Application of a Combined Transmittance/Fluorescence Leaf Clip Sensor for the Nondestructive Determination of Nitrogen Status in White Cabbage Plants

Kaniszewski, S.; Kowalski, Artur; Dyśko, J.; Agati, Giovanni

doi:10.3390/s21020482

Cited by 9 publications

(7 citation statements)

References 40 publications

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“…A similar strategy has been also applied in the set-up of a portable leaf clip-based device has been also set-up for the indirect, nondestructive assay of nitrogen in cabbage leaves, though the measurement of chlorophyll fluorescence. In fact, a correlation has been found to occur between the content of nitrogen in leaves and the ratio between the chlorophyll fluorescence signals measured under excitation at 650 nm and 365 nm, two wavelengths respectively unaffected or absorbed by flavonols and anthocyanins [42].…”

Section: Figurementioning

confidence: 99%

Light and Autofluorescence, Multitasking Features in Living Organisms

Croce

2021

Photochem

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Organisms belonging to all life kingdoms may have the natural capacity to fluoresce. Autofluorescence events depend on the presence of natural biomolecules, namely endogenous fluorophores, with suitable chemical properties in terms of conjugated double bonds, aromatic or more complex structures with oxidized and crosslinked bonds, ensuring an energy status able to permit electronic transitions matching with the energy of light in the UV-visible-near-IR spectral range. Emission of light from biological substrates has been reported since a long time, inspiring unceasing and countless studies. Early notes on autofluorescence of vegetables have been soon followed by attention to animals. Investigations on full living organisms from the wild environment have been driven prevalently by ecological and taxonomical purposes, while studies on cells, tissues and organs have been mainly promoted by diagnostic aims. Interest in autofluorescence is also growing as a sensing biomarker in food production and in more various industrial processes. The associated technological advances have supported investigations ranging from the pure photochemical characterization of specific endogenous fluorophores to their possible functional meanings and biological relevance, making fluorescence a valuable intrinsic biomarker for industrial and diagnostic applications, in a sort of real time, in situ biochemical analysis. This review aims to provide a wide-ranging report on the most investigated natural fluorescing biomolecules, from microorganisms to plants and animals of different taxonomic degrees, with their biological, environmental or biomedical issues relevant for the human health. Hence, some notes in the different sections dealing with different biological subject are also interlaced with human related issues. Light based events in biological subjects have inspired an almost countless literature, making it almost impossible to recall here all associated published works, forcing to apologize for the overlooked reports. This Review is thus proposed as an inspiring source for Readers, addressing them to additional literature for an expanded information on specific topics of more interest.

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Section: Figurementioning

confidence: 99%

Light and Autofluorescence, Multitasking Features in Living Organisms

Croce

2021

Photochem

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“…Ouzounis et al [63] confirmed the high correlation of the flavonol index determined with Dualex Scientific+ with the concentrations of flavonoids such as rutin and quercetin determined with the HPLC technique. Tremblay et al [64], Padilla et al [65], Agati et al [66], and Kaniszewski et al [67] confirmed the high correlation of the NBI index with the nutritional status of plants with respect to nitrogen.…”

Section: Chlorophyll Flavonol and Nitrogen Balance Indicesmentioning

confidence: 87%

“…It has also been shown that a high C/N ratio in plants stimulated the production of flavonoids, whereas a low C/N ratio inhibited their production [72]. In a study with cabbage [67], it was demonstrated that the chlorophyll index Our observations are generally consistent with the results of other authors relating to the various genotypes of lettuce, which indicate that blue light has a significant impact on the synthesis of bioactive compounds, including flavonoids [10][11][12]20,66,68]. In the case of red-leaf lettuce, supplementation of white light or red light with blue light has stimulated leaf pigmentation and the synthesis of secondary metabolites [27].…”

Section: Chlorophyll Flavonol and Nitrogen Balance Indicesmentioning

confidence: 99%

“…It has also been shown that a high C/N ratio in plants stimulated the production of flavonoids, whereas a low C/N ratio inhibited their production [72]. In a study with cabbage [67], it was demonstrated that the chlorophyll index and the nitrogen balance index (NBI) were positively correlated with the N content in the leaves, whereas the flavonol index was negatively correlated. Similar relationships were evident in our study.…”

