Apoptotic Bax Gene Expression in Glioblastoma Cells Exposed to Estradiol valerate

Abstract

Cancers may be influenced by sex steroids in vivo and in vitro. This research aims to determine the effects of estradiol valerate on Bax gene expression level in glioblastoma (A172) cells in cell culture. A172 cells were exposed to cytotoxic dose (0.1 mg/ml ) of estradiol valerate solution. Real time PCR was used to measure Bax gene expression level. Our results indicated that exposure to 0.1 mg/ml of estradiol valerate led to significant increase in viability compared to control cells (P<0.05). Our findings indicated that cytotoxic dose of estradiol have apoptotic effects on brain tumor (A172) cells in cell culture.