Applicability of anatid and galliform microsatellite markers to the genetic diversity studies of domestic geese (Anser anser domesticus) through the genotyping of the endangered zatorska breed

Abstract

BackgroundThe lack of a sufficient number of molecular markers seriously limits the cognition of genetic relationships within and between populations of many species. Likewise, the genetic diversity of domestic goose (Anser anser domesticus), with a great number of breeds throughout the world, remains poorly understood at the molecular level.FindingsThirty-five goose, seventeen duck and eight chicken microsatellite primer pairs were screened for their utility in the cross-species amplification on DNA from 96 individuals of Zatorska breed of domestic geese. Twenty-seven of 42 amplifying primer pairs revealed length-polymorphic products, but three of them were difficult to score. Fifteen primer pairs amplifying the same length product across all individuals. One polymorphic microsatellite locus was assigned by genotyping of known sex individuals to the Z-chromosome.ConclusionsWe present a set of 24 polymorphic microsatellite markers useful for population genetic studies of the domestic goose. Another 15 markers were classified as monomorphic, but they might also be suitable for the assessment of genetic diversity in geese.

Genetic diversity of Tesedik Goose

“…The observed heterozygosity over all loci was similar or lower than mean expected. The mean observed heterozygosity in Tesedik Goose was similar as in other European geese (Aliczki et al, 2007;Andres and Kapkowska, 2011;Noreikiene et al, 2012), but higher than in Suchovska and Slovak Goose breeds (Mindek et al, 2014).…”

“…All estimated microsatellite loci were polymorphic as was published in previously studies (Aliczki 2007;Weiß et al 2008;Andres and Kapkowska, 2011), with a varying number of alleles and expect for Aalμ1 used in diversity study were suitable according to number of detected loci over the population. The number of alleles detected in loci TTUCG5, CKW21, Bcaμ1 was similar as in Zatorska breed (Andres and Kapkowska, 2011). Considerable lower number of detected alleles were in CKW21, Aalμ1, Ans2 and Ans25 loci, compare to other published papers (Liu et al, 2006;Aliczki 2007;Weiß et al 2008).…”

“…Currently there are known same microsatellite markers isolated and evaluated in the wild form of geese as Greylag Goose Anser anser (Weiß et al, 2008), Canada Goose Branta canadensis L. (Cathey et al, 1998), Swan Goose Anser cygnoides L. (Tu et al, 2006, Li et al, 2007, White-fronted Goose Anser albifrons (Fields et al, 1997), Pink-footed Goose Anser brachyrhynchus (Noreikiene, 2012). Anatidae specific microsatellite markers for study of genetic diversity were used in Chinese (Liu et al, 2006, Tu et al, 2006, Li et al, 2007, Hungarian, Embden (Aliczki, 2007) and Zatorska (Andres and Kapkowska, 2011) geese breeds.…”

Genetic Diversity of Slovak Origin Geese Populations

“…Significant deficiency of heterozygotes occurred in locus TTUGC5 (Slovak), Ans2 (Slovak) and Bcaμ1 (Suchovska, Tesedik), (Table 3). Liu et al, 2006;Aliczki 2007;Wieß et al 2008;Andres and Kapkowska, 2011). Barker (1994) suggested that microsatellite loci used in genetic distance studies should have more than four alleles in order to reduce the distance estimate standard error.…”

Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)

“…It is commonly accepted that PIC values > 0.50 are characteristic of the most useful markers, 0.25 < PIC > 0.50 of those moderately informative, and PIC < 0.25 of the less informative (Andres and Kapkowska, 2011). Thus, the loci optimized in this study can thus be considered moderate to highly informative.…”