Applicability of Homologous Fibrin Sealant in Bone Repair: An integrative Review

Pomini, Karina Torres; Buchaim, Daniela Vieira; Shindo, João Vitor Tadashi Cosin; Flato, Uri Adrian Prync; Rosso, Marcelie Priscila de Oliveira; Andreo, Jesús Carlos; Coletta, Bruna Botteon Della; Duarte, Janaina Costa Marangon; Buchaim, Rogério Leone

doi:10.22161/ijaers.673

Cited by 4 publications

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“…The increase in bone disease rates triggered by fractures, pseudoarthrosis, osteoporosis and or tumor resection has aroused interest in the development of new biomaterials in order to restore the morphological and functional integrity of the lost tissue [18,19].…”

Section: Discussionmentioning

confidence: 99%

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

et al. 2020

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Tissue engineering represents a promising alternative for reconstructive surgical procedures especially for the repair of bone defects that do not regenerate spontaneously. The present study aimed to evaluate the effects of the elastin matrix (E24/50 and E96/37) incorporated with hydroxyapatite (HA) or morphogenetic protein (BMP) on the bone repair process in the distal metaphysis of rat femur. The groups were: control group (CG), hydrolyzed elastin matrix at 50˚C/24h (E24/50), E24/50 + HA (E24/50/HA), E24/50 + BMP (E24/50/ BMP), hydrolyzed elastin matrix at 37˚C/96h (E96/37), E96/37 + HA (E96/37/HA), E96/37 + BMP (E96/37/BMP). Macroscopic and radiographic analyses showed longitudinal integrity of the femur in all groups without fractures or bone deformities. Microtomographically, all groups demonstrated partial closure by mineralized tissue except for the E96/37/HA group with hyperdense thin bridge formation interconnecting the edges of the ruptured cortical. Histologically, there was no complete cortical recovery in any group, but partial closure with trabecular bone. In defects filled with biomaterials, no chronic inflammatory response or foreign body type was observed. The mean volume of new bone formed was statistically significant higher in the E96/37/HA and E24/50 groups (71.28 ± 4.26 and 66.40 ± 3.69, respectively) than all the others. In the confocal analysis, it was observed that all groups

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Section: Discussionmentioning

confidence: 99%

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

et al. 2020

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“…It is also worth mentioning that the present study proposed a new proportionality of the fibrinogen component of the heterologous fibrin biopolymer, in order to reduce the stiffness and high density of the fibrin mesh, as seen in our previous studies [ 25 , 30 ]. Thus, it is believed that it enables a tissue construction with pore morphology compatible with the native bone matrix, enabling the incorporation and adsorption of bioactive molecules and cells.…”

Section: Discussionmentioning

confidence: 89%

“…However, a new approach to treatment frequency has been required for future research due to greater convenience, and to the need to be financially viable, allowing for the use of this technique in clinical practice. Allied to this, the scientific literature together with our previous results have pointed out the need to change the proportionality of the fibrinogen component of the heterologous fibrin biopolymer, in order to achieve a less dense three-dimensional mesh, as it is believed that this way provides a microenvironment more conducive to cell migration [ 21 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Tissue Bioengineering with Fibrin Scaffolds and Deproteinized Bone Matrix Associated or Not with the Transoperative Laser Photobiomodulation Protocol

et al. 2023

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Extending the range of use of the heterologous fibrin biopolymer, this pre-clinical study showed a new proportionality of its components directed to the formation of scaffold with a lower density of the resulting mesh to facilitate the infiltration of bone cells, and combined with therapy by laser photobiomodulation, in order to accelerate the repair process and decrease the morphofunctional recovery time. Thus, a transoperative protocol of laser photobiomodulation (L) was evaluated in critical bone defects filled with deproteinized bovine bone particles (P) associated with heterologous fibrin biopolymer (HF). The groups were: BCL (blood clot + laser); HF; HFL; PHF (P+HF); PHFL (P+HF+L). Microtomographically, bone volume (BV) at 14 days, was higher in the PHF and PHFL groups (10.45 ± 3.31 mm3 and 9.94 ± 1.51 mm3), significantly increasing in the BCL, HFL and PHFL groups. Histologically, in all experimental groups, the defects were not reestablished either in the external cortical bone or in the epidural, occurring only in partial bone repair. At 42 days, the bone area (BA) increased in all groups, being significantly higher in the laser-treated groups. The quantification of bone collagen fibers showed that the percentage of collagen fibers in the bone tissue was similar between the groups for each experimental period, but significantly higher at 42 days (35.71 ± 6.89%) compared to 14 days (18.94 ± 6.86%). It can be concluded that the results of the present study denote potential effects of laser radiation capable of inducing functional bone regeneration, through the synergistic combination of biomaterials and the new ratio of heterologous fibrin biopolymer components (1:1:1) was able to make the resulting fibrin mesh less dense and susceptible to cellular permeability. Thus, the best fibrinogen concentration should be evaluated to find the ideal heterologous fibrin scaffold.

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“…A new fi brin sealant, currently called fi brin biopolymer due to its diversity of applications and properties, was developed in Brazil by a group of researchers from CEVAP (Center of the Studies of Venoms and Venomous Animals -UNESP Botucatu, SP) without the presence of human blood. This bioproduct has been shown to be effective in a variety of preclinical and clinical situations with therapeutic potential such as repair of nerve damage [13][14][15][16][17][18], correction of bone defects [8,[19][20][21], treatment of venous ulcers [22] and stem cell scaffolds [23].…”

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confidence: 99%

Clinical potential in modern medicine of fibrin glues as drug delivery system

Pilon¹,

Buchaim²,

Hamzé³

et al. 2020

Open J Biol Sci

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In an attempt to achieve greater therapeutic effi ciency of products, especially in the pharmacological area such as drugs in general, the drug delivery system that controls the distribution of substances through macromolecular carriers has been developed with the primary objective of optimizing their delivery to target locations [1]. Records in the scientifi c literature of pioneering controlled drug delivery studies date back to the 1960s at Harvard with the in vivo implantation of a silicone tube that became a drug delivery device, in this case anesthetic gases, at a constant rate of release. [2]. Clinical use was approved in the 1980s and 1990s with the use of microscopic degradable polymer depot Drug Delivery Systems (DDS) but it can be cited that clinical success occurred in the 2000s with nanoscopic products [3]. From this period a rapid evolution occurred and the range of use at both molecular and supramolecular levels was expanded [4]. The clinical use of nanotechnology in health has been a major revolution, with controlled drug release being one of its examples in treating various types of cancer, manufacturing vaccines and treating fungal infections using liposomes as a component [5].

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Applicability of Homologous Fibrin Sealant in Bone Repair: An integrative Review

Cited by 4 publications

References 0 publications

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

Tissue Bioengineering with Fibrin Scaffolds and Deproteinized Bone Matrix Associated or Not with the Transoperative Laser Photobiomodulation Protocol

Clinical potential in modern medicine of fibrin glues as drug delivery system

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