma Prolactin Concentrations and Risk of Breast Cancer among Predominately Premenopausal Women

2006

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Recent evidence suggests that prolactin may be positively associated with postmenopausal breast cancer risk; however, little data are available in younger women. Therefore, we conducted a prospective, nested case-control study to examine the relationship between plasma prolactin concentrations and breast cancer risk in predominately premenopausal women from the Nurses' Health Study II. Blood samples were collected from 1996 to 1999. The analysis includes 316 cases of breast cancer diagnosed after blood donation and before June 1, 2003, who had two controls matched on age, fasting status, time of day and month of blood collection, race/ethnicity, and timing of blood draw within the menstrual cycle. Sixty-three percent of participants provided a timed follicular and luteal menstrual phase blood sample; other women provided a single untimed sample. When including all women, we observed a positive association between prolactin and breast cancer risk [relative risk (RR), top quartile versus bottom quartile, 1.5; 95% confidence interval (95% CI), 1.0-2.3; P trend = 0.03] that was slightly stronger among estrogen receptorpositive/progesterone receptor-positive tumors (comparable RR, 1.9; 95% CI, 1.1-3.3; P trend = 0.04). Associations were similar among premenopausal women only. However, we did not find an association between prolactin and breast cancer risk among the subset of women who only provided timed samples (comparable RR, average of timed samples, 1.3; 95% CI, 0.8-2.3; P trend = 0.40). The association seemed stronger among women z45 years old and for cases diagnosed within f4 years of blood collection. Our data suggest a modest positive association between prolactin and breast cancer risk among predominately premenopausal women; however, further follow-up is needed to increase power for subgroup analyses. (Cancer Res 2006; 66(4): 2476-82)

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Application of a Method for Estimating Day of Ovulation Using Urinary Estrogen and Progesterone Metabolites

Cited by 123 publications

References 5 publications

Rescue of the Corpus Luteum in Human Pregnancy1

Rescue of the Corpus Luteum in Human Pregnancy1

Urinary Estrone Conjugate and Pregnanediol 3-Glucuronide Enzyme Immunoassays for Population Research

Association between Plasma Prolactin Concentrations and Risk of Breast Cancer among Predominately Premenopausal Women

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