Appearance of autoantigens during gonad maturation in the rainbow trout <i>(Salmo gairdneri)</i>

Secombes, Christopher J.; Lewis, Andrew D.; Needham, E A; Laird, Lindsay M.; Priede, Imants George

doi:10.1002/jez.1402330311

Cited by 14 publications

(5 citation statements)

References 19 publications

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“…Such a barrier system is present in fish (Abraham et al, 1980;Marcaillou and Szollosi, 1980) and testosterone has been shown to regulate its function in vertebrates (Kevorkov et ul., 1983). Specific antigens are presented on the spermatids and spermatozoons surfaces (Secombes et al, 1985;Lou et al, 1989;Beck et al, 1992). High androgen levels might suppress the immune responses, including autoimmunity, and therefore prevent the ripe germ cells from destruction during the natural breeding season.…”

Section: Discussionmentioning

confidence: 99%

Effects of the androgen preparation "Testoenatum" on reproductive function in males of Atlantic salmon and several species of whitefish

Christoforov

Murza²

1996

Aquat. Living Resour.

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Atlantic salmon, Salrno salar, and several species of whitefish, Coregonus nasus, C. peled, C. luvaretus baeri, males were treated with a long-acting medical androgen preparation -"Testoenatum". This preparation contains 24 mg of testosterone enanthate and 111 mg of testosterone propionate in 1 ml peach oil solution. Multiple (2-5) or single intraperitoneal injections of a standard dose (0.1 mVkg fish body weight) were used. Treatment performed shortly before the beginning or within the breeding season resulted in a signiticant stimulation of spermiation, improvement of sperm parameters and extention of the gonad functional maturity period for 1-4 months. Low water temperatures (0.5-l.O°C) seem to be essential to obtain a long-lasting effect after one injection. The histology of the pituitary gland suggests that the gonadotropic cells were activated through a positive feedback mechanism. Testoenatum suppressed Sertoli cells' phagocytary activity.

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Section: Discussionmentioning

confidence: 99%

Effects of the androgen preparation "Testoenatum" on reproductive function in males of Atlantic salmon and several species of whitefish

Christoforov

Murza²

1996

Aquat. Living Resour.

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“…In mammals, interactions of the spermatozoa with the seminal fluid would seem to play a major role in the surface membrane changes which accompany epididymal sperm maturation (Barrios et al., 2000; Cardozo et al., 2006; Muiño‐Blanco et al., 2008). In teleosts, several auto‐antigens appear on germ cells during spermiogenesis (Secombes et al., 1985; Lou and Takahashi, 1991) and have been related to several germ cell‐specific functions during spermiogenesis, sperm maturation and fertilisation.…”

Section: Introductionmentioning

confidence: 99%

Protein profile study in European eel (Anguilla anguilla) seminal plasma and its correlation with sperm quality

Peñaranda

Marco‐Jiménez

Pérez

et al. 2010

Journal of Applied Ichthyology

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Along with sperm quality parameters, the protein profile of European eel seminal plasma was analyzed during induced spermiation (n = 56 samples). Motility, Percentage of live cells, spermatozoa head morphometry and concentration showed low values during the initial weeks of spermiation and maintained high levels throughout the rest of the experiment. The protein profile gradient by SDS-PAGE (4-15%) registered four important electrophoretic bands around 80, 40, 26 and 12 KDa. Three of them showed significant differences in concentration during treatment (80, 40 and 12 KDa), and all of them showed the highest value on the 8th week. Both 80 and 12 KDa bands increased until the 8th week, followed by a progressive decline. One possible explanation for these profiles is that, in the first weeks of treatment, proteins originated from blood plasma are accumulated in the seminal plasma, and from the 8th week some of these proteins are incorporated into the spermatic membranes. The 40 KDa protein band also increased during the first 8 weeks, but maintained high concentrations in the seminal plasma for the rest of the experiment. One result confirms the theory that the presence of proteins in the seminal plasma having a molecular weight lower than 50 KDa increased spermatozoa motility, since the 40 KDa band displayed significantly higher values coinciding with the high percentages of spermatozoa motility. Seminal plasma proteins seem to have an important role in spermatogenesis and spermatozoa movement, but further studies are necessary to discover the identity of these proteins and their precise functions.

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“…Several proteins expressed during spermiogenesis are known to play important roles in fertilization, such as sperm‐zona binding 6–9 and sperm‐egg plasma membrane binding 10,11 . Also, in teleosts, several proteins are synthesized and expressed in germ cells during spermiogenesis, 12–14 suggesting that these proteins are related to several germ‐cell‐specific functions during spermiogenesis and fertilization as they are in mammals. However, in teleosts, few studies have been conducted to investigate the protein synthesis itself in testis.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning of an elongation factor 1alpha and its mRNA localization in testis of the Nile tilapia Oreochromis niloticus

Mochida

Matsubara

2002

Fisheries Sci

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During spermatogenesis, many proteins are synthesized in the testis prior to the completion of sperm maturation. Many components are involved in the testicular protein synthesis and one of the components that is implicated in the polypeptide chain elongation is elongation factor 1α. In the present study, the molecular cloning of elongation factor 1α (EF‐1α) was conducted from a testis cDNA library of the Nile tilapia. The cDNA for tilapia EF‐1α (tEF‐1α) contains a complete open reading frame encoding 462 amino acids. The predicted amino acid sequence of EF‐1α shows an approximate 90% similarity to those identified in other teleost fish, such as medaka, sea bream and zebrafish. Northern blotting revealed that the gene is expressed in all of the examined tissues and in ovulated eggs. The results of in situ hybridization indicate that the gene is expressed specifically in Leydig cells in testis, suggesting the involvement of EF‐1α as an actin‐binding protein in the cluster formation of Leydig cells. In the ovary, the gene is expressed in the perinucleolus stage of oocytes, suggesting that EF‐1α is also implicated in oocyte growth.

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Appearance of autoantigens during gonad maturation in the rainbow trout (Salmo gairdneri)

Cited by 14 publications

References 19 publications

Effects of the androgen preparation "Testoenatum" on reproductive function in males of Atlantic salmon and several species of whitefish

Effects of the androgen preparation "Testoenatum" on reproductive function in males of Atlantic salmon and several species of whitefish

Protein profile study in European eel (Anguilla anguilla) seminal plasma and its correlation with sperm quality

Molecular cloning of an elongation factor 1alpha and its mRNA localization in testis of the Nile tilapia Oreochromis niloticus

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