Application advances and prospects of CRISPR/Cas9 genome editing in nematodes

Cao, Xuwen; Zhang, LiuSuo

doi:10.1360/n052017-00284

Cited by 2 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nematodes represent about 80% of the number of multicellular animals in numbers on the earth, and are distributed in almost all ecological niches, from terrestrial soil to the ocean (Cao and Zhang, 2018;Eisenhauer and Guerra, 2019;van den Hoogen et al, 2019;Xie et al, 2020). The phylum Nematoda comprises more than 25,000 described species, and as many as one million species still need to be described (Hugot et al, 2001;Abad et al, 2008).…”

Section: Evo-devo and Adaptation To The Global Climate Change In Marine Model Nematode (Figure 12)mentioning

confidence: 99%

“…More than 4,100 and 5,000 species of plant and animal-parasitic nematodes, respectively, have been documented to date (Kikuchi et al, 2017). Over two billion humans are infected with parasitic nematodes (International Helminth Genomes Consortium, 2019;Else et al, 2020), and parasitic nematodes also cause diseases in domesticated animals, marine fishes, and crop plants, resulting in hundreds of billions of dollars economic loss every year (Abad et al, 2008;Cao and Zhang, 2018). Collectively, nematodes not only play major roles in terrestrial and ocean ecosystems, but also cause significant parasitic infections in humans, animals, and plants.…”

Section: Evo-devo and Adaptation To The Global Climate Change In Marine Model Nematode (Figure 12)mentioning

confidence: 99%

See 1 more Smart Citation

Biodiversity-based development and evolution: the emerging research systems in model and non-model organisms

Zhao

Gao

et al. 2021

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

Evolutionary developmental biology, or Evo-Devo for short, has become an established field that, broadly speaking, seeks to understand how changes in development drive major transitions and innovation in organismal evolution. It does so via integrating the principles and methods of many subdisciplines of biology. Although we have gained unprecedented knowledge from the studies on model organisms in the past decades, many fundamental and crucially essential processes remain a mystery. Considering the tremendous biodiversity of our planet, the current model organisms seem insufficient for us to understand the evolutionary and physiological processes of life and its adaptation to exterior environments. The currently increasing genomic data and the recently available gene-editing tools make it possible to extend our studies to non-model organisms. In this review, we review the recent work on the regulatory signaling of developmental and regeneration processes, environmental adaptation, and evolutionary mechanisms using both the existing model animals such as zebrafish and Drosophila, and the emerging nonstandard model organisms including amphioxus, ascidian, ciliates, single-celled phytoplankton, and marine nematode. In addition, the challenging questions and new directions in these systems are outlined as well.

show abstract

Section: Evo-devo and Adaptation To The Global Climate Change In Marine Model Nematode (Figure 12)mentioning

confidence: 99%

Section: Evo-devo and Adaptation To The Global Climate Change In Marine Model Nematode (Figure 12)mentioning

confidence: 99%

Biodiversity-based development and evolution: the emerging research systems in model and non-model organisms

Zhao

Gao

et al. 2021

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…As part of their symbiotic and entomopathogenic lifecycle, all Xenorhabdus must colonize and be transported by a nematode host, suppress insect immunity, establish a community within and consume the cadaver, and support reproduction of the nematode to ensure future transport [10]. Many will also compete or cooperate with other resident or transient hermaphroditum, currently the most genetically tractable steinernematid [24,120,25].…”

Section: Discussionmentioning

confidence: 99%

“…Our analysis centred around seven related Xenorhabdus strains, chosen based on their close phylogenetic proximity to X. griffiniae . Strains of this Xenorhabdus species are particularly relevant to the development of laboratory model systems used to study nematode-bacteria symbiosis because they are symbionts of S. hermaphroditum, currently the most genetically tractable steinernematid [24, 120, 25].…”

Section: Discussionmentioning

confidence: 99%

Analyses ofXenorhabdus griffiniaegenomes reveal two distinct sub-species that display intra-species variation due to prophages

Heppert,

Awori,

Cao

et al. 2024

Preprint

View full text Add to dashboard Cite

Nematodes of the genus Steinernema and their Xenorhabdus bacterial symbionts are lethal entomopathogens that are useful in the biocontrol of insect pests, as sources of diverse natural products, and as research models for mutualism and parasitism. Xenorhabdus play a central role in all aspects of the Steinernema lifecycle, and a deeper understanding of their genomes therefore has the potential to spur advances in each of these applications. Results: Here, we report a comparative genomics analysis of Xenorhabdus griffiniae, including the symbiont of Steinernema hermaphroditum nematodes, for which genetic and genomic tools are being developed. We sequenced and assembled circularized genomes for three Xenorhabdus strains: HGB2511, ID10 and TH1. We then determined their relationships to other Xenorhabdus and delineated their species via phylogenomic analyses, concluding that HGB2511 and ID10 are Xenorhabdus griffiniae while TH1 is a novel species. These additions to the existing X. griffiniae landscape further allowed for the identification of two subspecies within the clade. Consistent with other Xenorhabdus, the analysed X. griffiniae genomes each encode a wide array of antimicrobials and virulence-related proteins. Comparative genomic analyses, including the creation of a pangenome, revealed that a large amount of the intraspecies variation in X. griffiniae is contained within the mobilome and attributable to prophage loci. In addition, CRISPR arrays, secondary metabolite potential and toxin genes all varied among strains within the X. griffiniae species. Conclusions: Our findings suggest that phage-related genes drive the genomic diversity in closely related Xenorhabdus symbionts, and that these may underlie some of the traits most associated with the lifestyle and survival of entomopathogenic nematodes and their bacteria: virulence and competition. This study establishes a broad knowledge base for further exploration of not only the relationships between X. griffiniae species and their nematode hosts but also the molecular mechanisms that underlie their entomopathogenic lifestyle.

show abstract

Application advances and prospects of CRISPR/Cas9 genome editing in nematodes

Cited by 2 publications

References 22 publications

Biodiversity-based development and evolution: the emerging research systems in model and non-model organisms

Biodiversity-based development and evolution: the emerging research systems in model and non-model organisms

Analyses ofXenorhabdus griffiniaegenomes reveal two distinct sub-species that display intra-species variation due to prophages

Contact Info

Product

Resources

About