Application and validation of an eco-friendly TLC-densitometric method for simultaneous determination of co-formulated antihypertensive agents

Lamie, Nesrine T.; Mohamed, Heba M.

doi:10.1039/c5ra08125d

Cited by 10 publications

(4 citation statements)

References 17 publications

(14 reference statements)

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“…Applying green reagents in the establishment of ecological analytical techniques has significantly increased in recent years. Green analytical chemistry (GAC) is the practice of minimizing or avoiding the use of potentially harmful chemicals in analytical processes while reducing energy use, waste creation, and other environmental impacts [ 1 , 2 ]. For quality control professionals, using environmentally friendly techniques for routine drug assessment is of the most important aims and this is what GAC aims to provide.…”

Section: Introductionmentioning

confidence: 99%

Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

et al. 2023

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Triamterene (TRI) and xipamide (XIP) mixture is used as a binary medication of antihypertension which is considered as a major cause of premature death worldwide. The purpose of this research is the quantitative and qualitative analysis of this binary mixture by green univariate and multivariate spectrophotometric methods. Univariate methods were zero order absorption spectra method (D0) and Fourier self-deconvolution (FSD), as TRI was directly determined by D0 at 367.0 nm in the range (2.00–10.00 µg/mL), where XIP show no interference. While XIP was determined by FSD at 261.0 nm in the range (2.00–8.00 µg/mL), where TRI show zero crossing. Multivariate methods were Partial Least Squares, Principal Component Regression, Artificial Neural Networks, and Multivariate Curve Resolution-Alternating Least Squares. A training set of 25 mixtures with different quantities of the tested components was used to construct and evaluate them, 3 latent variables were displayed using an experimental design. A set of 18 synthetic mixtures with concentrations ranging from (3.00–7.00 µg/mL) for TRI and (2.00–6.00 µg/mL) for XIP, were used to construct the calibration models. A collection of seven synthetic mixtures with various quantities was applied to build the validation models. All the proposed approaches quantitative analyses were evaluated using recoveries as a percentage, root mean square error of prediction, and standard error of prediction. Strong multivariate statistical tools were presented by these models, and they were used to analyze the combined dosage form available on the Egyptian market. The proposed techniques were evaluated in accordance with ICH recommendations, where they are capable of overcoming challenges including spectral overlaps and collinearity. When the suggested approaches and the published one were statistically compared, there was no discernible difference between them. The green analytical method index and eco-scale tools were applied for assessment of the established models greenness. The suggested techniques can be used in product testing laboratories for standard pharmaceutical analysis of the substances being studied.

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Section: Introductionmentioning

confidence: 99%

Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

et al. 2023

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“…In recent years, there has been a significant increase in the use of green solvents in the development of green analytical methodologies. Green analytical chemistry (GAC) is defined as the reduction or elimination of harmful chemicals used in the analytical process, as well as the reduction of energy consumption and waste production, all while maintaining system performance requirements [1][2][3]. GAC aims to provide environmentally friendly techniques for routine pharmaceutical testing, which is a major concern among quality control analysts.…”

Section: Introductionmentioning

confidence: 99%

Analytical Eco-Scale for Evaluating the Uniqueness of Voltammetric Method used for Determination of Antiemetic Binary Mixture Containing Doxylamine Succinate in Presence of its Toxic Metabolite

et al. 2022

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Green analytical procedures are gaining popularity in the pharmaceutical research area as a way to reduce environmental impact and improve analyst health safety. The current work presents a green and sensitive electrochemical carbon paste electrode that has been chemically modified with zirconium dioxide and multi-walled carbon nanotubes for estimation of pyridoxine HCl (PYR) and doxylamine succinate (DOX) using the square wave voltammetric technique. Under optimum conditions, the linearity ranges were 20.00–2000.00 ng mL−1 and 2.00–20.00 µg mL−1 for both drugs in the 1st linear segment and 2nd linear segment, respectively. Stability testing assesses how the quality of a drug substance changes over time, depending on environmental and laboratory factors. DOX was found to undergo oxidative degradation when refluxed for 7 h using 30% H2O2 and the degraded product (DOX DEG) (toxic metabolite) was successfully characterized utilizing LC–MS. The developed electrode showed selectivity for the determination of binary mixture in pure form, pharmaceutical form, and in the presence of DOX DEG and common interfering molecules with good recovery. The proposed method was found to be eco-friendlier than the reported method in terms of the use of hazardous chemicals and solvents, energy consumption, and waste generation. Graphical Abstract

