Applicability of an optimized non-conventional flow cytometry method to detect anti-Trypanosoma cruzi immunoglobulin G for the serological diagnosis and cure assessment following chemotherapeutic treatment of Chagas disease

Target Product Profile (TPP) for Chagas Disease Point-of-Care Diagnosis and Assessment of Response to Treatment

“…Novel nucleic acid amplification approaches such as loop-mediated isothermal amplification, which are currently undergoing standardization and validation for other trypanosomatide infections, should be explored in Chagas disease diagnosis [ 32 , 33 ]. While flow cytometry has been shown to be a good platform, with high sensitivity and specificity for Chagas disease diagnosis and post-therapeutic cure monitoring [ 34 , 35 ], the use of flow cytometry-based devices to develop "point-of-care" TTPs does not seem to be a feasible approach, mainly considering the complexity and the nonportable nature of these devices.…”

FC-TRIPLEX Chagas/Leish IgG1: A Multiplexed Flow Cytometry Method for Differential Serological Diagnosis of Chagas Disease and Leishmaniasis

“…Previous studies conducted by our group proved the applicability and utility of FC for diagnosis and clinical monitoring of CH and leishmaniasis [ 12 – 15 , 17 , 29 , 30 ]. First, we demonstrated the applicability of FC for serological testing for CH, using live trypomastigote forms of T .…”

“…Similarly, the thresholds for CH and LCL were set to 50% and 60% at dilutions of 1:2,000 and 1:1,000, respectively. Such thresholds have also been determined in previously published experiments and for each of the single clinical conditions separately [ 13 , 15 ]. ROC analyses and evaluation of the performance of each of the parasite preparations and IgG1 reactivity in the different patient groups, as described in Population, Materials, and Methods, demonstrated that PPFP values >60% for L .…”

“…cruzi at 1:2,000; LCL and NI, anti- L . braziliensis at 1:1,000) using the cut-off edges (dotted lines), previously described by Garcia et al [ 13 ], Matos et al [ 15 ], and Pereira et al [ 12 ]. PPFP = 60% for anti- L .…”

“…In subsequent studies, we verified the applicability and performance of a FC-based method for detecting anti-fixed epimastigote IgG antibodies in sera from individuals infected with T . cruzi [ 14 , 15 ]. Additionally, we reported an upgraded FC technology using fixed Leishmania (Leishmania) amazonensis promastigotes as antigens for serological diagnosis of cutaneous leishmaniasis [ 12 ].…”