Application note: A novel low-cost open-source LED system for microalgae cultivation

Wishkerman, Asher; Wishkerman, Ely

doi:10.1016/j.compag.2016.11.015

Cited by 41 publications

(25 citation statements)

References 28 publications

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“…As result, the photon conversion efficiency drops with increasing forward currents and maximal photon extraction cannot exceed a given threshold [78]. Arduino microcontroller coupled with standard LED luminaries (for examples see [50,84]). • Photo-and mixotrophy: Phototrophic organisms use light as energy source to fix inorganic carbon dioxide in organic compounds.…”

Section: Box -Technical Limits Of Flashing Light Sourcesmentioning

confidence: 99%

Flashing LEDs for Microalgal Production

Schulze

Guerra

Pereira

et al. 2017

Trends in Biotechnology

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Flashing lights are next-generation tools to mitigate light attenuation and increase the photosynthetic efficiency of microalgal cultivation systems illuminated by light-emitting diodes (LEDs). Optimal flashing light conditions depend on the reaction kinetics and properties of the linear electron transfer chain, energy dissipation, and storage mechanisms of a phototroph. In particular, extremely short and intense light flashes potentially mitigate light attenuation in photobioreactors without impairing photosynthesis. Intelligently controlling flashing light units and selecting electronic components can maximize light emission and energy efficiency. We discuss the biological, physical, and technical properties of flashing lights for algal production. We combine recent findings about photosynthetic pathways, self-shading in photobioreactors, and developments in solid-state technology towards the biotechnological application of LEDs to microalgal production.

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Section: Box -Technical Limits Of Flashing Light Sourcesmentioning

confidence: 99%

Flashing LEDs for Microalgal Production

Schulze

Guerra

Pereira

et al. 2017

Trends in Biotechnology

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“…The design can be modified to feature larger illuminator and orbital shaker to support larger culture volumes. The peak brightness of the illuminator can be changed by altering the spacing of the LED strips 1 . The spectral properties of the light source can be modified to suit different experimental regimes [25]. The white LED strips, described here, can be swapped for multi-color LED strips[15]. Multiple copies of the described LED controller can be built to independently control the output of the three colors of the multicolor LED strip, and thus vary the spectral properties and timing of the light source. The illumination light intensity and cycle can be controlled using a microcontroller or a computer for advanced growing protocols.…”

Section: Hardware Descriptionmentioning

confidence: 99%

Bottom-Illuminated Orbital Shaker for Microalgae Cultivation

Nedbal

Gao

Suhling

2020

Preprint

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A bottom-illuminated orbital shaker designed for the cultivation of microalgae suspensions is described in this open-source hardware report. The instrument agitates and illuminates microalgae suspensions grown inside flasks. It was optimized for low production cost, simplicity, low power consumption, design flexibility, consistent, and controllable growth light intensity.The illuminated orbital shaker is especially well suited for low-resource research laboratories and education. It is an alternative to commercial instruments for microalgae cultivation. It improves on home-built microalgae growth systems by offering consistent and well characterized illumination light intensity. The illuminated growth area is 20 cm × 15 cm, which is suitable for three T75 tissue culture flasks or six 100 ml Erlenmeyer flasks. The photosynthetic photon flux density, is variable in eight steps (26 − 800 µmol · m −2 · s −1 ) and programmable in a 24-hour light/dark cycle. The agitation speed is variable (0-210 RPM). The overall material cost is around £300, including an entry-level orbital shaker. The build takes about two days, requiring electronics and mechanical assembly capabilities. The instrument build is documented in a set of open-source protocols, design files, and source code. The design can be readily modified, scaled, and adapted for other orbital shakers and specific experimental requirements.The instrument function was validated by growing fresh-water microalgae Desmodesmus quadricauda and Chlorella vulgaris. The cultivation protocols, microalgae growth curves, and doubling times are included in this report. Hardware typeBiological sample handling and preparationOpen source license Creative Commons Attribution 4.0 International (CC BY 4.0) Cost of hardware £300Source file repository

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“…The authors reported innovations in data acquisition using sensors and storage of values in clouds, basing the study on the concept of internet of things (IoT). The authors proved that electronic technology has been fostering precision agriculture (Wishkerman and Wishkerman, 2017;Santos et al, 2018;Mazon-Olivo et al, 2018;Zhou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The increasing demand for efficient and accessible electronic systems has motivated research using prototyping platforms (Bajer and Krejkar, 2015;Mekki et al, 2015;Wishkerman and Wishkerman, 2017). Studies have used the Arduino platform to create agricultural control systems.…”

Section: Introductionmentioning

confidence: 99%

Development of an Electronic Controller for Lettuce Production in Greenhouses

Filho¹,

Rodrigues²,

Medeiros³

et al. 2020

Rev. Agric. Neotrop.

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The objective of this study was to develop an electronic controller for microclimate control in greenhouses, as well as to verify if the automated control system affects the productivity of two varieties of lettuce "Lactuca sativa". The control system was developed based on the Atmega 2560 and compatible transducers. An experimental field analysis was carried out over a production cycle for two lettuce varieties. The experimental results showed that the designed equipment worked according to the implemented programming algorithm. However, the ventilation, nebulization and shading actuators did not control environmental variables, due to under sizing. The irrigation process was correctly controlled throughout the experimental period. The electronic controller promoted increase in the productivity of lettuce varieties. There was increase of 28% in total fresh weight; 10% in stem diameter; 7 and 8% in height gain and average diameter, respectively.

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Application note: A novel low-cost open-source LED system for microalgae cultivation

Cited by 41 publications

References 28 publications

Flashing LEDs for Microalgal Production

Flashing LEDs for Microalgal Production

Bottom-Illuminated Orbital Shaker for Microalgae Cultivation

Development of an Electronic Controller for Lettuce Production in Greenhouses

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