Apparent absence of viruses in most symptomless field‐grown sweet potato in Uganda

Gibson, R. W.; Mwanga, R.O.M.; Kasule, S.; Mpembe, I.; Carey, Edward E.

doi:10.1111/j.1744-7348.1997.tb07676.x

Cited by 80 publications

(67 citation statements)

References 15 publications

(12 reference statements)

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“…Field observations at MUARIK indicated that each plant could be infested by over 200 whiteflies and this increases the likelihood of being infected with SPCSV. However, aphids, the vectors of SPFMV rarely colonise the sweetpotato plants in Uganda (Gibson et al, 1997). This could explain why SPFMV is less common in the healthy looking vines.…”

Section: Discussionmentioning

confidence: 99%

Detection and elimination of sweetpotato viruses

Rukarwa

Mashingaidze²,

Kyamanywa³

et al. 2011

African Crop Science Journal

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In sub-Saharan Africa, sweetpotato (Impomoea batatas L.) production is greatly constrained by sweetpotato virus disease (SPVD) complex. This study was conducted to assess the incidence of viruses in healthy-looking sweetpotato in Uganda and to optimise modern technologies for virus diagnosis. A collection of healthy-looking sweetpotato vines from central Uganda were serologically assayed for sweetpotato viruses and the positive samples were confirmed by RT-PCR. A multiplex RT-PCR assay was optimised for simultaneous detection of Sweet potato chlorotic stunt virus (SPCSV), Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV). The use of in vitro thermotherapy was also investigated as a means of eliminating sweetpotato viruses. Four viruses namely SPCSV, SPFMV, SPMMV and SPCFV were detected mostly as single infections in the healthy looking plants. SPCSV (70. 6%) recorded highest incidence followed by co-infection of SPFMV and SPCSV (8.3%). Based on shoot survival and effectiveness of virus elimination, the best results were obtained by exposing plantlets to daily temperature regime of 32 RÉSUMÉEn Afrique sub saharienne, la production de la patate douce (Impomoea batatas L.) est grandement affectée par un complexe de maladies de virus (SPVD). Cette étude était conduite pour évaluer l'incidence maladie des virus sur des boutures apparemment saines de la patate douce en Ouganda et optimiser les technologies pour diagnostic de virus. Des boutures apparemment saines de patate douce collectées au centre de l'Ouganda étaient sérologiquement testées et les échantillons infectés étaient confirmés par RT-PCR. Un essai multiplexe RT-PCR était optimisé pour la detection simultanée du virus du rabougrissement chlorotique de la patate douce (SPCSV), le virus de la marbrure duveteuse de la patate (SPSMV) et le virus de marbrure modérée de la patate douce (SPMMV). L'usage de la thermothérapie in vitro était aussi testé comme moyen d'élimination des virus de la patate. Quatre virus dont SPCSV, SPFMV, SPMMV et SPCFV étaient detectés surtout comme seules infections des plantes apparemment saines. Le SPCSV (70. 6%) avait présenté une incidence élevée, suivi de SPFMV et SPCSV dont le niveau d'infection était le même (8.3%). Basé sur la survie des pousses et l'efficacité de l'élimination de virus, les meilleurs résultats étaient obtenus en exposant les plantules à un regime de température de 32 o C pendant 8 heures sous obscurité et 36 o C pendant 16 heures sous lumière durant quatre semaines. La culture du bout du méristème combinée à la thermothérapie a perimis l'élimination de SPFMV et SPMMV dans 77 % des plants qui étaient au départ infectés avec des virus respectifs. Par ailleurs, l'élimination de SPCSV avait échoué.

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Section: Discussionmentioning

confidence: 99%

Detection and elimination of sweetpotato viruses

Rukarwa

Mashingaidze²,

Kyamanywa³

et al. 2011

African Crop Science Journal

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“…Studies in Uganda indicate no beneWt of using pathogen-tested plants when compared with farm-derived plants of the same cultivars in the region (Gibson et al 1997;Carey et al 1999). Thus, plant resistance is important to SPVD control (Karyeija et al 1998b;Carey et al 1999).…”

Section: Introductionmentioning

confidence: 96%

“…Sweet potato virus disease (SPVD), a result of coinfection of whiteXy transmitted Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus, family Closteroviridae) and the aphid-transmitted Sweet potato feathery mottle virus (SPFMV, genus Potyvirus, family Potyviridae), is the most destructive disease of sweet potato world-wide and especially in East Africa (Geddes 1990;Gibson et al 1997;Carey et al 1999;Karyeija et al 2000;Gutierrez et al 2003). The disease is characterized by chlorosis, small, deformed leaves, and severe stunting and can reduce yields of infected plants by over 90% (Gibson et al 1998;Gutierrez et al 2003).…”

