Apoptotic Effect of Saccharomyces cerevisiae on Human Colon Cancer SW480 Cells by Regulation of Akt/NF-ĸB Signaling Pathway

Shamekhi, Sara; Abdolalizadeh, Jalal; Ostadrahimi, Alireza; Mohammadi, Seyed Abolghasem; Lotfi, Hajie; Bonabi, Esat; Zarghami, Nosratollah

doi:10.1007/s12602-019-09528-7

Cited by 43 publications

(34 citation statements)

References 36 publications

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“…The conclusions of the in vivo experiment were consistent with that of the in vitro research, illustrating that heatkilled yeast can reduce the activation of the Akt-NF-κB pathway, which is overactivated in CRC, to promote cell apoptosis. 16 As Akt is also important in the mTOR signaling pathway, 30,31 it suggests that the pro-apoptotic effect of S. cerevisiae may be via the inactivation of the NF-κB and mTOR signaling pathways directly by its surface molecules. All these newly discovered mechanism explorations shed light on the role S. cerevisiae plays in CRC progression and lay a solid foundation for further investigations.…”

Section: S Cerevisiae May Modulate Intestinal Immunity Gut Microbmentioning

confidence: 99%

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Saccharomyces cerevisiae may serve as a probiotic in colorectal cancer by promoting cancer cell apoptosis

Li¹,

Xie²,

Wang³

et al. 2020

J of Digest Diseases

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Objectives: Shotgun metagenomic sequencing of human fecal samples has shown that Saccharomyces cerevisiae (S. cerevisiae) is significantly suppressed in colorectal cancer (CRC) and probably plays an important role in CRC progression. However, these results need to be validated. Here we aimed to confirm the results of highthroughput sequencing and demonstrate the mechanisms mediating the effect of S. cerevisiae on progression from colorectal adenoma (CRA) to CRC. Methods: We used a quantitative polymerase chain reaction (qPCR) assay to examine the relative abundance of S. cerevisiae in 281 fecal samples collected from 106 healthy controls, 108 patients with CRA and 67 with CRC. C57BL/6 and APC Min/+ mouse models and in vitro cell assays were subsequntly used for additional analyses. The mouse models were treated or not treated with broad-spectrum antibiotics and given an S. cerevisiae gavage for 8 weeks. Western blot, 16S rRNA sequencing, qPCR, immunohistochemistry, RNA sequencing, cell counting kit-8 assay, colony formation assay and flow cytometry were performed. Results: S. cerevisiae was 2.68-fold and 3.94-fold less abundant in patients with CRA and CRC, respectively, than in the controls. In vivo experiments showed that S. cerevisiae reduced colorectal tumor progression by promoting epithelial cell apoptosis and modulated gut microbial structure and intestinal immunity. S. cerevisiae downregulated nuclear factor kappa light chain enhancer of activated B cells and the mechanistic target of rapamycin signaling pathways. Cell assays confirmed the proapoptotic effect of S. cerevisiae. Conclusions: S. cerevisiae may play a probiotic role in CRC by promoting cancer cell apoptosis. It can reduce CRC progression by modulating the mucosal microbial structure.

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Section: S Cerevisiae May Modulate Intestinal Immunity Gut Microbmentioning

confidence: 99%

“…8 Several studies have previously suggested that S. cerevisiae may have antiproliferative and pro-apoptotic effects on cancer cells. [13][14][15][16] But the role of S. cerevisiae in CRC remains unclear. Further studies are required to elucidate its role in the development of CRC.…”

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confidence: 99%

Saccharomyces cerevisiae may serve as a probiotic in colorectal cancer by promoting cancer cell apoptosis

Li¹,

Xie²,

Wang³

et al. 2020

J of Digest Diseases

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“…Glinka, et al reported a result consistent with our study that the abundance of Saccharomyces genus was increased in gastrointestinal malignancies [36] . Heat-killed form of Saccharomyces cerevisiae acted as a probiotic and caused apoptosis in colon cancer cells [37] . Such reports with regard to Humicola in cancer are rare.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbes and Microbial Metabolites in Colorectal Cancer Complicated with Different Serum Albumin Levels

