Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung&amp;#39;s disease

Du, Chong; Xie, Hua; Zang, Rujin; Zheng, Shen; Li, Hongxing; Chen, Pingfa; Xu, Xiaoqun; Xia, Yankai; Tang, Weibing

doi:10.2147/ijn.s114838

Cited by 23 publications

(14 citation statements)

References 39 publications

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“…For example, lncRNA BACE1-AS is upregulated in Alzheimer's disease (AD) brain tissue and it stabilizes BACE1 mRNA and promotes BACE1 protein expression and Aβ42 formation [23]. In Hirschsprung's disease (HSCR), apoptotic neurons can inhibit apoptosis in non-apoptotic cells by secreting extracellular bodies containing high levels of HN12-lncRNA [24]. In chronic compressive injury (CCI), lncRNA MRAK009713 is a major regulator of neuropathic pain in rats and is significantly increased in CCI rats with enhanced pain behavior [25].…”

Section: Discussionmentioning

confidence: 99%

Dexmedetomidine had neuroprotective effects on hippocampal neuronal cells via targeting lncRNA SHNG16 mediated microRNA-10b-5p/BDNF axis

et al. 2020

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Dexmedetomidine (DEX), a highly selective alpha2 adrenergic receptor agonist, is a commonly used anesthetic drug in surgical procedures. Previous studies have indicated that DEX exerts neuroprotective effects while the detailed mechanism has not been fully elucidated. Here, we aim to study the role of lncRNA SHNG16 in DEX-induced brain protection and its underlying molecular mechanism. The rats underwent middle cerebral artery occlusion (MCAO) surgery and oxygen-glucose deprivation (OGD)-treated HT22 hippocampal neurons were treated with DEX, respectively. CCK8 was used to evaluate cell viability. sh-SHNG16 as well as miR-10b-5p mimics were transfected into hippocampal neurons to further explore the bio-function of SNHG16 and miR-10b-5p in vitro. Furthermore, the interactions between SHNG16 and miR-10b-5p, miR-10b-5p and BDNF gene were confirmed by dual-luciferase report assay. Our data revealed that DEX attenuated neurological damage of the MCAO rats and also increased the cell viability of the neurons significantly. Besides, expression of SHNG16 and BDNF were both downregulated while miR-10b-5p was upregulated in MCAO brain tissues or OGD treated neurons. DEX inhibited miR-10b-5p expression but increased SHNG16 and BDNF levels with a dosage effect. After transfection with sh-SHNG16 or miR-10b-5p mimics, the expression of BDNF protein was downregulated, accompanied with decreased neuron viability. Dual-luciferase assay showed that SHNG16 targeted on miR-10b-5p, which also could bind directly to the 3′-UTR sites of BDNF and negatively regulate its expression. In conclusion, DEX exerts neuroprotective in ischemic stroke via improving neuron damage, the underlying mechanism may be upregulating SHNG16 and BDNF via sponging miR-10b-5p.

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Section: Discussionmentioning

confidence: 99%

Dexmedetomidine had neuroprotective effects on hippocampal neuronal cells via targeting lncRNA SHNG16 mediated microRNA-10b-5p/BDNF axis

et al. 2020

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“…Additionally, the roles of lncRNA transfer may also be implicated in protection against disease as well as in normal physiology of the body. EVs secreted from apoptotic neurons contain high levels of HN12-lncRNA that can be delivered to non-apoptotic cells and can inhibit cell apoptosis in Hirschsprung’s disease (HSCR) by maintaining the function of mitochondria, ATP production and the release of cytochrome C [ 82 ]. This indicates the roles of EV-mediated lncRNA transport in intercellular communication for protecting HSCR development.…”

Section: Ev-mediated Long Non-coding Rna Transport: a Novel Sourcementioning

confidence: 99%

Vesiculated Long Non-Coding RNAs: Offshore Packages Deciphering Trans-Regulation between Cells, Cancer Progression and Resistance to Therapies

Fatima

Nawaz

2017

ncRNA

123

130

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Extracellular vesicles (EVs) are nanosized vesicles secreted from virtually all cell types and are thought to transport proteins, lipids and nucleic acids including non-coding RNAs (ncRNAs) between cells. Since, ncRNAs are central to transcriptional regulation during developmental processes; eukaryotes might have evolved novel means of post-transcriptional regulation by trans-locating ncRNAs between cells. EV-mediated transportation of regulatory elements provides a novel source of trans-regulation between cells. In the last decade, studies were mainly focused on microRNAs; however, functions of long ncRNA (lncRNA) have been much less studied. Here, we review the regulatory roles of EV-linked ncRNAs, placing a particular focus on lncRNAs, how they can foster dictated patterns of trans-regulation in recipient cells. This refers to envisaging novel mechanisms of epigenetic regulation, cellular reprogramming and genomic instability elicited in recipient cells, ultimately permitting the generation of cancer initiating cell phenotypes, senescence and resistance to chemotherapies. Conversely, such trans-regulation may introduce RNA interference in recipient cancer cells causing the suppression of oncogenes and anti-apoptotic proteins; thus favoring tumor inhibition. Collectively, understanding these mechanisms could be of great value to EV-based RNA therapeutics achieved through gene manipulation within cancer cells, whereas the ncRNA content of EVs from cancer patients could serve as non-invasive source of diagnostic biomarkers and prognostic indicators in response to therapies.

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“…A genome-wide association study on Huntington`s disease and copy number variation studies in classic heterotaxy and schizophrenia have identified variants in humaninlike isoform genes [21][22][23]. Humanin isoforms have also been studied as potential biomarkers for Alzheimer`s disease-like dementia and Hirschsprung's disease, suggesting their wider role in physiological processes [24,25]. Research on cancer subtypes, Dicer-binding genes, genomic mosaicism, extracellular RNA profiles, chromosomal rearrangements and huntingtin interactions has provided insight into networks in which humanin-like isoforms might be of importance [26][27][28][29][30][31].…”

Section: Bmc Research Notesmentioning

confidence: 99%

Genetic variants in humanin nuclear isoform gene regions show no association with coronary artery disease

et al. 2019

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Objective: Coronary artery disease contributes to noncommunicable disease deaths worldwide. In order to make preventive methods more accurate, we need to know more about the development and progress of this pathology, including the genetic aspects. Humanin is a small peptide known for its cytoprotective and anti-apoptotic properties. Our study looked for genomic associations between humanin-like nuclear isoform genes and coronary artery disease using CARDIoGRAMplusC4D Consortium data. Results:Lookup from meta-analysis datasets gave single nucleotide polymorphisms in all 13 humanin-like nuclear isoform genes with the lowest P value for rs6151662 from the MTRNR2L2 gene including the 50 kb flanking region in both directions (P-value = 0.0037). Within the gene region alone the top variant was rs78083998 from the MTRNR2L13 region (meta-analysis P-value = 0.042). None of the found associations were statistically significant after correction for multiple testing. Lookup for expression trait loci in these gene regions gave no statistically significant variants.

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Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease

Cited by 23 publications

References 39 publications

Dexmedetomidine had neuroprotective effects on hippocampal neuronal cells via targeting lncRNA SHNG16 mediated microRNA-10b-5p/BDNF axis

Dexmedetomidine had neuroprotective effects on hippocampal neuronal cells via targeting lncRNA SHNG16 mediated microRNA-10b-5p/BDNF axis

Vesiculated Long Non-Coding RNAs: Offshore Packages Deciphering Trans-Regulation between Cells, Cancer Progression and Resistance to Therapies

Genetic variants in humanin nuclear isoform gene regions show no association with coronary artery disease

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