Section: Chlorophyll Flavonol and Nitrogen Balance Indicesmentioning

confidence: 99%

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The Impact of LED Light Spectrum on the Growth, Morphological Traits, and Nutritional Status of ‘Elizium’ Romaine Lettuce Grown in an Indoor Controlled Environment

et al. 2021

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The study examined the influence of light quality on the growth and nutritional status of romaine lettuce grown in deep water culture with a floating raft system using two different nutrient solutions. Four spectra of LED light were used with different ratios of R, G, and B lights (80:10:10, 70:10:20, 60:10:30, and 70:18:12). Two nutrient solutions with a low (A) and moderately high (B) nutrient content were used. Regardless of the nutrient solution, the RGB 70:18:12 light promoted the production of leaf biomass as well as inhibited the accumulation of K and Mg in the leaves. Moreover, those plants were characterized by a low Nitrogen Balance Index (NBI) and a high flavonol index. In the last week of cultivation, there was a strong decrease in K, P, and nitrates in the nutrient solution, and an increase in Ca. In the final stage of growth, symptoms of withering of the tips of young leaves (tipburn) were observed on the plants. The most damage was observed on the plants growing under 70:10:20, 70:18:12, and with the higher concentration of minerals in the solution (B).

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“…The non-destructive detection of epidermal flavonols by the Dualex and Multiplex devices was also used to define an indicator of leaf nitrogen status, superior to those based on the detection of chlorophyll alone, as proximal sensing of the leaf nitrogen content on turfgrass 5 , cabbage crops 6,7 and ornamental plants 8 and proposed to drive precision fertilization.…”

Section: Chlorophyll Fluorescence Excitation Screening Methodsmentioning

confidence: 99%

In situ plant peel spectral attenuation of chlorophyll fluorescence excitation as a tool in precision agriculture and photobiology studies

Rossi,

Cavigli,

Tuccio

et al. 2024

Photonic Technologies in Plant and Agricultural Science

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Polyphenols are important compounds of the plant secondary metabolism, being involved in the plant response to abiotic and biotic stresses and conferring quality properties to fruits and vegetables. Their determination provides valuable information to be used in precision agriculture and in plant physiology studies. Non-destructive evaluation of polyphenols, present in leaf epidermises and fruit skins, can be determined indirectly by multispectral measurements of the chlorophyll fluorescence from the underlying cell layers. This technique, called the chlorophyll fluorescence excitation screening (ChlFES) method, is based on the spectral attenuation by superficial compounds of the incoming radiation impinging on chlorophyll molecules. Examples of the non-destructive monitoring of plant flavonoids response to different UV radiation regimes are reported. Application of the ChlFES to apples, olives, kiwifruits, plums and mainly wine grape to detect flavonoids and anthocyanins was aimed to select fruits with the highest nutraceutical value. In field appliance of the technique by portable fluorescence sensors allowed the control of the wine grape phenolic maturity during the season and the mapping of the grape phenolic content in the vineyard to perform selective harvest and then the production of top quality wines. Furthermore, the estimate of the plant nutrient status as proximal sensing of the leaf nitrogen content was proved on different crops and proposed to drive precision fertilization.

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Application of a Combined Transmittance/Fluorescence Leaf Clip Sensor for the Nondestructive Determination of Nitrogen Status in White Cabbage Plants

Cited by 9 publications

References 40 publications

Light and Autofluorescence, Multitasking Features in Living Organisms

Light and Autofluorescence, Multitasking Features in Living Organisms

The Impact of LED Light Spectrum on the Growth, Morphological Traits, and Nutritional Status of ‘Elizium’ Romaine Lettuce Grown in an Indoor Controlled Environment

In situ plant peel spectral attenuation of chlorophyll fluorescence excitation as a tool in precision agriculture and photobiology studies

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