Apple‐ and Hop‐Polyphenols Protect Periodontal Ligament Cells Stimulated With Enamel Matrix Derivative From <i>Porphyromonas gingivalis</i>

Inaba, Hiroaki; Tagashira, Motoyuki; Kanda, Tomomasa; Ohno, Takashi; Kawai, Shinji; Amano, Atsuo

doi:10.1902/jop.2005.76.12.2223

Cited by 33 publications

(32 citation statements)

References 47 publications

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“…13) In contrast, EGCg reportedly showed cytotoxicity at a concentration of more than 50 mM (about 23 mg/ml). 26) Our previous study also showed that EGCg caused a significant loss of cellular viability even at a dose of 10 mg/ml.…”

Section: Resultsmentioning

confidence: 94%

“…24) In addition, proteases secreted by P. gingivalis, abundant in its vesicles, are major factors to induce a host inflammatory response, and HBP effectively inhibited protease activity of P. gingivalis. 13) Further investigation is necessary to elucidate the exact pathway to express the PGE 2 -suppressing activity of HBP.…”

Section: Resultsmentioning

confidence: 99%

“…26) Our previous study also showed that EGCg caused a significant loss of cellular viability even at a dose of 10 mg/ml. 13) In addition to the proven safety, HBP was also capable of protecting periodontal cells from bacterial cytotoxicity, 13) as mentioned in the introduction. HBP was previously shown to inhibit cariogenic activity of Streptococcus mutans in vitro, 17) and a recent crossover clinical study showed that a mouthrinse containing HBP successfully reduced dental plaque regrowth in humans.…”

Section: Resultsmentioning

confidence: 99%

“…We previously reported that both hop-and apple-derived polyphenols, hop bract polyphenol (HBP) and apple condensed tannin (ACT) effectively inhibited the protease activity of P. gingivalis, and protected periodontal ligament cells from bacterial cytotoxicity. 13) Their protective abilities were found to be greater than EGCg. In the present study, we examined whether HBP and ACT can inhibit PGE 2 production by HGE cells stimulated with P. gingivalis vesicles in order to explore an alternative to periodontal therapy.…”

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confidence: 95%

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Identification of Hop Polyphenolic Components Which Inhibit Prostaglandin E2 Production by Gingival Epithelial Cells Stimulated with Periodontal Pathogen

Inaba

Tagashira

Honma

et al. 2008

Biological & Pharmaceutical Bulletin

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Chronic marginal periodontitis is a destructive inflammatory disease caused by an imbalance between bacterial virulence and host defense ability, resulting in eventual tooth exfoliation. Porphyromonas gingivalis, a major periodontal pathogen, triggers a series of cellular inflammatory responses including the production of prostaglandin E 2 (PGE 2 ), which causes periodontal destruction; thus, anti-inflammatory reagents are considered beneficial for periodontal therapy. In the present study, we examined whether hop-and apple-derived polyphenols (HBP and ACT, respectively) inhibit PGE 2 production by human gingival epithelial (HGE) cells stimulated with P. gingivalis components. HGE cells were stimulated with P. gingivalis membrane vesicles, and the effects of HBP, ACT and epigallocatechin gallate (EGCg) on PGE 2 production by HGE cells were evaluated using an enzyme-linked immunosorbent assay. HBP and EGCg significantly inhibited PGE 2 production, whereas ACT did not. , were found to be elements which significantly inhibited cellular PGE 2 production. These results suggest that HBP is a potent inhibitor of cellular PGE 2 production induced by P. gingivalis, and HBP may be useful for the prevention and attenuation of periodontitis.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 95%

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Identification of Hop Polyphenolic Components Which Inhibit Prostaglandin E2 Production by Gingival Epithelial Cells Stimulated with Periodontal Pathogen

