Application and methodology of dissolution dynamic nuclear polarization in physical, chemical and biological contexts

Abstract

Dissolution dynamic nuclear polarization (d-DNP) is a versatile method to enhance nuclear magnetic resonance (NMR) spectroscopy. It boosts signal intensities by four to five orders of magnitude thereby providing the potential to improve and enable a plethora of applications ranging from the real-time monitoring of chemical or biological processes to metabolomics and incell investigations. This perspectives article highlights possible avenues for developments and applications of d-DNP in biochemical and physicochemical studies. It outlines how chemists, biologists and physicists with various fields of interest can transform and employ d-DNP as a powerful characterization method for their research.

Synthesis and 15N NMR Signal Amplification by Reversible Exchange of [15N]Dalfampridine at Microtesla Magnetic Fields

“…Hyperpolarization techniques can increase nuclear spin polarization by several orders of magnitude, leading to the dramatic gain in sensitivity of nuclear magnetic resonance (NMR) experiments. A number of hyperpolarization techniques have been developed including dissolution Dynamic Nuclear Polarization (d‐DNP), [1–3] Parahydrogen‐Induced Polarization (PHIP), [4–6] and Signal Amplification by Reversible Exchange (SABRE) [7,8] . d‐DNP is a versatile technique for hyperpolarization of various compounds in solution, which exploits polarization transfer from electrons of free radicals to the nuclei at low temperatures (∼1 K) and high magnetic fields (∼3 T) by microwave irradiation [1] .…”

Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian Cell Cultures

“…Much research is active in the field of mass spectrometry and concern all aspects of the analysis, from the pre-separation, to ionization, ion separation, and detection steps and too many approaches are being explored to be discussed here. As concerns NMR, dissolution dynamic nuclear polarization (dDNP) opens exciting future perspectives for high-sensitivity NMR metabolomic detection [ 158 , 159 ].…”

Reply on RC2

“…Dissolution dynamic nuclear polarization (DDNP) (Ardenkjaer-Larsen, Fridlund et al 2003, Kovtunov, Pokochueva et al 2018, Jannin, Dumez et al 2019) is a method used for hyperpolarizing nuclear spins at cryogenic temperatures (Abragam and Goldman 1978) close to 1 K -typically attained in a liquid helium-cooled cryostat at low pressures -coupled to subsequent temperature jump and detection at ambient conditions in a conventional liquid-state nuclear magnetic resonance (NMR) spectrometer. Spin hyperpolarization is here understood as the constructive alignment of large shares of nuclear spins parallel or antiparallel to a magnetic field B0.…”