Application of 3D- printed hydrogels in wound healing and regenerative medicine

Deptuła, Milena; Zawrzykraj, Małgorzata; Sawicka, Justyna; Banach-Kopeć, Adrianna; Tylingo, Robert; Pikuła, Michał

doi:10.1016/j.biopha.2023.115416

Cited by 16 publications

(10 citation statements)

References 154 publications

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“…The potential applications of hydrogels are vast, as demonstrated by the numerous classifications available. Hydrogels can be classified based on their source (natural or synthetic), size (nano-, macro-, or bulk hydrogels), chain composition, ionic charge, method of crosslinking, response to various stimuli, or biodegradability [ 67 ]. Matrigel ® is one of the most well-known hydrogels in scientific research [ 68 ].…”

Section: 3d Cell Culturesmentioning

confidence: 99%

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Górnicki,

Lambrinow,

Golkar-Narenji

et al. 2024

Nanomaterials

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Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional cell cultivation methods. The most commonly used technique involves cultivating cells on a flat surface in a two-dimensional format due to its simplicity. A three-dimensional (3D) format can provide a microenvironment for surrounding cells. There are two main techniques for obtaining 3D structures based on the presence of scaffolding. Scaffold-free techniques consist of spheroid technologies. Meanwhile, scaffold techniques contain organoids and all constructs that use various types of scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels that are one of the most extensively studied forms of potential scaffolds for 3D culture up to 4D bioprinted biomaterials. 3D bioprinting is one of the most important techniques used to create biomimetic scaffolds. The versatility of this technique allows the use of many different types of inks, mainly hydrogels, as well as cells and inorganic substances. Increasing amounts of data provide evidence of vast potential of biomimetic scaffolds usage in tissue engineering and personalized medicine, with the main area of potential application being the regeneration of skin and musculoskeletal systems. Recent papers also indicate increasing amounts of in vivo tests of products based on biomimetic scaffolds, which further strengthen the importance of this branch of tissue engineering and emphasize the need for extensive research to provide safe for humansbiomimetic tissues and organs. In this review article, we provide a review of the recent advancements in the field of biomimetic scaffolds preceded by an overview of cell culture technologies that led to the development of biomimetic scaffold techniques as the most complex type of cell culture.

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Section: 3d Cell Culturesmentioning

confidence: 99%

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Górnicki,

Lambrinow,

Golkar-Narenji

et al. 2024

Nanomaterials

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“…Bioinks have a fundamental role in the successful fabrication of a skin tissue construct. Their physicochemical/mechanical properties and their composition need to be carefully selected in order to achieve high printability and high cell viability (Figure 8) and to assist cellular functions [135,137]. Bioinks need to be biocompatible to ease cell growth, exhibit controllable rheological properties (e.g., shear thinning) in order to flow effortlessly through the nozzle and possess enhanced shape fidelity after printing (i.e., redeem sufficient storage modulus after printing), biofunctionality to meet biomimicry requirements (i.e., they should mimic ECM regarding biochemical/biomechanical properties [136]) and mechanical stability [23,70,134,138,139], which can be difficult to accomplish with a single-component bioink [134].…”

Section: Three-dimensional Bioprintingmentioning

confidence: 99%

“…Three-dimensional bioprinting, a subfield of 3D printing, is an emergent adaptive bio-manufacturing technology for the accurate fabrication of complex topological constructs based on computer-aided design (CAD), which has been broadly applied to TE, modeling of organoids, etc. It involves the development of bioinks (i.e., biomaterial formulations that contain cells and bioactive agents such as growth factors and can be readily processed by an automated biofabrication technology [133][134][135]) to engineer biologically appropriate constructs mimicking and restoring natural ECM [136]. Bioinks have a fundamental role in the successful fabrication of a skin tissue construct.…”

Section: Three-dimensional Bioprintingmentioning

confidence: 99%

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Recent Developments in 3D-(Bio)printed Hydrogels as Wound Dressings

Kammona,

Tsanaktsidou,

Kiparissides

2024

Gels

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Wound healing is a physiological process occurring after the onset of a skin lesion aiming to reconstruct the dermal barrier between the external environment and the body. Depending on the nature and duration of the healing process, wounds are classified as acute (e.g., trauma, surgical wounds) and chronic (e.g., diabetic ulcers) wounds. The latter take several months to heal or do not heal (non-healing chronic wounds), are usually prone to microbial infection and represent an important source of morbidity since they affect millions of people worldwide. Typical wound treatments comprise surgical (e.g., debridement, skin grafts/flaps) and non-surgical (e.g., topical formulations, wound dressings) methods. Modern experimental approaches include among others three dimensional (3D)-(bio)printed wound dressings. The present paper reviews recently developed 3D (bio)printed hydrogels for wound healing applications, especially focusing on the results of their in vitro and in vivo assessment. The advanced hydrogel constructs were printed using different types of bioinks (e.g., natural and/or synthetic polymers and their mixtures with biological materials) and printing methods (e.g., extrusion, digital light processing, coaxial microfluidic bioprinting, etc.) and incorporated various bioactive agents (e.g., growth factors, antibiotics, antibacterial agents, nanoparticles, etc.) and/or cells (e.g., dermal fibroblasts, keratinocytes, mesenchymal stem cells, endothelial cells, etc.).

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“…These networks, capable of absorbing and retaining substantial volumes of water, are distinguished by their remarkable ability to swell without dissolution, maintaining structural integrity through chemical or physical cross-linking mechanisms [9,10]. This intrinsic property allows hydrogels to mimic the physicochemical aspects of the natural extracellular matrix, making them particularly suited for applications in drug delivery systems [7,9,[11][12][13][14][15][16][17][18][19], tissue engineering [20][21][22][23][24], wound healing [25][26][27][28], and beyond, as illustrated in Figure 1. The initiation of hydrogel research and its expansion into biomedical sciences exemplify a trajectory of innovation, highlighting the versatility of these materials in solving complex biological challenges and their role in advancing biomedical solutions.…”

Section: Introductionmentioning

confidence: 99%

Advances in Hydrogel-Based Drug Delivery Systems

Liu,

Chen

2024

Gels

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Hydrogels, with their distinctive three-dimensional networks of hydrophilic polymers, drive innovations across various biomedical applications. The ability of hydrogels to absorb and retain significant volumes of water, coupled with their structural integrity and responsiveness to environmental stimuli, renders them ideal for drug delivery, tissue engineering, and wound healing. This review delves into the classification of hydrogels based on cross-linking methods, providing insights into their synthesis, properties, and applications. We further discuss the recent advancements in hydrogel-based drug delivery systems, including oral, injectable, topical, and ocular approaches, highlighting their significance in enhancing therapeutic outcomes. Additionally, we address the challenges faced in the clinical translation of hydrogels and propose future directions for leveraging their potential in personalized medicine and regenerative healthcare solutions.

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Application of 3D- printed hydrogels in wound healing and regenerative medicine

Cited by 16 publications

References 154 publications

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Recent Developments in 3D-(Bio)printed Hydrogels as Wound Dressings

Advances in Hydrogel-Based Drug Delivery Systems

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