Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples

Palát, Jiří; Kukučka, Petr; Codling, Garry; Price, Elliott J.; Janků, Petr; Klánová, Jana

doi:10.1016/j.chemosphere.2021.132300

Cited by 14 publications

(11 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, most NBFRs were not detected in the serum of mothers and children in a Pakistani population, except for BTBPE (mean concentration, 1.2 ng/g lw) . Similarly, in a Czech Republic study, relatively low concentrations of NBFRs were detected in the serum of 48 mothers . Conversely, the human serum levels of DBDPE (geomean, 40 ng/g lw) found in a brominated FR-producing region of Shandong were comparable with those reported here (geomeans for cases and controls, 50 and 34 ng/g lw, respectively).…”

Section: Resultssupporting

confidence: 75%

“…51 Similarly, in a Czech Republic study, relatively low concentrations of NBFRs were detected in the serum of 48 mothers. 52 Conversely, the human serum levels of DBDPE (geomean, 40 ng/g lw) found in a brominated FR-producing region of Shandong were comparable with those reported here (geomeans for cases and controls, 50 and 34 ng/g lw, respectively). However, these levels were much lower than those reported in e-waste recycling workers (125 ng/g lw).…”

Section: Serum Concentrations Of Targeted Frsmentioning

confidence: 99%

See 1 more Smart Citation

Exposure to Novel Brominated Flame Retardants and Organophosphate Esters and Associations with Thyroid Cancer Risk: A Case–Control Study in Eastern China

Liu

Meng

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

Novel brominated flame retardant (NBFR) and organophosphate ester (OPE) exposure may engender adverse effects on human health. However, present epidemiological information regarding the effects of such exposure is limited and controversial. In this case−control study, 481 serum samples were collected from patients with thyroid cancer (n = 242) and healthy controls (n = 239) in Shandong Province, eastern China. The levels of NBFRs and OPEs, thyroid hormones, and serum lipid parameters were measured in all the participants. Pentabromotoluene, 2,3-dibromopropyl 2,4,6 tribromophenyl ether, decabromodiphenylethane (DBDPE), tris (2-chloroethyl) phosphate (TCEP), and triphenyl phosphate (TPP) were widely detected (detection frequency > 60%) in all the participants. A significantly high risk association was found between exposure of NBFRs and OPEs (namely 1,2,3,4,5-pentabromobenzene, DBDPE, tri-n-propyl phosphate, tri[(2R)-1-chloro-2-propyl] phosphate, tris (1,3-dichloro-2-propyl) phosphate, and tris (2-butoxyethyl) phosphate) and thyroid cancer in both males and females. In the females of the control group, TCEP levels exhibited a significantly positive association with thyroid-stimulating hormone and a negative association with triiodothyronine (T3), free triiodothyronine (FT3), and free thyroxine (FT4) levels. Weighted quantile sum regression evaluated the mixed effects of the compounds on thyroid hormones levels and thyroid cancer. As a result, TPP accounted for the majority of the T3, thyroxine, and FT3 amounts. Our results suggest that NBFR and OPE exposure contributes to alterations in thyroid function, thereby increasing thyroid cancer risk.

show abstract

Section: Resultssupporting

confidence: 75%

Section: Serum Concentrations Of Targeted Frsmentioning

confidence: 99%

Exposure to Novel Brominated Flame Retardants and Organophosphate Esters and Associations with Thyroid Cancer Risk: A Case–Control Study in Eastern China

Liu

Meng

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“… 19 One important metabolomics study showed that plasma phospholipid removal (after protein precipitation) improves the nonlipid metabolite coverage due to decreased matrix effects. 20 Commercial phospholipid removal technologies show promise for analysis of environmental chemicals in human plasma or serum, and applications already include the targeted analysis of persistent organic pollutants by GC–MS, 21 and of per- and poly-fluoroalkyl substances (PFAS) by LC–MS. 22 Moreover, a chemical exposomics study by Chaker et al recommended their use, concluding that phospholipid removal provides complementary molecular coverage compared to protein-precipitation alone.…”