Han

Wei

et al. 2020

Preprint

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Background Patients with colorectal cancer (CRC) are at risk of malnutrition. Gut microbes and microbial metabolites are involved in the initiation and development of CRC. Purpose To investigate serum protein levels in CRC patients and explore the role of gut microbes and microbial metabolites in CRCs complicated with different serum albumin levels. Methods Overall, 398 CRC patients and same number of healthy volunteers in Huzhou Central Hospital from January 2016 to December 2018 were recruited to compare serum protein levels. The serological indicators were detected by Abbott Automatic Biochemical Analyzer(HCHL-YQ-SH-01). A total of 30 and 56 stool samples from CRC patients were used to detect intestinal microbes and microbial metabolites, respectively. Bacterial 16S V3-V4 and fungal ITS ribosomal DNA genes were sequenced and gas chromatography/mass spectrometry (GC/MS) was performed to detect microbial metabolites. Results Some serum protein-related indicators in the CRC group were lower than those in the control group (p༜0.05). The total protein and albumin levels in colon cancer patients were lower than those in rectal cancer patients (p༜0.05). The higher abundance of Sutterella is correlated with lower serum albumin level in CRCs. There were statistically significant differences in the abundance of fungi including Agaricomycetes, Simplicillium, Sclerotiniaceae, and Exophiala among patients with different serum albumin levels. Multiple gut bacteria and fungi are closely related to serum albumin levels.We found some characteristic microbial metabolites in CRCs complicated with different serum protein levels. Conclusions The different serum albumin levels were associated with the gut microbes and microbial metabolites in CRCs. It may provide novel ideas for basic research and clinical application.

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“…Colon cancer is one of the common malignant digestive tract tumors and the leading cause of cancer-related death worldwide. Its incidence is high in western countries as well as Iran ranked third among men and fourth in women [14,30]. Surgery, chemotherapy, and radiation therapy are among the main therapeutic modalities for the treatment of colon cancer, however, such treatments are frequently prohibited due to their numerous adverse side effects [3,6].…”

Section: Discussionmentioning

confidence: 99%

“…Further, the development of new anticancer treatment modalities based on probiotics with less adverse effects is urgently needed [1]. Probiotics are known as safe microorganisms, which are thought to have low cytotoxicity, hence, applying probiotic-derived molecules as anticancer agents seem to have fewer adverse effects than the current approaches for colon cancer treatment [1,30]. The beneficial and antitumor effects of probiotics and their derivatives have been reported previously [2,6,8,9,11,13,[15][16][17].…”

Section: Discussionmentioning

confidence: 99%

Prophylactic role of Lactobacillus Paracasei exopolysaccharides on colon cancer cells through apoptosis not ferroptosis

Saadat

Gargari

Shahabi

et al. 2020

Preprint

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Nowadays despite conventional methods in colon cancer treatment, targeting vital molecular pathways and induction of various forms of cell death by safe probiotic components like exopolysaccharides (EPSs) are of great importance and are considered as potential therapeutic agents. This study aimed to investigate the inhibitory effect of the EPS of L. paracasei on different colon cancer cell lines (SW-480, HT-29, and HCT-116). For this purpose, several cellular and molecular experiments including MTS assay, DAPI staining, Annexin V/PI assay, quantitative real-time PCR (qPCR) and some important ferroptosis-related assays were performed. Based on the findings, L. paracasei EPS can induce apoptosis confirmed by all apoptosis-related assays and could not act through ferroptosis pathways. L. paracasei EPS could hinder the Akt1, mTOR, and Jak-1 mRNAs, and induces apoptosis through down-regulation of the anti-apoptotic gene (Bcl-2), up-regulation of pro-apoptotic genes (BAX, caspase-3, 8). The exploited EPS of an indigenous probiotic strain with anticancer potential with low/insignificant cytotoxicity to normal cells is proposed for future applications in molecular-targeted therapy of colon cancer treatment. Furthermore, in vivo and clinical trials should be performed to evaluate the applicability of this component besides conventional methods to increase the survival rate of colon cancer patients.

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Apoptotic Effect of Saccharomyces cerevisiae on Human Colon Cancer SW480 Cells by Regulation of Akt/NF-ĸB Signaling Pathway

Cited by 43 publications

References 36 publications

Saccharomyces cerevisiae may serve as a probiotic in colorectal cancer by promoting cancer cell apoptosis

Saccharomyces cerevisiae may serve as a probiotic in colorectal cancer by promoting cancer cell apoptosis

Gut Microbes and Microbial Metabolites in Colorectal Cancer Complicated with Different Serum Albumin Levels

Prophylactic role of Lactobacillus Paracasei exopolysaccharides on colon cancer cells through apoptosis not ferroptosis

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