Apoptotic response of uveal melanoma cells upon treatment with chelidonine, sanguinarine and chelerythrine

Kemény-Beke, Ádám; Aradi, J.; Damjanovich, Judit; Beck, Zoltán; Facskó, Andrea; Berta, András; Bodnár, Andrea

doi:10.1016/j.canlet.2005.05.037

Cited by 94 publications

(75 citation statements)

References 23 publications

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“…38 ). A similar effect of both alkaloids, SA and CHE, was found on a primary human uveal melanoma OCM-1 cell line 39 . At lower concentrations (2.7 and 10 μM) of SA or CHE, apoptosis was found in the cells, whereas for higher doses of alkaloids (20 μM) necrosis was the dominant form of cell death.…”

Section: +supporting

confidence: 70%

“…36 ), human uveal melanoma (OCM-1) (ref. 39 ), histiocytic lymphoma (U 937), myeloid leukemia (ML-1a) (ref. 40 ), human erythroleukemia (K 562, JM1) (ref.…”

Section: Effects On Programmed Cell Deathmentioning

confidence: 99%

“…43 ), human uveal melanoma (OCM-1) (ref. 39 ), human neuroblastoma (SH-SY5Y), and colon carcinoma (HCT116) 43 cell lines and neonatal rat cardiac myocytes 44 .…”

Section: Effects On Programmed Cell Deathmentioning

confidence: 99%

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Effects of Sanguinarine and Chelerythrine on the Cell Cycle and Apoptosis

Malíková

Zdařilová²,

Hlobílková³

2006

BIOMED PAP

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Section: +supporting

confidence: 70%

“…36 ), human uveal melanoma (OCM-1) (ref. 39 ), histiocytic lymphoma (U 937), myeloid leukemia (ML-1a) (ref. 40 ), human erythroleukemia (K 562, JM1) (ref.…”

Section: Effects On Programmed Cell Deathmentioning

confidence: 99%

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Effects of Sanguinarine and Chelerythrine on the Cell Cycle and Apoptosis

Malíková

Zdařilová²,

Hlobílková³

2006

BIOMED PAP

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“…Sanguinarine-induced apoptosis has been detected in human epidermoid carcinoma (A431) [8] , human prostate cancer (LNCaP, DU-145, PC-3) [22,23] , and breast cancer (MCF-7) cell lines [7] , human endocervical (HeLa) [24] , human melanoma (M4Beu), human colon adenocarcinoma (DLD-1), lung non-small cell carcinoma (A549) [7] , human uveal melanoma (OCM-1) [25] , histiocytic lymphoma (U937), myeloid leukemia (ML-1a) [6] , human erythroleukemia (K562, JM1) cell lines [6,[26][27][28] , immortalized human keratinocytes (HaCaT) [26] and human primary fibroblasts [7,26] . In addition to this information, we found that sanguinarine did not cause ototoxic effect on cochlear cells, while cisplatin caused 20% necrosis on cochlear cells.…”

Section: Discussionmentioning

confidence: 99%

Apoptotic Effects of Sanguinarine on the Organ of Corti 1 Cells: Comparison with Cisplatin

Çeçen¹,

Erçetin²,

Kırkım³

et al. 2015

Int Adv Otol

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OBJECTIVE:Sanguinarine is an alkaloid obtained from the root of Sanguinaria canadensis and other plants from the Papaveraceae family and is well known to possess a broad range of biological functions, such as antimicrobial, antifungal, anti-inflammatory, and antineoplastic activities. We aimed to specify the in vitro effect of sanguinarine on the House Ear Institute-Organ of Corti 1 (HEI-OC1) cells and to compare this effect with the ototoxic effect of cisplatin (CDDP). MATERIALS and METHODS:We performed cell proliferation assay for determining the in vitro effect of sanguinarine alone and compared it with the effect of cisplatin. Flow cytometry annexin-V apoptosis detection was performed. RESULTS:We found that sanguinarine and CDDP inhibited the cell growth in a dose-dependant manner in HEI-OC1 cells after 24 h of incubation. In sanguinarine-treated group, apoptosis was 6.6%, necrosis was 26.7%, and the cell viability was 66.7%. Further, in CDDP-treated group, apoptosis was 5.6%, necrosis was 45.4%, and the cell viability was 48.7%. According to the annexin-V apoptosis detection results, we found that sanguinarine caused 3.9% apoptosis and 1.3% necrosis, while CDDP caused 2.9% apoptosis and 20% necrosis on HEI-OC1 cells. CONCLUSION:Our findings suggested that lower doses of sanguinarine are promising antineoplastic agents, which did not indicate any toxic effect on HEI-OC1 cells. Application of these data to clinical practice requires further support by in vivo studies.

