Apoptotic properties of the type 1 interferon induced family of human mitochondrial membrane ISG12 proteins

Gytz, Heidi; Hansen, Mariann Fagernæs; Skovbjerg, Signe; Kristensen, Anders C. M.; Hørlyck, Sofie; Jensen, Mette Brehm; Fredborg, Marlene; Markert, Lotte D’Andrea; McMillan, Nigel A.J.; Christensen, Erik; Martensen, Pia M.

doi:10.1111/boc.201600034

Cited by 53 publications

(55 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we found that ISG12a was localized to the mitochondria and nuclear envelope in liver cancer cells, although a most recent study has reported that ISG12a and several of its variants are only localized to the mitochondria 11. In this study, we found that ISG12a was localized to the mitochondria and nuclear envelope in liver cancer cells, although a most recent study has reported that ISG12a and several of its variants are only localized to the mitochondria 11.…”

mentioning

confidence: 50%

“…Unlike other TNF superfamily members, TRAIL was also recognized as an apoptosisrelated ISG. 11,12 ISG12a localizes to the mitochondria, nuclear membrane and endoplasmic reticulum in different cell lines. 6,7 Some studies have reported that ISGs can regulate TRAIL-induced apoptosis.…”

mentioning

confidence: 99%

“…5 IFN-α induces apoptosis in myeloma cells and in solid tumours through induction of TRAIL. 11,12,14,15 The different subcellular distributions of ISG12a suggest that it may have diverse biological functions. Knock-down of Xaf1 in glioblastoma cell lines led to the complete loss of IFN-β-mediated TRAIL sensitivity, thereby indicating that Xaf1 was a crucial ISG and played key roles in TRAIL-induced cell death.…”

mentioning

confidence: 99%

“…6,7 Some studies have reported that ISGs can regulate TRAIL-induced apoptosis. 11 When localized to the inner nuclear membrane, ISG12a interacts with NR4A1 and inhibits its transcription activity. 9 The FAM14 family member G1P3 is another ISG that antagonized TRAIL-induced apoptosis through the inhibition of intrinsic apoptotic pathway.…”

mentioning

confidence: 99%

See 3 more Smart Citations

ISG12a and its interaction partner NR4A1 are involved in TRAIL‐induced apoptosis in hepatoma cells

Liu

Chen

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

Tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) can induce apoptosis in cancer cells while sparing normal cells, thereby leading to the development of TRAIL receptor agonists for cancer treatment. However, these agonist‐based therapeutics exhibit little clinical benefits due to the lack of biomarkers to predict whether patients are responsive to the treatment, as well as determine the resistance of cancer cells to TRAIL‐based agonists. Our previous study has demonstrated that ISG12a enhances TRAIL‐induced apoptosis and might serve as a biomarker to predict the TRAIL response. The downstream mechanism by which ISG12a augments TRAIL‐induced apoptosis remains to be elucidated. In this study, we found that ISG12a was localized in the mitochondria and nucleus and augmented TRAIL‐induced apoptosis through intrinsic apoptotic pathway. In addition, ISG12a interacted with NR4A1 and promoted its nuclear‐to‐cytoplasm translocation. Upon translocate to cytoplasm, NR4A1 targeted mitochondria and induced Bcl2 conformational change, thereby exposing its BH3 domain. Moreover, TRAIL treatment can induce NR4A1 expression through the activation of NF‐κB in TRAIL‐resistant Huh7 hepatoma cells. Knockdown of NR4A1 could overcome TRAIL resistance. However, in TRAIL‐sensitive LH86 liver cancer cells, TRAIL activated the Jun N‐terminal kinases signalling pathway. Overall, these results showed that both ISG12a and its interaction partner NR4A1 are involved in TRAIL‐mediated apoptosis in hepatoma cells.

show abstract

mentioning

confidence: 50%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

ISG12a and its interaction partner NR4A1 are involved in TRAIL‐induced apoptosis in hepatoma cells

