Apoptosis within bovine follicular cells and its effect on oocyte development during in vitro maturation

Zeuner, Ann; Müller, Karin; Reguszynski, Karen; Jewgenow, Katarina

doi:10.1016/s0093-691x(02)01190-1

Cited by 65 publications

(51 citation statements)

References 27 publications

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“…Both the present and the previous studies in cattle (Blondin & Sirard 1995, de Wit et al 2000, Zeuner et al 2003 indicate a high correlation between follicle quality or GCs apoptosis and distribution of COCs with different morphology. Since, only grade A and B oocytes were used in this study, that oocytes from early atretic follicles showed a higher, while those from late atretic follicles a lower developmental potential suggested a high correlation between COCs morphology and oocyte competence.…”

Section: Discussionsupporting

confidence: 82%

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Han¹,

Lan²,

Wu³

et al. 2006

Reproduction

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Interactive effects of granulosa cell apoptosis, follicle size, cumulus-oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system Zheng-Bin Han, Guo-Cheng Lan, Yan-Guang Wu, Dong Han, Wei-Guo Feng, Jun-Zuo Wang and Jing-He Tan AbstractUsing a well-in-drop (WID) oocyte/embryo culture system that allows identification of follicular origin, we have investigated the effects of granulosa cells (GCs) apoptosis, follicle size, cumulus-oocyte complexes (COCs) morphology, and cumulus expansion on the developmental competence of goat oocytes matured and cultured individually following parthenogenetic activation. The WID system supported oocyte maturation and embryo development to a level similar to the conventional group system. The majority of goat oocytes acquired competence for development up to the 8-16 cell stage in follicles larger than 2 mm, but did not gain the ability to form morula/blastocyst (M/Bs) until follicles larger than 3 mm in diameter. The extent of atresia affected M/Bs formation. This effect varied according to the follicle size. Cumulus expansion increased with follicle size and decreased with increasing incidence of GCs apoptosis. Oocyte developmental potential was also correlated with cumulus expansion. Regardless of the degree of follicle atresia, 73-84% of the floating cells in the follicular fluid (FF) underwent apoptosis. Correlation between floating cell density in FF and oocyte developmental potency suggests the possibility to use the floating cell density as a simple and non-invasive marker for oocyte quality. It is concluded that the developmental potential of an oocyte is determined by multifactor interactions, and multiple factors must be considered together to accurately predict the quality of an oocyte. Reproduction (2006) 132 749-758Introduction A number of studies have shown that in vitro development of pre-implantation stage mammalian embryos is better when cultured in groups (group culture) than cultured singly in microdrops (microdrop individual culture, MIC) (Gardner et al. 1994, Kato & Tsunoda 1994, Keefer et al. 1994, Moessner & Dodson 1995. However, apart from the large-scale production of embryos for experimental purposes, most commercial and human embryo culture systems require culturing individually or in small groups. The reason for this need is the small number of oocytes available (ovum pick-up (OPU) technology, single slaughtered valuable oocyte donors and human IVF) or the required identification after culture. Besides, sometimes zona-free eggs must be cultured in isolation from each other to prevent conglomeration. Therefore, the establishment of an individual culture system that is as efficient as group culture has become of prime importance. Furthermore, although there are quite a few reports on individual culture of embryos, studies on individual culture of oocytes during maturation are few and with conflicting results (Carolan et al. 1996, Hagemann et al. 1998, Jewgenow et al. 19...

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Section: Discussionsupporting

confidence: 82%

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Han¹,

Lan²,

Wu³

et al. 2006

Reproduction

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“…In our study, this role could be attributed ZEN -the loosening of tight junctions and the expansion of intercellular spaces was observed in group EI, and these changes were particularly pronounced in group EII. Other researchers have not observed direct correlations between follicle cell and follicular wall morphology and oocyte quality (Zeuner et al 2003). According to these authors, a certain number of ovarian follicles undergo apoptotic changes, and the apoptotic signal is transmitted from follicle cells to the oocyte only when a critical activity level is exceeded, e.g., when ATP levels drop as a result of mitochondrial damage (Moreira et al 2011).…”

Section: Discussionmentioning

confidence: 99%

The effect of experimental low zearalenone intoxication on ovarian follicles in pre-pubertal bitches

Gajęcka¹

2013

Polish Journal of Veterinary Sciences

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Companion animals, including bitches, may be exposed to zearalenone (ZEN) toxins that are often present in feed, and ZEN intoxication may lead to ovarian dysfunction. This study involved evaluation of the degree of ZEN-induced hypo stimulation of ovary by determination of proliferative and apoptotic indices and description of the ultra-structural organization of ovarian follicles in pre-pubertal bitches subjected to experimental, long-term exposure to low-dose ZEN mycotoxicosis. The experiment involved 30 clinically healthy, immature Beagle bitches aged approximately 70 days with initial average body weight of 8 kg, randomly divided into three groups of 10 animals each: two experimental groups (EI and EII) and a control group (C). Over a period of 42 days, ZEN was administered per os to EI animals at a dose of 50 μg/kg BW, and to EII bitches at a dose of 75 μg/kg BW. Control group animals were fed placebo containing no ZEN for 42 days. Analytical samples of the mycotoxin were administered daily in gelatin capsules before morning feeding. All the bitches were subjected to ovario-hysterectomy at the end of the experiment. Proliferation index (PCNA method) and apoptotic index (TUNEL test) values were determined by immune-histological analyses. The median for apoptotic index was determined at 13.45 for group EI, 17.84 for group EII, and 8.59 for group C. The median for proliferation index was determined at 35.25 for group EII, 42.44 for group EI, and 70.60 for group C. The results of ultra-structural examinations of the ovaries revealed that experimental, ZEN-induced hyperestrogenism enhanced apoptosis and lowered the proliferative ability of follicular cells what contributed to organelle destruction in pre-pubertal bitches. The changes observed were particularly advanced in EII animals, which were administered a higher dose of ZEN.

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“…One of the indications of these techniques is therapeutic purposes, for example in ovarian hyperstimulation syndrome that cycle cancellation is done [1]. Other use is learning the basic biological actions going on during oocyte maturation, fertilization and early embryonic development.…”

Section: Introductionmentioning

confidence: 99%

Influence of Insulin-Like Growth Factor-I on Maturation and Fertilization Rate of Immature Oocyte and Embryo Development in NMRI Mouse with TCM199 and α -MEM Medium

Toori

Mosavi

Nikseresht

et al. 2014

JCDR

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Apoptosis within bovine follicular cells and its effect on oocyte development during in vitro maturation

Cited by 65 publications

References 27 publications

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

The effect of experimental low zearalenone intoxication on ovarian follicles in pre-pubertal bitches

Influence of Insulin-Like Growth Factor-I on Maturation and Fertilization Rate of Immature Oocyte and Embryo Development in NMRI Mouse with TCM199 and α -MEM Medium

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