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“…In recent years, the use of green solvents to establish green analytical methodologies has considerably grown. In green analytical chemistry (GAC), the definition calls for a reduction or total removal of harmful chemicals used in the analytical process, a reduction in energy consumption and minimization of waste production, without compromising the requirements for optimum performance of a system [1][2][3]. Thin-layer chromatography (TLC) has emerged as a significant step towards improved separation efficiency, allowing faster analysis, shorter peaks, better resolution [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Thin-layer chromatography (TLC) has emerged as a significant step towards improved separation efficiency, allowing faster analysis, shorter peaks, better resolution [4,5]. A significant advantage of TLC over high-performance liquid chromatography (HPLC) is the ability to run multiple samples in parallel and allow 20 samples to be spotted, isolated and quantified simultaneously on a small plate (10 cm × 20 cm) [2], while the samples in HPLC should be quantitatively injected into a pre-washed and conditioned column, resulting in a delay in data acquisition [6,7].…”

Section: Introductionmentioning

confidence: 99%

Smart TLC–densitometric methods for determination of ophthalmic ternary mixture containing chloramphenicol in the presence of its synthetic precursor: Comparative eco-scaling for greenness assessment

Eissa

El-Hadi

Zaazaa

et al. 2020

JPC-J Planar Chromat

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Green analytical methods have gained a growing interest in the field of pharmaceutical research to reduce impacts on the environment and enhance analysts' health safety. Chloramphenicol (CHL), dexamethasone sodium phosphate (DSP) and tetrahydrozoline HCl (THZ) form an ophthalmic ternary mixture that is co-formulated for conjunctivitis treatment. In the present work, for time saving and higher sensitivity, two green thin-layer chromatography (TLC) methods were developed for the determination of this ophthalmic ternary mixture in the absence or presence of p-nitroacetophenone (PNA), a synthetic precursor of chloramphenicol. In both proposed methods, silica gel 60 F 254 plates were used as the stationary phase. The mobile phase used for method (A) was ethanol-water-ammonia (7.0:2.5:0.5, V/V), while, for method (B), acetonitrilewater-ammonia (10.0:3.0:0.5, V/V) was used as the mobile phase. TLC separation was followed by quantitative determination of the aforementioned drugs at wavelengths 242.0 nm and 220.0 nm. Both methods were validated in compliance with the International Conference on Harmonisation (ICH) guidelines, where both methods were found to be reliable, reproducible, and selective. Statistical comparison of the developed methods was done with a reported high-performance liquid chromatography (HPLC) method where no significant difference was found. Analytical eco-scaling depends on penalty point which was calculated to be 92, 88 and 87 for methods A, B and the reported HPLC, respectively, suggesting that the proposed methods are eco-friendlier with penalty point scoring very high on the scale than the reported one.

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Application and validation of an eco-friendly TLC-densitometric method for simultaneous determination of co-formulated antihypertensive agents

Abstract: Application and validation of eco-friendly TLC-densitometric method for simultaneous determination of co-formulated antihypertensive agents.

Cited by 10 publications

References 17 publications

Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

Analytical Eco-Scale for Evaluating the Uniqueness of Voltammetric Method used for Determination of Antiemetic Binary Mixture Containing Doxylamine Succinate in Presence of its Toxic Metabolite

Smart TLC–densitometric methods for determination of ophthalmic ternary mixture containing chloramphenicol in the presence of its synthetic precursor: Comparative eco-scaling for greenness assessment

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