Apple WRKY transcription factor MdWRKY56 positively modulates drought stress tolerance

Duan, Dingyue; Yi, Ran; Ma, Yi; Dong, Qinglong; Mao, Ke; Ma, Fengwang

doi:10.1016/j.envexpbot.2023.105400

Cited by 15 publications

(7 citation statements)

References 58 publications

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“…Therefore, it can be observed from the phenotype that OE-ChaWRKY40 improved the drought tolerance of hybrid hazelnut. Simultaneously, it was found that the proline content and transcriptional levels of ChaP5CS in OE-ChaWRKY40 plants were significantly higher than those in the control under drought stress, which was consistent with the results in apple [54]. Research has suggested that the concentrations of MDA and EL in transgenic OE-FcWRKY40 tobacco were remarkably lower than those in WT plants under salt stress [25].…”

Section: Discussionsupporting

confidence: 85%

ChaWRKY40 Enhances Drought Tolerance of ‘Dawei’ Hazelnuts by Positively Regulating Proline Synthesis

Zhang,

Chao,

Qiu

et al. 2024

Forests

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Hazelnuts are among the most important nuts worldwide. Drought has severely restricted the development of the hazelnut industry in the wake of global warming and lack of water resources. Δ-1-pyrroline-5-carboxylic acid synthase (P5CS) is closely related to drought stress as the rate-limiting enzyme of proline synthesis. WRKY40 had been proven to be an important transcription factor regulating drought tolerance in several plants. In this study, the hybrid hazelnut ‘Dawei’ exhibiting drought tolerance was used as the test material. Tests for simulated drought stress and ChaWRKY40 overexpression, and the yeast one-hybrid assay were performed. The results showed that the relative water content of leaves gradually decreased, but the proline content, electrolyte leakage, and expression of ChaWRKY40 and ChaP5CS increased with increasing PEG-6000 concentration in the leaves. A transient ChaWRKY40 overexpression trial indicated that overexpression of ChaWRKY40 improved the proline content and the transcription level of ChaP5CS. The Y1H experiment suggested that ChaWRKY40 directly binds to the W-box-acting element (W-box) on the promoter of ChaP5CS. In conclusion, ChaWRKY40 may increase the proline content by positively regulating the expression of the ChaP5CS gene, thereby improving the drought resistance of hazelnuts.

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Section: Discussionsupporting

confidence: 85%

ChaWRKY40 Enhances Drought Tolerance of ‘Dawei’ Hazelnuts by Positively Regulating Proline Synthesis

Zhang,

Chao,

Qiu

et al. 2024

Forests

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“…MbWRKY5 and MdWRKY30 enhance tolerance to drought and salt in tobacco and Arabidopsis, respectively, by regulating the expression levels of genes associated with stress responses; these genes are involved in alleviating oxidative stress and maintaining the integrity of the cell membrane (Han et al, 2019;Dong et al, 2020). MdWRKY56 has previously been identi ed as a positive regulator of drought tolerance, and transgenic apple plants overexpressing the gene encoding this TF have a higher PRO content and antioxidant enzyme activities (Duan et al, 2023). However, no key TFs responsive to salt-alkali stress have been reported.…”

Section: Discussionmentioning

confidence: 99%

Physiological studies and transcriptomic analysis reveal the mechanism of saline–alkali stress resistance of Malus sieversii f. niedzwetzkyan

