Applicability of emanating volatile organic compounds from various forensic specimens for individual differentiation

Brown, Jessica S.; Prada, Paola A.; Curran, Allison M.; Furton, Kenneth G.

doi:10.1016/j.forsciint.2013.01.008

Cited by 41 publications

(44 citation statements)

References 32 publications

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“…Solid phase microextraction (SPME) coupled to gas chromatography/mass spectrometry (GC/MS) is one of the most widely used hyphenated techniques employed for sampling, extraction and enrichment of volatiles from salivary headspace (HS). Volatile organic compounds (VOCs) in saliva are mainly blood‐derived products or byproducts of bacterial metabolism . In only several studies focused on obtaining volatile profiles using HS‐SPME‐GC/MS, saliva samples were incubated at room temperature for 24 h, prior to extraction of volatiles which lasted for 21 h .…”

Section: Introductionmentioning

confidence: 99%

Investigation of bacterial viability from incubated saliva by application of flow cytometry and hyphenated separation techniques

et al. 2017

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The aim of the study was determination of bacterial viability in saliva samples and finding a correlation between microbiological and volatile profiles of saliva depending on incubation time. Bacteria colonizing healthy oral cavities were also identified. Twelve healthy adults donated unstimulated saliva samples. Flow cytometry, optical density measurements and colony-forming unit (CFU) counting method were employed for analyses of native and inoculated saliva after 0, 1, 2, 24, and 48 h of incubation. Volatile profiles were acquired using headspace-solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS). Oral bacteria were the most viable within 2 h after collection of saliva. Extension of incubation time to 48 h caused considerable decrease in live bacteria counts and sharp increase in dead bacteria counts. The most prevalent strain was Sphingomonas paucimobilis (26.67%). The number of volatiles raised from 5 to 27 with incubation time and most of them were putrefaction products, such as methanethiol, indole and pyrrole. HS-SPME-GC/MS method is insufficient for volatile profiling of "fresh" saliva and should be directed rather to investigation of bacterial metabolites.

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Section: Introductionmentioning

confidence: 99%

Investigation of bacterial viability from incubated saliva by application of flow cytometry and hyphenated separation techniques

et al. 2017

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“…Previous research using the direct collection method discovered that human scent could be discriminated between individuals by isolating those VOCs that were found to be common among the samples collected [3,14,15,17,18]. For this study, the authors' classified a compound as being common if it was present in at least two out of the three samples that were directly collected (D-H) from each subject.…”

Section: Human Scent Results From Population Studymentioning

confidence: 99%

“…Helium was used as the carrier gas at 1 mL/min. Using the Varian MS Workstation Software, Version 6.6 (Service Pack 1), all compounds detected were verified and quantitated using an external calibration curve with standards that were purchased from Sigma Aldrich (St. Louis, MO) and recognized as human scent compounds [3,[13][14][15][16][17][18][19]37,40,43,44]. They included: octane; furfural; 2-furanmethanol; nonane; heptanal; 6-methyl-5-hepten-2-one; octanal; benzyl alcohol; (E)-2-octenal; 1-octanol; undecane; octanoic acid methyl ester; 1-nonanol; 2-decanone; dodecane; hexanedioic acid dimethyl ester; tridecane; undecanal; tetradecane; (E)-6,10-dimethyl-5,9-undecadien-2-one (geranyl acetone); pentadecane; and heptadecane.…”

Section: Human Scent Analysis Using Hs-spme/gc-msmentioning

confidence: 99%

“…These skin cells contain volatile organic compounds (VOCs) associated with human scent that consists of various chemical functional groups, such as carboxylic acids, alcohols, aldehydes, aliphatics/aromatics, esters, halides, heterocyclics, ketones, and nitrogen-and sulfur-containing compounds [2,3,[13][14][15][16][17][18][19]. Epithelial skin cells also contain nuclear and mitochondrial deoxyribonucleic acid (nDNA; mtDNA) that can be used to obtain an individual's DNA profile or provide maternal relatedness information.…”

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confidence: 99%

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An investigation into the concurrent collection of human scent and epithelial skin cells using a non-contact sampling device

Caraballo

Mendel

Holness

et al. 2016

Forensic Science International

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“…Additionally, research was also performed on the scent collected from other external body locations, as well as from various biological specimens, such as fingernails, hair, saliva, breath, blood and urine, to determine if the VOCs liberated from these locations/materials could also be used to differentiate individuals. The results showed discriminating powers, within a single-specimen type, of more than 98.9% of the individuals being distinguishable [49]. The ability for canines to differentiate between identical twins was explored by Hepper & Wells [50], as well as Harvey et al [51].…”

Section: (C) Human Scentmentioning

confidence: 99%

Advances in the use of odour as forensic evidence through optimizing and standardizing instruments and canines

Furton

Caraballo

Cerreta

et al. 2015

Phil. Trans. R. Soc. B

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This paper explores the advances made in identifying trace amounts of volatile organic compounds (VOCs) that originate from forensic specimens, such as drugs, explosives, live human scent and the scent of death, as well as the probative value for detecting such odours. The ability to locate and identify the VOCs liberated from or left by forensic substances is of increasing importance to criminal investigations as it can indicate the presence of contraband and/or associate an individual to a particular location or object. Although instruments have improved significantly in recent decades—with sensitivities now rivalling that of biological detectors—it is widely recognized that canines are generally still more superior for the detection of odourants due to their speed, versatility, ruggedness and discriminating power. Through advancements in the detection of VOCs, as well as increased standardization efforts for instruments and canines, the reliability of odour as evidence has continuously improved and is likely to continue to do so. Moreover, several legal cases in which this novel form of evidence has been accepted into US courts of law are discussed. As the development and implementation of best practice guidelines for canines and instruments increase, their reliability in detecting VOCs of interest should continue to improve, expanding the use of odour as an acceptable form of forensic evidence.

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Applicability of emanating volatile organic compounds from various forensic specimens for individual differentiation

Cited by 41 publications

References 32 publications

Investigation of bacterial viability from incubated saliva by application of flow cytometry and hyphenated separation techniques

Investigation of bacterial viability from incubated saliva by application of flow cytometry and hyphenated separation techniques

An investigation into the concurrent collection of human scent and epithelial skin cells using a non-contact sampling device

Advances in the use of odour as forensic evidence through optimizing and standardizing instruments and canines

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