Liu

Chen

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…IFI27 as a single gene biomarker can distinguish IFV from bacterial infection (Tang et al, 2017). Mechanistically, IFI27 may be of considerable interest since this gene product is a mitochondrial protein thought to be involved in apoptosis (Gytz et al, 2017; Rosebeck and Leaman, 2008). Thus, IFI27 might mediate some of the type I IFN-induced bystander attrition affects observed in previous studies (Bahl et al, 2006; McNally et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Genomic Circuitry Underlying Immunological Response to Pediatric Acute Respiratory Infection

et al. 2018

View full text Add to dashboard Cite

SUMMARY Acute respiratory tract viral infections (ARTIs) cause significant morbidity and mortality. CD8 T cells are fundamental to host responses, but transcriptional alterations underlying anti-viral mechanisms and links to clinical characteristics remain unclear. CD8 T cell transcriptional circuitry in acutely ill pediatric patients with influenza-like illness was distinct for different viral pathogens. Although changes included expected upregulation of interferon-stimulated genes (ISGs), transcriptional downregulation was prominent upon exposure to innate immune signals in early IFV infection. Network analysis linked changes to severity of infection, asthma, sex, and age. An influenza pediatric signature (IPS) distinguished acute influenza from other ARTIs and outperformed other influenza prediction gene lists. The IPS allowed a deeper investigation of the connection between transcriptional alterations and clinical characteristics of acute illness, including age-based differences in circuits connecting the STAT1/2 pathway to ISGs. A CD8 T cell-focused systems immunology approach in pediatrics identified age-based alterations in ARTI host response pathways.

show abstract

Identification of seven tumor‐educated platelets RNAs for cancer diagnosis

Yuan

Cheng

et al. 2021

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background Tumor‐educated platelets (TEPs) may enable blood‐based cancer diagnosis. This study aimed to identify diagnostic TEPs genes involved in carcinogenesis. Materials and Methods The TEPs differentially expressed genes (DEGs) between healthy samples and early/advanced cancer samples were obtained using bioinformatics. Gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were used to identify the pathways and functional annotation of TEPs DEGs. Protein‐protein interaction of these TEPs DEGs was analyzed based on the STRING database and visualized by Cytoscape software. The correlation analysis and diagnostic analysis were performed to evaluate the diagnostic value of TEPs mRNAs expression for early/advanced cancers. Quantitative real‐time PCR (qRT‐PCR) was applied to validate the role of DEGs in cancers. Results TEPs mRNAs were mostly involved in protein binding, extracellular matrix, and cellular protein metabolic process. RSL24D1 was negatively correlated to early‐stage cancers compared to healthy controls and may be potentially used for early cancer diagnosis. In addition, HPSE, IFI27, LGALS3BP, CRYM, HBD, COL6A3, LAMB2, and IFITM3 showed an upward trend in the expression from early to advanced cancer stages. Moreover, ARL2, FCGR2A, and KLHDC8B were positively associated with advanced, metastatic cancers compared to healthy controls. Among the 12 selected DEGs, the expression of 7 DEGs, including RSL24D1, IFI27, CRYM, HBD, IFITM3, FCGR2A, and KLHDC8B, were verified by the qRT‐PCR method. Conclusion This study suggests that the 7‐gene TEPs liquid‐biopsy biomarkers may be used for cancer diagnosis and monitoring.

show abstract

Apoptotic properties of the type 1 interferon induced family of human mitochondrial membrane ISG12 proteins

Cited by 53 publications

References 57 publications

ISG12a and its interaction partner NR4A1 are involved in TRAIL‐induced apoptosis in hepatoma cells

ISG12a and its interaction partner NR4A1 are involved in TRAIL‐induced apoptosis in hepatoma cells

Genomic Circuitry Underlying Immunological Response to Pediatric Acute Respiratory Infection

Identification of seven tumor‐educated platelets RNAs for cancer diagnosis

Contact Info

Product

Resources

About