Inaba

Tagashira

Honma

et al. 2008

Biological & Pharmaceutical Bulletin

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“…A few polyphenols derived from plants such as green tea (Camellia y To whom correspondence should be addressed. Fax: +81-256-52-6634; E-mail: ootsubo@ari.pref.niigata.jp sinensis), 27) cranberry (Vaccinium macrocarpon), 28,29) hop (Humulus lupulus L.) bract and apple (Malus domestica) 30) have been reported to inhibit both the enzymatic activities of gingipains and the biofilm formation and cytotoxicity by P. gingivalis. We have already discovered that the protein fraction of rice (Oryza sativa L. japonica) grain inhibited Rgp and, more weakly, Kgp.…”

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confidence: 99%

Potential Value of a Rice Protein Extract, Containing Proteinaceous Inhibitors against Cysteine Proteinases fromPorphyromonas gingivalis, for Managing Periodontal Diseases

Taiyoji¹,

Yamanaka²,

Tsuno³

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Arg-specific gingipain (Rgp) is a major pathogenic determinant of Porphyromonas gingivalis which is a major pathogen in periodontal disease. We prepared protein extracts with Rgp-inhibitory activity from polished rice (Oryza sativa) and evaluated the effects of these extracts on the growth and pathogenicity of P. gingivalis. The extracts inhibited the proteolytic degradation of human proteins by P. gingivalis proteinases, and repressed the growth and homotypic biofilm formation of P. gingivalis. The disruption of adhesion of epithelial cells by P. gingivalis was also restricted by the rice protein extracts. Our results suggested that the rice protein extracts suppressed the pathogenicity and growth of P. gingivalis by inhibiting the bacterial proteinase activities, implying that the Rgp-inhibitory proteins prepared from rice may be potentially valuable as nutraceutical agents for preventing periodontal diseases.

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Healthy properties of proanthocyanidins

et al. 2010

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Proanthocyanidins, also named condensed tannins, are the result of flavanols condensation. Oligomers and polymers of proanthocyanidins can widely be found in the plant kingdom, as in fruits and berries, seeds, flowers, and leaves. They have a putative role as antioxidants, and they affect the inflammatory process via calcium-dependent release of nitric oxide and protect against H(2)O(2)-induced lipid peroxidation. They also demonstrated a role in cardiovascular diseases via vessel relaxation and LDL oxidation inhibition. These condensed tannins have also shown activities that improve diabetic complications, such as neuropathy, retinopathy, or nephropathy, including a decrease in serum glucose and advanced glycation end products. Furthermore, proanthocyanidins have evidenced anticancer properties by mitigating tumor development through induction of apoptosis or inhibition of cell proliferation. Finally, they are able to produce antiadhesive actions against bacteria in urinary and dental infections, including Escherichia coli and Streptococcus mutans. Hence, proanthocyanidins are considered as beneficial molecules in preventing or treating many diseases and pathological conditions. Therefore, finding out more about condensed tannins bioavailability, and understanding the regulatory genes and pathways involved in their effects should be aimed in future research.

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Apple‐ and Hop‐Polyphenols Protect Periodontal Ligament Cells Stimulated With Enamel Matrix Derivative From Porphyromonas gingivalis

Abstract: These results suggest that AP, ACT, AP, HBP, and HMW-HBP protect EMD-stimulated PDL cells from P. gingivalis and may be therapeutically useful supplements for EMD therapy.

Cited by 33 publications

References 47 publications

Identification of Hop Polyphenolic Components Which Inhibit Prostaglandin E2 Production by Gingival Epithelial Cells Stimulated with Periodontal Pathogen

Identification of Hop Polyphenolic Components Which Inhibit Prostaglandin E2 Production by Gingival Epithelial Cells Stimulated with Periodontal Pathogen

Potential Value of a Rice Protein Extract, Containing Proteinaceous Inhibitors against Cysteine Proteinases fromPorphyromonas gingivalis, for Managing Periodontal Diseases

Healthy properties of proanthocyanidins

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