We present the first calculations of intrinsic D 2 over H 2 (D 2 /H 2 ) selectivity in well-defined crystalline nanoporous material, namely, NaX zeolite. Feynman path integrals with realistic force field (Kowalczyk, P.; Gauden, P. A.; Terzyk, A. P.; Pantatosaki, E.; Papadopoulos, G. K. J. Chem. Theory Comput. 2013, 9, 2922−2929 are used to calculate zero-and finite-pressure adsorption D 2 /H 2 selectivity on NaX at 77 K. The kinetic selectivity is computed by classical molecular dynamics with Feynman−Hibbs quantum effective potentials. We found that within Henry's law region the intrinsic D 2 /H 2 selectivity of NaX is only ∼1.22−1.31. On the contrary, the theoretical and experimental adsorption D 2 /H 2 selectivities on NaX are 1.49 and 1.6, respectively. A reduction of adsorption selectivity by approximately 13−19% is explained by faster self-diffusion of H 2 than D 2 molecules in NaX crystal (i.e., normal isotope kinetic effect on self-diffusion). Our results clearly demonstrate the interplay between adsorption and kinetic factors that may have important implications for separation of H 2 /D 2 mixtures using permselective nanoporous membranes.