Appearance of Nitrite Reducing Activity of Cytochrome<i>c</i>upon Heat Denaturation

Yamada, Seiji; Suruga, Kohei; Ogawa, Masahiro; Hama, Toshiyuki; Satoh, Tadashi; Kawachi, Ryu; Nishio, Takeshi; Oku, Tatsuo

doi:10.1271/bbb.66.2044

Cited by 11 publications

(9 citation statements)

References 18 publications

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“…The residual activity is likely connected with the fact that in the denatured protein five coordinated heme c exposed to the aqueous medium. Similar nitrite reductase activity was detected in cytochrome c subjected to denat uration or limited proteolysis [37]. Characterization of the oligomeric composition of TvNiR by small angle X ray scattering (SAXS).…”

Section: Thermostability Of Tvnirmentioning

confidence: 69%

10.1007/s10541-008-2007-8

2000

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Section: Thermostability Of Tvnirmentioning

confidence: 69%

10.1007/s10541-008-2007-8

2000

CrossRef Listing of Deleted DOIs

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“…The amount of NO 2 -was deter mined by the Griess reaction with SA and NEDA. 16 Samples of the reaction mixture were thawn, and SA and NEDA solutions were added. The reaction was completed in 50 min at 20 °C.…”

Section: Methodsmentioning

confidence: 99%

Reduction of nitrates, the no donors, by hemoglobin in the presence of cysteine

et al. 2007

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Hemoglobin (Hb) reduces 3,3 bis(nitroxymethyl)oxetane (NMO) only in the presence of cysteine (Cys) via intermediate cysteine thionitrate. The kinetics of the reaction of NMO with Cys and the kinetics and mechanism of formation of NO in the ternary system Hb-NMO-Cys were studied. The formation rate of Hb-NO in the ternary system is higher than that of Hb-NO in the reaction of Hb only with NO 2 -generated in the binary system NMO-Cys. The second order rate constants of the main reaction steps in the system Hb-NMO-Cys were estimated by simulating the kinetics of the reactions with a system of equations taking into account equilibria between all components of the reaction mixture. Hemoglobin reduces cysteine thionitrate, the intermediate in the reaction of NMO with Cys, to NO.

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“…The potentials were measured with a HORIBA F-13 pH meter equipped with an ORP electrode, and the optical spectra were monitored throughout the titration on a HITACHI U3310 spectrophotometer. The redox mediators were used to stabilize the solution redox potential as described in Yamada et al (5). The redox data were analyzed with a theoretical curve based on the Nernst equation (n ) 1): E ) E°+ (RT/nF) ln([ferric]/[ferrous]).…”

Section: Preparation Of Hemoproteinsmentioning

confidence: 99%

“…Yamada et al reported that heat-treated equine heart Cyt c reduces NO 2to ammonia (NH 4 + ) (5). This unexpected phenomenon, in which a new catalytic activity appears after heat treatment, is contrary to those observed in well-known biological molecules, which are generally inactivated by heat.…”

Section: Introductionmentioning

confidence: 96%

Radiation-Induced Enhancement of Nitrite Reducing Activity of Cytochrome c

Suruga

Nagasawa

Yamada

et al. 2003

J. Agric. Food Chem.

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Commercial cytochrome c (Cyt c) was irradiated with Co-60 gamma-rays in the dose range of up to 3.0 kGy to investigate the enhancement of the nitrite reducing activity of Cyt c. The optimum irradiation dose to induce nitrite reducing activity for 30 muM Cyt c solution was 1.0 kGy under an O(2) atmosphere. The nitrite reducing activity of Cyt c irradiated at this dose was approximately 45-fold that of unirradiated Cyt c and ca. 1.2-fold that of nitrite reductase. The irradiation treatment resulted in unfolding of the peptide chain, exposure of the heme group, oxidation of methionine to methionine sulfoxide, dissociation of the sixth ligand (Met), and occurrence of autoxidation in Cyt c. Sepharose-immobilized irradiated Cyt c had a similar activity to that in solution. The resin retained the activity after five uses even after 1 year of storage. The irradiated Cyt c will be able to be used as a substitute for nitrite reductase.

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Appearance of Nitrite Reducing Activity of Cytochromecupon Heat Denaturation

Cited by 11 publications

References 18 publications

10.1007/s10541-008-2007-8

10.1007/s10541-008-2007-8

Reduction of nitrates, the no donors, by hemoglobin in the presence of cysteine

Radiation-Induced Enhancement of Nitrite Reducing Activity of Cytochrome c

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