Apparent molar heat capacities of phospholipids in aqueous dispersion. Effects of chain length and head group structure

Blume, Alfred

doi:10.1021/bi00292a027

Cited by 182 publications

(120 citation statements)

References 53 publications

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“…The following order of π c values for different lipids was found: 1,1′-DMBMP > 3,1′-DMBMP > DPPG > DMPE. Under similar solvent conditions, it is reported the main phase transition temperatures of DPPG and DMPE as determined by DSC are ∼41.3°C (44, 53, 66) and >49.5°C (40,67,68), respectively. Therefore, the order of π c values given above reflects, at least in part, the order of the main phase transition temperatures of these lipids.…”

Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 75%

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

et al. 2006

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Bis(monoacylglycero)phosphate (BMP) reveals an unusual sn-1,sn-1′ stereoconfiguration of glycerophosphate. We synthesized sn- (3-myristoyl-2-hydroxy)glycerol-1-phospho-sn-1′-(3′-myristoyl-2′-hydroxy)glycerol (1,1′-DMBMP) and characterized the thermotropic phase behavior and membrane structure, in comparison with those of the corresponding sn-3:sn-1′ stereoisomer (3,1′-DMBMP), by means of differential scanning calorimetry (DSC), small-and wide-angle X-ray scattering (SAXS and WAXS, respectively), pressure-area (π-A) isotherms, epifluorescence microscopy of monolayers, and molecular dynamics (MD) simulations. In DSC, these lipids exhibited weakly energetic broad peaks with an onset temperature of 9°C for 1,1′-DMBMP and 18°C for 3,1′-DMBMP. In addition, a highly cooperative, strongly energetic transition peak was observed at ∼40°C for 1,1′-DMBMP and ∼42°C for 3,1′-DMBMP. These results are supported by the observation that 1,1′-DMBMP exhibited a larger phase transition pressure (π c ) than 3,1′-DMBMP. Small-and wide-angle X-ray scattering measurements identified these small and large energetic transitions as a quasi-crystalline (L c1 )-quasi-crystalline with different tilt angle (L c2 ) phase transition and an L c2 -L R main phase transition, respectively. X-ray measurements also revealed that these DMBMPs undergo an unbinding at the main phase transition temperature. The MD simulations estimated stronger hydrogen bonding formation in the 3,1′-DMBMP membrane than in 1,1′-DMBMP, supporting the experimental data.Bis(monoacylglycero)phosphate, also known as lysobisphosphatidic acid (LBPA), was first described in pig and rabbit pulmonary alveolar macrophages by Body and Gray (1). BMPs comprise less than 1% of the total phospholipids in most mammalian cells (2, 3). However, the content is increased to 15% in late endosomes (4). In the specific internal membrane domains of this organelle, this lipid comprises more than 70% of the total phospholipids (5).Because of the high level of accumulation in late endosomes, BMP is assumed to be important for the structural and functional integrity of the organelle. Indeed, a series of experiments has demonstrated that agents that perturb BMP induce alterations in the sorting and trafficking of proteins and/or the receptor in late endosomes accompanied by structural changes of the late edonsome organelle (4, 6) along with abnormal accumulation of cholesterol (7). Moreover, BMP is found to be essential for the activator-stimulated hydrolysis of ganglioside G M1 (8) and G M2 (9) and for the hydrolysis of ceramide by acid ceramidase (10).BMP has unique structural characteristics with various fatty acid compositions depending on the source. Whereas all other mammalian phospholipids display a sn-3-glycerophosphate stereoconfiguration, BMP possesses an sn-1:sn-1′ structure and one acyl group attached to each of the glycerol moieties (11-13). An sn-3:sn-1′ isomer has also been reported for BMP in BHK cells (14). However, the sn-3:sn-1′ isomer in mammalian cells is postulated to be...

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Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 75%

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

et al. 2006

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“…It has been suggested that cholesterol exerts its influence by the hydration of the -OH group, ordering the water near the membrane surface [22]. Moreover, cholesterol decreases lipid chain mobility, thus reducing structural defects and therefore the space in wlfich water molecules can be accommodated [23]. Kao et al [24] have shown that the presence of cholesterol reduces the apparent polarity of the hydrocarbon region of the phospholipid bilayer.…”

Section: Discussionmentioning

confidence: 99%

Effect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions

Fiorini

Gratton

Curatola

1989

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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“…The transition temperature of DDPC to its liquid crystalline phase is 25.6 ˚C [13], which is slightly higher than DMPC (24.0 ˚C) [14]. The phase behavior of a DMPC/DDPC mixed system has also been examined previously, and no phase separation was detected in a differential scanning calorimetric study around the present DDPC content [11].…”

Section: Immobilization and Lipid Composition Of Liposomesmentioning

confidence: 63%

“…To distinguish those liposomes first immobilized onto the gel from the newly added ones, the latter were radio-labeled with [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]cholesteryl oleate. Upon the coincubation, the radioactivity in the supernatant started to disappear as shown in Figure 3A.…”

Section: Interaction Of Liposome-immobilizing Gels With Fresh Liposomesmentioning

confidence: 99%

Immobilization of liposomes on hydrophobically modified polymer gel particles in batch mode interaction

Okumura

Mizushima

Fujinaga

et al. 2007

Colloids and Surfaces B: Biointerfaces

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Immobilization of liposomal phospholipids onto Sephacryl S-1000 gels that were chemically conjugated with hydrophobic alkyl moieties, octyl, dodecyl and hexadecyl, was examined in batch mode interaction. Compared with the octyl gel, the dodecyl and the hexadecyl gels were found to immobilize the 3-4 times more phospholipids with the less hydrophobic moieties. The encapsulation of a water-soluble marker, with other evidences, suggests that the majority of the immobilized phospholipids maintained liposomal morphology. As the lipid of the interacting liposomes, egg yolk phosphatidylcholine (eggPC), 1,2-dimyristoylphosphatidylcholine (DMPC) and a mixture of DMPC and 1,2-dimyristamido-1,2-deoxyphosphatidylcholine were examined.At 22 ˚C, DMPC liposomes showed higher extent of immobilization than at 37 ˚C but not eggPC liposomes, suggesting that the phase of liposomal membrane could have influence on the immobilization. Exchange between the immobilized liposomes and free ones was found be small less than 3%. The gel that had been first interacted with liposomes to apparent saturation could further immobilize the newly added liposomes. The rate of this second immobilization was similar to that of the slow adsorption process; the both could be based on the same mechanism, possibly involving rearrangement of the immobilized liposomes on the gel as proposed by Lundahl.As had been observed in the flow mode, the immobilization had preference for smaller liposomes.In application of the system in batch mode, the size distributions of the immobilized liposomes and of those left in the supernatant may differ from that of the originally added liposomes.3

show abstract

Apparent molar heat capacities of phospholipids in aqueous dispersion. Effects of chain length and head group structure

Cited by 182 publications

References 53 publications

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

Effect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions

Immobilization of liposomes on hydrophobically modified polymer gel particles in batch mode interaction

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