Application of a combination of neutral red and amido black staining for rapid, reliable cytotoxicity testing of biomaterials

Ciapetti, G.; Granchi, Donatella; Verri, E.; Savarino, L.; Cavedagna, D.; Pizzoferrato, Alberto

doi:10.1016/s0142-9612(96)80001-9

Cited by 69 publications

(45 citation statements)

References 15 publications

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“…The latter categorization was chosen for this experiment where the test specimen was placed over confluent cells, as determined by some authors (16), with special emphasis on the changes that the material can cause to cells during the experiment. Other authors (17) have pointed out that direct contact of test specimens obtained from different materials may inhibit cell growth due to physical contact, not from the toxic substances that are released.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

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This study evaluated, in vitro, the cytotoxicity of six root canal sealers after 12, 24 and 72 h of contact time, using an endothelial ECV-304 cell line. The MTT assay was used for analysis of cell viability. Twelve specimens of each sealer were prepared and randomly assigned to 6 groups according to the commercial brands (n=4/time). A control group was also formed, which was not subjected to the contact with sealers. To assess the effects of sealers on endothelial cells, the specimens were placed in culture plate wells and incubated at 37°C with 5% CO 2 and 100% humidity. MTT assays were performed in quadruplicate after 12, 24 and 72 h of contact of the sealer specimens with monolayers. Statistical analysis was performed by two-way ANOVA with Bonferroni post-hoc test at a significance level of 5%. Analysis of absorbance in the experimental groups showed that GuttaFlow presented the lowest cytotoxicity, with a mean absorbance of 0.048, followed by Pulp Canal Sealer (0.038), Sealer 26 (0.038), Endo Densell (0.036) and Pulp Fill (0.035). The control group had a mean absorbance of 0.098. Based on the results, Endofill and GuttaFlow were the most and the least cytotoxic sealers, respectively.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

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“…The cytotoxicity of the membranes was tested by an in vitro cell viability method according to Ciapetti et al [11]. The test was carried out with dilution of the extracts of hydrogel membranes, in contact with mouse subconjunctive tissue cell culture, from NCTC Clone 929 line (ATCC-CCL1).…”

Section: Cytotoxicity Testsmentioning

confidence: 99%

“…The cytotoxicity of the hydrogels prepared by all methods was tested according to Ciapetti et al [11]. The relative count of total viable cells was correlated against hydrogel extracts.…”

Section: Cytotoxicity Testsmentioning

confidence: 99%

Direct UV photocrosslinking of poly(N-vinyl-2-pyrrolidone) (PVP) to produce hydrogels

Lopergolo

Lugão

Catalani

2003

Polymer

125

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Hydrogels for biomedical purposes, made from synthetic polymers as starting materials and free of co-adjuvant molecules, have been produced almost exclusively by high-energy radiative processes. On the other hand, UV photocrosslinking of such materials has been used in conjunction of monomers and/or photoinitiators. This work was addressed to the analysis of poly(N-vinyl-2-pyrrolidone) (PVP) submitted to direct photocrosslinking in aqueous solution, using low pressure Hg lamp (l em ¼ 254 nm). The process efficiency was evaluated, and the properties of the hydrogel formed were determined. The product thus formed has similar micro-and macroscopic properties, as compared to hydrogels produced by high-energy radiation and presents no cytotoxicity. These results demonstrated the viability of using this method as a versatile alternative to hydrogel production, broadening the possibility of its production where high-energy radiation facilities are not available. q

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“…Such work demonstrated that AZT has typical micromolar range efficacy against such cells, for example, {(DU 145 (prostate) IC 50 24 μM), (MCF-7 (breast) IC 50 22 μM), (CX-1 (colon) IC 50 23 μM)}. [9] Even when AZT is given at IC 10 or IC 20 , the drug can be effective because cell division is sufficiently slowed. When this level of AZT dosage is cycled, growth inhibition can become pronounced.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and In Vitro Assay of Folic Acid Conjugates of 3′-Azido-3′-Deoxythymidine (AZT): Toward Targeted AZT Based Anticancer Therapeutics

Vortherms

Doyle

Gao

et al. 2008

Nucleosides, Nucleotides and Nucleic Acids

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Conjugates of three components namely folic acid, poly(ethylene glycol) and 3′-azido-3′-deoxythymidine (AZT) are presented. Folate-PEG units were coupled to AZT to facilitate delivery of the nucleoside into the cell. A convenient separation of the polydisperse PEGylated-folic acid regioisomers produced upon conjugation is described. This is to select for the active γ-regioisomer over the inactive α-regioisomer. In vitro cytotoxicity assays were conducted against an ovarian cell line (A2780/AD) that overexpresses the folate receptor (FR) and compared to a FR free control cell line. Compared to AZT a ~ 20-fold greater potency against the resistant ovarian line was observed for the conjugates.

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Application of a combination of neutral red and amido black staining for rapid, reliable cytotoxicity testing of biomaterials

Cited by 69 publications

References 15 publications

Cytotoxicity of root canal sealers on endothelial cell cultures

Cytotoxicity of root canal sealers on endothelial cell cultures

Direct UV photocrosslinking of poly(N-vinyl-2-pyrrolidone) (PVP) to produce hydrogels

Synthesis, Characterization, and In Vitro Assay of Folic Acid Conjugates of 3′-Azido-3′-Deoxythymidine (AZT): Toward Targeted AZT Based Anticancer Therapeutics

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