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“…그러나 이러한 현상 은 암세포 특이적으로 나타나며, TRAIL이 apoptosis 유도를 통한 항암효능 증대에 대한 phase 2 clinical trial에 적용되고 있지만, 많은 암세포들이 이미 TRAIL에 대한 저항성을 획득 하고 있다는 점에서 TRAIL의 감수성을 탁월하게 높일 수 있 는 물질의 발굴이 시급한 실정이다 [4,31]. [16,20,23,24,26]. 최근 본 연구실의 결과에 의하면, sanguinarine은 교아종(glioblastoma) 세포에서 mitogen activated 생성 의존적 미토콘드리아 활성화를 통하여 일어났음을 알 수 있었고 [7], 인체 유방암세포의 전이 억제는 치밀 결합(tight junction)의 활성 증가와 matrix metalloproteinase 활성 증가 와 연관성이 있었다 [6].…”

Section: 서 론unclassified

Sanguinarine Increases Sensitivity of Human Gastric Adenocarcinoma Cells to TRAIL-mediated Apoptosis by Inducing DR5 Expression and ROS Generation

Lee¹,

Im²,

Choi³

et al. 2014

Journal of Life Science

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Sanguinarine, a benzophenanthridine alkaloid originally derived from the root of Sanguinaria canadensis, has been shown to possess antimicrobial, antioxidant, and anti-cancer properties. Although tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is known to induce apoptosis in cancer cells, but not most normal cells and has shown efficacy in a phase 2 clinical trial, development of resistance to TRAIL by tumor cells is a major roadblock. Our previous study indicated that treatment with TRAIL in combination with subtoxic concentrations of sanguinarine sensitized TRAIL-mediated apoptosis in TRAIL-resistant human gastric carcinoma AGS cells; however, the detailed mechanisms are not fully understood. In this study, we show that sanguinarine sensitizes AGS cells to TRAILmediated apoptosis as detected by MTT assay, agarose gel electrophoresis, chromatin condensation and flow cytometry analysis. Combined treatment with sanguinarine and TRAIL effectively induced expression of death receptor (DR) 5 but did not affect expression of DR4 and mitogen activated protein kinases signaling molecules. Moreover, the combined treatment with sanguinarine and TRAIL increased the generation of reactive oxygen species (ROS); however, N-acetylcysteine, ROS scavenger, significantly recovered growth inhibition induced by the combined treatment. Taken together, our results indicate that sanguinarine can potentiate TRAIL-mediated apoptosis through up-regulation of DR5 expression and ROS generation. 다[16, 20, 23, 24, 26]. 최근 본 연구실의 결과에 의하면, sanguinarine은 교아종(glioblastoma) 세포에서 mitogen activated

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Apoptotic response of uveal melanoma cells upon treatment with chelidonine, sanguinarine and chelerythrine

Cited by 94 publications

References 23 publications

Effects of Sanguinarine and Chelerythrine on the Cell Cycle and Apoptosis

Effects of Sanguinarine and Chelerythrine on the Cell Cycle and Apoptosis

Apoptotic Effects of Sanguinarine on the Organ of Corti 1 Cells: Comparison with Cisplatin

Sanguinarine Increases Sensitivity of Human Gastric Adenocarcinoma Cells to TRAIL-mediated Apoptosis by Inducing DR5 Expression and ROS Generation

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