Apparatus for Conditioning Unlimited Quantities of Finished Waters for Enteric Virus Detection

Abstract

An apparatus has been developed, constructed, and tested for conditioning unlimited quantities of finished waters for enteric virus detection.

Enteric virus and indicator bacteria levels in a water treatment system modified to reduce trihalomethane production

“…Four separate virus concentrator setups, similar to those described by Hill et al (11), were used to perform the sampling. Each concentrator was mounted on a wooden frame, and each had an in-line pH probe.…”

Concentration of Bacteriophages from Natural Waters

“…In the last decade considerable anxiety has been caused by contamination of potable waters by animal viruses and this has led to the development of methods for detecting small numbers of viruses in large volumes of drinking water. With few exceptions these methods have been specifically designed to detect acid-resistant enteroviruses, usually poliovirus, and generally use adsorption to various filters at pH 3-0-3-5 followed by elution of the viruses in a small volume of buffer-pH 10-5-1 1.5 (Wallis, Henderson & Melnick 1972;Sobsey et al 1973; Hill et al 1974; Jakubowski et al 1974;Jakubowski et al 1975;Farrah et al 1976; Hill et al 1976; Katzenelson et al 1976; Farrah et a2. 19776).…”

Detection of virus in water: sensitivity of the tentative standard method for drinking water

“…The LSc2ab strain of poliovirus type 1 was used throughout as the model virus for the quantitative sensitivity studies. The poliovirus stock was filtered by a technique described previously (4) to remove viral aggregates and was considered to be monodispersed. The filtered poliovirus stock was prepared as pools in nutrient broth containing approximately 50 to 100 plaque-forming units (PFU) per ml and stored in 3to 10-ml quantities at -80 C until used.…”

“…Characteristics of the finished water during the period of the experiments are shown in Table 1. The water was conditioned for pH control and chlorine neutralization continuously during the experimental runs by use of an apparatus modified after Hill et al (4) (Fig. 1).…”