Appearance of iron-labeled blood mononuclear cells in electron microscopy

Abstract

It is necessary to note that although there is a considerable number (several thousands) of publications regarding the use and role (presupposed) of bone marrow mononuclear cells in the therapy of different diseases (Futterman and Lemberg, 2004;Uzan, 2004) and reparation of posttraumatic lesions, we have been unable find information on their morphology. References such as "identification of morphology, size and shape is difficult" were mentioned in some papers (Dalrup-Link et al., 2003). Therefore the description of the morphology of this cell type is the aim of our study. These cells will be put to morphometric measurement, and their distribution whithin different regions of experimental myocardial infarction in the next phase of the experiment. MATERIAL AND METHODS Animal modelRabbits (Hy-Plus, weight 2.5 to 3.5 kg) were used as an experimental animal model. Experimental protocol was approved by the Ethics Committee of University of Veterinary Medicine, Brno. Bone marrow collectionMononuclear cells were harvested from rabbit bone marrow on day -14, before the ligation of the coronary artery. Bone marrow was obtained by multiple aspiration from iliac bone into heparin flushed syringes under general anesthesia. Samples were collected into tubes containing heparin and 0.9%NaCl (1:1) under sterile conditions. A sufficient amount of bone marrow was collected in order to obtain the required numbers of enriched cells (approximately 5-10 ml). Selection of mononuclear cellsMononuclear cells were enriched from bone marrow by density gradient centrifugation. Bone marrow was diluted with Hanks/4% albumin solution (1:1) and overlaid on a sterile density medium ABSTRACT: Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM (transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes. Appearance of iron-labeled blood mononuclear cells in electron microscopy

Fatty Acid Profile and Antimicrobial Activity of Oils Extracted from Seeds of Oleaginous Plant Species

“…By interpreting MIC, Skrivanova et al (2006) observed inhibitory effect of caprylic and capric acid, in their respective concentrations of 21 mM and 29 mM, against E. coli and Salmonella sp. [27] Nair (2005) contained that monocaprine, glyceride of capric acid, effectively eliminated E. coli when added at high concentrations (50 mM) in milk and apple juices. [28][29] Unlike these studies, Preuss et al (2005) in a study on the bactericidal effect of monolaurine in bacteria, it did not find antimicrobial activity of this glyceride in E. coli and Klebsiella pneumoniae, while Helicobacter pylori was sensitive.…”