Section: Introductionmentioning

confidence: 99%

Phospholipid Removal for Enhanced Chemical Exposomics in Human Plasma

Sdougkou

Xie

Papazian

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

The challenge of chemical exposomics in human plasma is the 1000-fold concentration gap between endogenous substances and environmental pollutants. Phospholipids are the major endogenous small molecules in plasma, thus we validated a chemical exposomics protocol with an optimized phospholipid-removal step prior to targeted and non-targeted liquid chromatography high-resolution mass spectrometry. Increased injection volume with negligible matrix effect permitted sensitive multiclass targeted analysis of 77 priority analytes; median MLOQ = 0.05 ng/mL for 200 μL plasma. In non-targeted acquisition, mean total signal intensities of non-phospholipids were enhanced 6-fold in positive (max 28-fold) and 4-fold in negative mode (max 58-fold) compared to a control method without phospholipid removal. Moreover, 109 and 28% more non-phospholipid molecular features were detected by exposomics in positive and negative mode, respectively, allowing new substances to be annotated that were non-detectable without phospholipid removal. In individual adult plasma (100 μL, n = 34), 28 analytes were detected and quantified among 10 chemical classes, and quantitation of per- and polyfluoroalkyl substances (PFAS) was externally validated by independent targeted analysis. Retrospective discovery and semi-quantification of PFAS-precursors was demonstrated, and widespread fenuron exposure is reported in plasma for the first time. The new exposomics method is complementary to metabolomics protocols, relies on open science resources, and can be scaled to support large studies of the exposome.

show abstract

“…The most-used extraction methods for serum analysis are solid-phase extraction (SPE), liquid–liquid extraction (LLE), and, more recently, solid-phase microextraction (SPME) [ 3 , 6 , 21 , 27 ]. In recent years, QuEChERS (quick, easy, cheap, effective, rugged, and safe) based extraction methods have been developed and validated, as they allow for customization and optimization for specific purposes [ 9 , 16 , 17 , 18 , 28 ]. This type of extraction can handle the processing of large numbers of samples using a simple methodology, unlike SPE or LLE, which commonly require a larger volume of solvents that may be more harmful to the environment [ 10 , 21 ] or involve additional steps such as pH adjustment [ 1 , 11 , 13 , 14 ], making them more time-consuming and expensive [ 6 , 16 , 27 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples

Rial-Berriel,

Ramos-Luzardo,

Acosta-Dacal

et al. 2023

Toxics

View full text Add to dashboard Cite

Animals and humans are exposed to various residues that can have a detrimental impact on health, including carcinogenic potential, endocrine disruption, or fatal toxicity. The toxic burden can be evaluated in several biological samples, with serum being one of the preferred and most convenient options. In this study, we have applied and validated a method for detecting several hundred toxins in serum samples. This technique involved a single-step QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by analysis using gas and liquid chromatography coupled with mass spectrometry. With this methodology, we could detect and quantify up to 353 compounds, including persistent organic pollutants (POPs), pesticides, pharmaceuticals, and rodenticides, using just 250 µL of serum. Among them, 92% could be measured at concentrations below 1.25 ng/mL, making it ideal for biomonitoring. We applied this method to samples collected from camels (n = 40) and humans (n = 25). We detected naproxen, ketoprofen, paracetamol, levamisole, and some POPs in these samples. This study validated the ability to simultaneously detect a broad range of compounds in small volumes of serum.

show abstract

Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples

Cited by 14 publications

References 37 publications

Exposure to Novel Brominated Flame Retardants and Organophosphate Esters and Associations with Thyroid Cancer Risk: A Case–Control Study in Eastern China

Exposure to Novel Brominated Flame Retardants and Organophosphate Esters and Associations with Thyroid Cancer Risk: A Case–Control Study in Eastern China

Phospholipid Removal for Enhanced Chemical Exposomics in Human Plasma

Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples

Contact Info

Product

Resources

About