Application of 23 Full-Factorial Design for Development and Optimization of Biocompatible, Biodegradable Solid Lipid Nanoparticles Containing Curcumin

Abstract

Curcumin (C), a natural anticancer agent suffers extremely low aqueous solubility and rapid systemic elimination, therefore, the aim from the present study was to develop and optimize biocompatible, biodegradable solid lipid nanoparticles containing curcumin (CSLNs) using Solvent Injection Method. The components selected to develop the SLNs were Glycerol MonoStearate (GMS; the lipidic carrier), Poloxamer 407 (P407; Surfactant) and Ethanol (solvent for the drug and the lipidic carrier). The combination and ratios for the optimization process were carried out using 2 3 full-factorial designs. The experimental design runs (8 formulations H1-H8) were prepared and the design dependent responses (assessment of particle size, Entrapment Efficiency % and the in vitro release study) were characterized. The developed formulae showed a nanometric particle size range (203.0-345.0nm), high Entrapment Efficiency % (62.77-87.42 %) and prolonged release over 24 hr periods (55.97-89.64 %). These results were analyzed using JMP ® 10 software and its analytical tools were used to draw Pareto charts and the interactions plots. On the basis of the software analysis, formulation H9 with a desirability factor of 0.582 was selected as the optimized formulation and was evaluated for the dependent responses. Formulation H9 showed a particle size of 249.1 nm, 74.51 % Entrapment efficiency, 85.72 % in vitro release over 24 hr and these results suggested that the solid lipid nanoparticles formulations could be a promising method to sustain the release of curcumin.

Evaluation of Stability, Cellular Uptake and Anti-Tumour Activities of an Optimized Curcumin Solid Lipid Nanoparticles Formulation.

“…Curcumin analysis was preformed according to the methodology mentioned before (Dawaba et al, 2013). Briefly, curcumin was detected at 428 nm with a sample run time of 10 min and a flow rate of 1ml/min using WATERS ® HPLC system consisting of Perkin Elmer HPLC column (SPHERI-5 RP -18, Perkin Elmer LLC, Norwalk city, CT, USA) with the following specifications 5µm, 4.6mm×250mm Preceded by a guard column (SecurityGurad™, Phenomenex Inc., Torrance, CA, USA) filled with C18 cartridges (4 x 2.00 mm ID) at room temperature.…”

“…The optimized C-SLN formula was prepared according to the SIM protocols mentioned before (Dawaba et al, 2013). In brief, Curcumin (2% of the lipid phase) and the specified amount of GMS (148.79 mg) were dissolved in the specified volume of Ethanol (1ml) with gentle heating.…”

Solid Lipid Nanoparticles: Formulation, Preparation, and Characterization: A Review

“…[30] To prepare Curcumin SLNs, the solvent injection method is used which is simple, effective, and versatile and finally, the curcumin SLNs are obtained. [32] The complete procedure flow chart of solvent injection has depicted in Figure 10.…”

A Review on Lipid- Polymer Hybrid Nanoparticles and Preparation with Recent Update

“…Nanoparticles containing drug is normally prepared by using the standard method of high-pressure homogenization and precipitation from emulsions containing organic solvents. Nevertheless, preparation of nanoparticles with these methods face some challenges of high temperature, high pressure, toxicological solvents issues, high emulsifier concentration and so on [16]. Thus, the solvent injection approach exists as a new robust and versatile alternative method which are now generally utilised for the preparation of liposomes and polymeric nanoparticles.…”