Jiang,

Yang,

et al. 2024

Preprint

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Apple (Malus) is one of the world’s most economically significant fruit varieties. The inability of apple trees to tolerate saline–alkali soil has long limited their growth and yield. Malus sieversii f. niedzwetzkyan is a wild species capable of growing on saline–alkali soil in Xinjiang.Here, we conducted a hydroponic experiment in which M. niedzwetzkyana and M. domestica “Royal Gala” seedlings were subjected to 150 mM saline–alkali stress. Physiological data indicated that the saline–alkali resistance of M. niedzwetzkyana was higher than that of M. “Royal Gala,” as the concentrations of ROS were lower and Na+/K+ ratios were higher in M. niedzwetzkyana than in M. “Royal Gala” under saline–alkali stress. Transcriptome analysis was conducted on the leaves and roots of M. niedzwetzkyana at different time points under saline–alkali stress (0 h, 6 h, and 12 h). A total of 599 differentially co-expressed genes associated with saline–alkali stress were identified. GO and KEGG pathway analysis revealed that DEGs in the leaves were enriched in glutathione metabolism, hydrolase activity, and heme binding following exposure to saline–alkali stress. However, DEGs in the roots were enriched in phenylpropanoid biosynthesis, flavonoid biosynthesis and iron ion binding. We identified hub genes related to superoxide dismutase and Na+, K+ transport using weighted gene co-expression network analysis.Our findings provided new insights into the saline–alkali tolerance of M. niedzwetzkyana at the physiological and molecular levels. this research provides an important genetic resource for identifying genes involved in responses to saline–alkali stress.

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“…MbWRKY5 and MdWRKY30 enhance tolerance to drought and salt in tobacco and Arabidopsis, respectively, by regulating the expression levels of genes associated with stress responses; these genes are involved in alleviating oxidative stress and maintaining the integrity of the cell membrane [49,50]. MdWRKY56 has previously been identified as a positive regulator of drought tolerance, and transgenic apple plants overexpressing the gene encoding this TF have a higher PRO content and antioxidant enzyme activities [51]. However, no key TFs responsive to salt-alkali stress have been reported.…”

Section: Discussionmentioning

confidence: 99%

Physiological Studies and Transcriptomic Analysis Reveal the Mechanism of Saline-Alkali Stress Resistance of Malus sieversii f. niedzwetzkyan

Jiang,

Yang,

Zhou

et al. 2024

Horticulturae

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Malus sieversii f. niedzwetzkyan, a wild species capable of growing on saline-alkali soil in Xinjiang, is the most promising horticultural crop for improving the saline-alkali wasteland. However, the tolerance of M. niedzwetzkyan to saline-alkali stress and the underlying molecular mechanisms remains largely unknown. Here, we conducted a hydroponic experiment in which M. niedzwetzkyana and M. domestica “Royal Gala” seedlings were subjected to 150 mM saline-alkali stress. Physiological data showed that M. niedzwetzkyana had a strong ROS scavenging ability and ion transport ability, and its saline-alkali resistance was higher than that of M. “Royal Gala”. Saline-alkali stress also promoted the synthesis of anthocyanins in M. niedzwetzkyana. Transcriptome analysis was conducted on the leaves and roots of M. niedzwetzkyana at different time points under saline-alkali stress (0 h, 6 h, and 12 h). Transcriptome analysis revealed that saline stress down-regulated most genes involved in the anthocyanin flavonoid synthesis pathway. Transcription levels of genes involved in antioxidant enzyme activity and ion transport were altered. We identified hub genes related to superoxide dismutase as well as Na+ and K+ transport using weighted gene co-expression network analysis. This study elucidated, for the first time at the molecular level, the saline-alkali tolerance of M. niedzwetzkyana, including the complex changes in pathways that regulate reactive oxygen species homeostasis, ion uptake, and anthocyanoside synthesis under saline-alkali stress conditions. This research provides an important genetic resource for identifying genes involved in responses to saline-alkali stress.

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Apple WRKY transcription factor MdWRKY56 positively modulates drought stress tolerance

Cited by 15 publications

References 58 publications

ChaWRKY40 Enhances Drought Tolerance of ‘Dawei’ Hazelnuts by Positively Regulating Proline Synthesis

ChaWRKY40 Enhances Drought Tolerance of ‘Dawei’ Hazelnuts by Positively Regulating Proline Synthesis

Physiological studies and transcriptomic analysis reveal the mechanism of saline–alkali stress resistance of Malus sieversii f. niedzwetzkyan

Physiological Studies and Transcriptomic Analysis Reveal the Mechanism of Saline-Alkali Stress Resistance of Malus sieversii f. niedzwetzkyan

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