Section: Introductionmentioning

confidence: 98%

Identification of molecular markers associated with sweet potato resistance to sweet potato virus disease in Kenya

2007

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Sweet potato virus disease (SPVD), a result of the co-infection of whiteXy transmitted Sweet potato chlorotic stunt virus (genus Crinivirus, family Closteroviridae) and the aphid transmitted Sweet potato feathery mottle virus (genus Potyvirus, family Potyviridae), is the most destructive disease of sweet potato in East Africa. A study was conducted to establish if genotypes identiWed as resistant or susceptible to SPVD in Kenya could be distinguished using molecular markers. A total of 47 unrelated sweet potato genotypes were selected from germplasm collections and classiWed into two phenotypic groups as resistant or susceptible to SPVD. Genotype selection was based on disease severity or days to symptom development in plants following graft inoculation.AmpliWed fragment length polymorphism (AFLP) marker proWles were generated for each individual and used in association studies to identify markers suitable for classifying the two pre-deWned phenotypic groups. Analysis of molecular variance showed signiWcant (P < 0.002) variation between the two groups using 206 polymorphic AFLP markers. Discriminant analysis and logistic regression statistical methods were used to select informative markers, and to develop models that would classify the two phenotypic groups. A training set of 30 genotypes consisting of 15 resistant and 15 susceptible were used to develop classiWcation models. The remaining 17 genotypes were used as a test set. Four markers, which gave 100% correct classiWcation of the training set and 94% correct classiWcation of the test set, were selected by both statistical methods.

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“…Farmer's slips was produced from the farm produced seed tuber over 5 years. (Gibson et al, 1997;Ngevea1 and Bouwkamp, 1991;Untiveros et al, 2007). 한편 무병묘 재배에서도 바이 러스 재감염으로 인하여 품종 고유의 수량 및 품질 특성을 오랫동안 유지하는 것은 어렵다.…”

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Growth Characteristics and Yield of Sweet Potato Cultivars between Virus-free and Farmer's Slips in Late Season Cultivation

유경란¹,

이승엽²

2013

Korean Journal of Crop Science

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This work was conducted to obtain some information about stable production of high quality seed-tubers in the late season cultivation of virus-free sweet potato [Ipomoea batatas (L.) Lam.]. Growth characteristics and storage root yield between virus-free and farmer's slips in 9 cultivars were investigated using black-film vinyl mulching cultivation with 75×25 cm planting density on July 10. At 30 days after planting, vine length, vine diameter, number of node, and number of branch in virus-free slips were significantly increased than those in farmer's slips. The vine growth was significantly different among cultivars, and vine elongation was excellent in 'Kogeonmi', 'Shincheonmi', 'Shinhwangmi', 'Shinyulmi', and 'Yeonhwangmi' compared to the other cultivars. At 110 days after planting, vine length, vine diameter, number of node, number of branch, and fresh weight were significantly different among cultivars, but no significant differences between virus-free and farmer's slips were seen except number of node. Total yield in virus-free slips was increased by 12-49% among cultivars than that in farmer's slips. The mean yields between virus-free and farmer's slips were 1,625 kg/10a and 1,230 kg/10a, respectively, and it was significantly different between virus-free and farmer's slips. Percentage of marketable storage root in virus-free slips was 65.6%, and it was significantly higher than 57.8% in farmer's slips. Marketable yields (40 g≤) between virus-free and farmer's slips were 1,067 kg/10a and 710 kg/10a, respectively. Marketable yield in 'Shincheonmi', 'Shinyulmi' and 'Shinzami' was more than 1,300 kg/10a, and these cultivars showed to be highly adaptable for the late-season cultivation among 9 tested cultivars.

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Apparent absence of viruses in most symptomless field‐grown sweet potato in Uganda

Cited by 80 publications

References 15 publications

Detection and elimination of sweetpotato viruses

Detection and elimination of sweetpotato viruses

Identification of molecular markers associated with sweet potato resistance to sweet potato virus disease in Kenya

Growth Characteristics and Yield of Sweet Potato Cultivars between Virus-free and Farmer's Slips in Late Season Cultivation

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