Applicability of the Charm II system for monitoring antibiotic residues in manure-based composts

Kwon, Soon Wook; Owens, Gary; Ok, Yong Sik; Lee, D.B.; Jeon, Weon Tai; Kim, Jae Gyoon; Kim, K.-R.

doi:10.1016/j.wasman.2010.08.018

Cited by 45 publications

(18 citation statements)

References 30 publications

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“…At present, there is no regulated guideline to limit the concentrations of TCs in composts in China. Kwon et al (2011) used the guideline concentration that appeared in the 'Official Standard of Feeds' under the 'Control of Livestock and Fish Feed Act' in Korea (800 μg/kg for TCs) as a control point to monitor the residues of the target veterinary antibiotics in manure-based compost. According to this guideline concentration, the compost sample from Putian exceeded the value.…”

Section: Occurrence Of Tetracyclines In Manure-based Compostmentioning

confidence: 99%

Fate of tetracyclines in swine manure of three selected swine farms in China

Qiao

Chen²,

et al. 2012

Journal of Environmental Sciences

112

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Veterinary antibiotics can enter the environment due to the common practice of land application of manure from treated animals. The environmental fate of tetracyclines in swine manure after composting and field application remains largely unknown. This study analyzed the concentrations of tetracyclines in manure, manure-based compost and compost amended soil in selected swine farms from Beijing, Jiaxing and Putian, China to determine the dilution effects of antibiotics when released into the soil environment. The results demonstrate that residues of antibiotics were detected in all samples and chlortetracycline as well as its degradation products should be regarded critically concerning their potential ecotoxicity. Application of manure-based compost to soil could reduce the possible risk posed by antibiotic contamination, but the trigger value of 100 μg/kg was still exceeded in soil samples (776.1 μg/kg dw) from Putian City after application of compost. Field studies such as the present one can help to improve the routine administration of antibiotic-containing composted manure.

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Section: Occurrence Of Tetracyclines In Manure-based Compostmentioning

confidence: 99%

Fate of tetracyclines in swine manure of three selected swine farms in China

Qiao

Chen²,

et al. 2012

Journal of Environmental Sciences

112

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“…Therefore, reliable quantification of antibiotics excreted in feces and urine is needed assess the environmental impact of the livestock industry. The microbial- or immuno-assays used to test milk and meat may yield false positive identification in manures due to matrix interferences [12], often fail to differentiate a parent compound from its metabolite(s) [13], suffer low detection sensitivity [14]–[16], and provide only semi-quantitative results [17]. Hence, there is a need for both qualitative and quantitative analytical methods to better assess the fate and impact of antibiotics in animal products, manure, and manure-impacted environments.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a UPLC-MS/MS Method to Monitor Cephapirin Excretion in Dairy Cows following Intramammary Infusion

et al. 2014

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Cephapirin, a cephalosporin antibiotic, is used by the majority of dairy farms in the US. Fecal and urinary excretion of cephapirin could introduce this compound into the environment when manure is land applied as fertilizer, and may cause development of bacterial resistance to antibiotics critical for human health. The environmental loading of cephapirin by the livestock industry remains un-assessed, largely due to a lack of appropriate analytical methods. Therefore, this study aimed to develop and validate a cephapirin quantification method to capture the temporal pattern of cephapirin excretion in dairy cows following intramammary infusion. The method includes an extraction with phosphate buffer and methanol, solid-phase extraction (SPE) clean-up, and quantification using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The LOQ values of the developed method were 4.02 µg kg−1 and 0.96 µg L−1 for feces and urine, respectively. This robust method recovered >60% and >80% cephapirin from spiked blank fecal and urine samples, respectively, with acceptable intra- and inter-day variation (<10%). Using this method, we detected trace amounts (µg kg−1) of cephapirin in dairy cow feces, and cephapirin in urine was detected at very high concentrations (133 to 480 µg L−1). Cephapirin was primarily excreted via urine and its urinary excretion was influenced by day (P = 0.03). Peak excretion (2.69 mg) was on day 1 following intramammary infusion and decreased sharply thereafter (0.19, 0.19, 0.08, and 0.17 mg on day 2, 3, 4, and 5, respectively) reflecting a quadratic pattern of excretion (Quadratic: P = 0.03). The described method for quantification of cephapirin in bovine feces and urine is sensitive, accurate, and robust and allowed to monitor the pattern of cephapirin excretion in dairy cows. This data will help develop manure segregation and treatment methods to minimize the risk of antibiotic loading to the environment from dairy farms.

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“…Michael (2003) Kwon (2011) Charm Ⅱ HPLC-ACPI-MS/MS erythromycin, oleandomycin, roxithromycin, salinomycin, tiamulin, tetracyclines , sulfonamides macrolides (Michael et al, 2003;Kwon et al, 2011).…”

Section: -90%mentioning

confidence: 99%

Development of Simultaneous Analytical Method of Veterinary Antibiotics in Manure using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Chung¹,

Lee²,

Rahman³

et al. 2017

Korean Journal of Environmental Agriculture

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BACKGROUND:The current study was to monitor of 9 veterinary antibiotics (ceftiofur, clopidol, florfenicol, sulfamethazine, sulfamethoxazole, sulfathiazole, tetracycline, tiamulin, and tylosin) in manure using liquid chromatographytandem mass spectrometry (LC-MS/MS) in positive and negative electrospray ionization mode. METHODS AND RESULTS: Sample preparation was carried out using Mcllvaine buffer and citrate salts to adjust the pH of the sample followed by purification with dispersive solid phase extraction (d-SPE). Separation of analytes during LC-MS/MS analysis was conducted using an Eclipse Plus C18 column and the mobile phase was in gradient mode with, 0.1% formic acid and 5 mM ammonium formate in methanol (A) and 0.1% formic acid and 5 mM ammonium formate in distilled water (B). The linearity of the matrix-matched calibrations of all tested antibiotics was good, with R 2 determination coefficients ≥ 0.9920. The limit of detection (LOD) and quantifications (LOQ) were 0.1-67.0 g/kg and 0.4-200.0 g/kg, μ μ respectively. Analysis of 13 solid and liquid manure samples taken from the Republic of Korea revealed concentrations less than 0.7 g/kg for tiamulin, 1497.6 μ μ g/kg for sulfamethazine. CONCLUSION: To monitor 9 veterinary antibiotics from manure samples in 13 provincial areas throughout the Republic of Korea, an analytical method was developed. The developed method was fully validated and successfully applied for monitoring various veterinary antibiotics in manure samples.

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Applicability of the Charm II system for monitoring antibiotic residues in manure-based composts

Cited by 45 publications

References 30 publications

Fate of tetracyclines in swine manure of three selected swine farms in China

Fate of tetracyclines in swine manure of three selected swine farms in China

Development and Validation of a UPLC-MS/MS Method to Monitor Cephapirin Excretion in Dairy Cows following Intramammary Infusion

Development of Simultaneous Analytical Method of Veterinary Antibiotics in Manure using Liquid Chromatography Coupled with Tandem Mass Spectrometry

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