The variability in climatic conditions during product distribution, especially across large distances, can be significant and is well known to affect the mechanical properties of many packaging materials. As the use of environmentally friendly materials, such as paperboard and bio-cushions, increases, the challenge associated with overcoming the effects of extremes in temperature and relative humidity in the distribution chain becomes critical. To date, in the case of paperboard boxes, this is dealt with by accounting for the loss of static (compression) strength with increasing relative humidity. However, no method exists to address the dynamic loads induced by vehicle shocks and vibrations especially for configurations that involve stacked boxes and where the vibration intensity within the stack is influenced by the dynamic characteristic of the boxes themselves. In such scenarios, it is the variation in the stiffness of the box as a function of environmental conditions and dynamic load that needs to be established. This paper describes the evaluation of the fatigue resistance of paperboard boxes subjected to random excitation and compares the results with those obtained from quasi-static compression tests under various environmental conditions. Results reveal a lack of correlation between the static and dynamic tests. This finding is attributed to changes in internal damping of the paperboard box samples which, when reduced, results in increased dynamic force. The paper concludes that static testing alone is insufficient to establish the fatigue resistance of stacked packaging subject to variations in climatic conditions.