Application and Equivalence Assessment for Determining Ethamsylate by Using Potassium Ferricyanide as Spectroscopic Probe Reagent

Abstract

In this paper, a novel method has been established to determine ethamsylate using potassium ferricyanide as a spectroscopic probe reagent. It has been demonstrated that Fe(III) is reduced to Fe(II) by ethamsylate, and that the formed Fe(II) reacts with potassium ferricyanide to form soluble prussian blue (KFe III [Fe II (CN)6]). Beer's law is obeyed in the range of 0.16 -24.00 µg mL -1 with the molar absorption coefficient of 2.1 × 10 4 L mol -1 cm -1 . The detection limit (3 σ/k) is 0.11 µg mL -1 . This method has been successfully applied to determine ethamsylate in pharmaceutical and serum samples with satisfactory results, and presented quite satisfactory credibility during method equivalence assessment.

Highly sensitive electrochemiluminescence determination of etamsylate using a low‐cost electrochemical flow‐through cell based on a tris(2, 2'‐bipyridyl)ruthenium(II)–Nafion‐modified carbon paste electrode

“…To control haemorrhaging, ETM may be given to adults orally or parentrally, either intramuscularly or intravenously at a dose of 250–500 mg after surgery . Because of its great therapeutical importance and widespread use, several procedures have been reported for the determination of ETM in biological fluids and pharmaceutical formulations, including spectrophotometry , high‐performance TLC , high‐peformanceliquid chromatography , electrochemical methods , chemiluminescence , derivative synchronous fluorometry and capillary electrophoresis . However, some spectroscopic techniques are time‐consuming and laborious, and chromatographic techniques are slow and expensive and complicated instruments are also required.…”