Application and Analysis in the Biomedicine Field Using Digital Holographic Technology

Yunxin, 王云新 Wang; Dayong, 王大勇 Wang; Yishu, 杨怡姝 Yang; Jie, 赵洁 Zhao; Liting, 欧阳丽婷 Ouyang; Xiangqian, 肖向茜 Xiao; Lu, 戎路 Rong

doi:10.3788/cjl201441.0209002

Cited by 2 publications

(1 citation statement)

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“…It proves that the dual-wavelength DHM allows a faster imaging, which does not rely on the surrounding pixels to correct the phase discontinuities, but simply compares two singlewavelength phases. Dual-wavelength DHM has been widely applied in the fields of surface topography measurement [20][21][22], cell imaging [23,24], 3D particle imaging [25,26], transparent medium physical quantity measurement [27][28][29], and so on. Due to the limitation of the dual-wavelength DHM technique, more noise will be introduced with the increase in synthesis wavelength and the expanding of the sample range.…”

Section: Introductionmentioning

confidence: 99%

Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy

Lin

Dai

et al. 2018

International Journal of Optics

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A dual-wavelength digital holographic microscopy with premagnification is proposed to obtain the object surface measurements over the large gradient. The quantitative phase images of specimens are captured in high precision by the processing of filtering and phase compensation. The phase images are acquired without phase unwrapping, which is necessary in traditional digital holographic microscopy; thereby the proposed system can greatly increase the speed of reconstruction. The results of numerical simulation and optical experiments demonstrated that the reconstructed speed increased by 37.9 times, and the relative error of measurement is 4% compared with the traditional holographic microscopy system. It means that the proposed system can directly acquire the higher quality quantitative phase distribution for specimens.

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