Apoptosis: why and how does it occur in biology?

Ulukaya, Engin; Acılan, Ceyda; Yılmaz, Yusuf

doi:10.1002/cbf.1774

Cited by 189 publications

(129 citation statements)

References 197 publications

Supporting

Mentioning

121

Contrasting

Unclassified

Order By: Relevance

“…Apoptosis is a programmed mode of cell death that is involved in a wide variety of physiological and pathological processes (Krampe and Al-Rubeai 2010;Pereira and Amarante-Mendes 2011;Ulukaya et al 2011). Detection of apoptosis is of great importance for many areas of biological research, for understanding the pathological conditions and for the development of new drugs.…”

Section: Introductionmentioning

confidence: 99%

Beyond annexin V: fluorescence response of cellular membranes to apoptosis

2012

View full text Add to dashboard Cite

Dramatic changes in the structure of cell membranes on apoptosis allow easy, sensitive and non-destructive analysis of this process with the application of fluorescence methods. The strong plasma membrane asymmetry is present in living cells, and its loss on apoptosis is commonly detected with the probes interacting strongly and specifically with phosphatidylserine (PS). This phospholipid becomes exposed to the cell surface, and the application of annexin V labeled with fluorescent dye is presently the most popular tool for its detection. Several methods have been suggested recently that offer important advantages over annexin V assay with the ability to study apoptosis by spectroscopy of cell suspensions, flow cytometry and confocal or twophoton microscopy. The PS exposure marks the integrated changes in the outer leaflet of cell membrane that involve electrostatic potential and hydration, and the attempts are being made to provide direct probing of these changes. This review describes the basic mechanisms underlying the loss of membrane asymmetry during apoptosis and discusses, in comparison with the annexin V-binding assay, the novel fluorescence techniques of detecting apoptosis on cellular membrane level. In more detail we describe the detection method based on smart fluorescent dye F2N12S incorporated into outer leaflet of cell membrane and reporting on apoptotic cell transformation by easily detectable change of the spectral distribution of fluorescent emission. It can be adapted to any assay format.

show abstract

Section: Introductionmentioning

confidence: 99%

Beyond annexin V: fluorescence response of cellular membranes to apoptosis

2012

View full text Add to dashboard Cite

show abstract

“…Las células expuestas a ellos exhiben cambios asociados con la apoptosis, tales como la ruptura de la doble hélice de ADN, asociados con la activación de la caspasa 3/7, y la condensación y fragmentación de la cromatina nuclear, eventos que se observan incluso en ausencia del gen p53, el Bax o la caspasa 3 (35). Este hallazgo es prometedor, pues la mayoría de las células cancerígenas que son resistentes a la muerte celular se asocian con mutaciones en el p53, en la caspasa 3 o en miembros de la familia de las Bcl2 (36).…”

Section: Discussionunclassified

Cytotoxic effect of palladium (II) inclusion compounds in beta-cyclodextrin

et al. 2016

View full text Add to dashboard Cite

Introducción. Las tiosemicarbazonas y sus complejos de paladio (II) poseen actividad antineoplásica con pocos efectos secundarios, por lo cual se las considera como una nueva alternativa terapéutica. Sin embargo, existen diferencias en los rangos de la concentración inhibitoria media (CI50) asociada a la divergencia estructural y la solubilidad de los complejos, así como a la sensibilidad de los blancos celulares. La inclusión de fármacos en la beta-ciclodextrina con fines terapéuticos ha mejorado su solubilidad y estabilidad, pero los efectos de su combinación con los complejos de paladio (II) y las tiosemicarbazonas no se han comprobado aún.Objetivo. Estudiar el efecto citotóxico de los complejos de paladio en la beta-ciclodextrina.Materiales y métodos. La actividad citotóxica de los complejos de paladio en la beta-ciclodextrina se evaluó en la línea celular de cáncer de mama (MCF-7), empleando el método de la sulforodamina B.Resultados. Los ligandos MePhPzTSC y Ph2PzTSC, sus complejos de paladio (II) libres e incluidos en la beta-ciclodextrina y el cisplatino mostraron actividad citotóxica en la línea celular MCF-7; sin embargo, la citotoxicidad fue mayor con la inclusión en la beta-ciclodextrina ([Pd(MePhPzTSC)2]•ß-CD y [Pd(Ph2PzTSC)2]•ß-CD). La concentración inhibitoria media (CI50) para estos complejos se obtuvo en concentraciones de 0,14 y 0,49 μM, y con dosis hasta cinco veces inferiores comparadas con las concentraciones de los ligandos libres (1,4 y 2,9 μM), de los complejos de paladio (II) libres (0,57 y 1,24 μM) y del cisplatino (6,87 μM).Conclusiones. El uso de la beta-ciclodextrina mejoró significativamente la actividad citotóxica de las tiosemicarbazonas y sus complejos de paladio (II), lo cual probablemente está asociado al incremento de la solubilidad y biodisponibilidad del compuesto, estrategia que se puede sugerir para el diseño de futuros fármacos antineoplásicos.

show abstract

“…Apoptosis, which is also known as programmed cell death, may be the most popular word in cancer, aging and development research (Ulukaya et al 2011). Currently, apoptosis is believed to play an important role in early weaning piglet weaned stress syndrome (Zhu et al , 2012.…”

Section: Discussionmentioning

confidence: 99%

“…Researchers usually classify apoptotic pathways into extrinsic and intrinsic according to whether the major factors that induce apoptosis are extracellular or intracellular (Ulukaya et al 2011;Zhu et al 2013).…”

Section: Discussionmentioning

confidence: 99%

X/XO or H2O2 induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets

et al. 2014

View full text Add to dashboard Cite

We previously demonstrated that intestinal epithelial cell apoptosis in weaned piglets is much more serious than that observed in sucking piglets and is related to oxidative stress during weaning. It is difficult to study the apoptosis mechanisms only using in vivo methods because of the limit of existing research technology. An in vitro cellular system is required for piglet intestinal epithelial cell apoptosis research. In this study, a non-tumorigenic epithelial cell line, IPEC-J2 cells, was employed as a cell model. Hydrogen peroxide and xanthine/xanthine oxidase (X/ XO) were both used and compared for apoptosis modeling. The concentrations of hydrogen peroxide and XO were selected and verified using cell viability analysis, the comet assay and flow cytometry. Intracellular ROS were measured using fluorescent probes. Additionally, the expression levels of the apoptosis-related genes Fas, Bcl-2, P53, Caspase 3, Caspase 8, and Caspase 9 were analyzed using quantitative RT-PCR. The results indicated the optimal modeling method is a final concentration of 0.5 mM H 2 O 2 incubated with IPEC-J2 cells for 1 h at 37°C in 5 % CO 2 for hydrogen peroxide-induced apoptosis modeling, and a final concentration of 250 lM X/50 U/L XO incubated with IPEC-J2 cells for 6 h at 37°C in 5 % CO 2 for X/XO-induced apoptosis modeling. For the apoptotic pathway, the X/XO modeling method is more similar to 21 days weaning piglets. Therefore, we suggest that X/XO modeling with IPEC-J2 cells be used as an in vitro cell culture model for weaning piglet intestinal epithelial cell apoptosis.

show abstract

Apoptosis: why and how does it occur in biology?

Cited by 189 publications

References 197 publications

Beyond annexin V: fluorescence response of cellular membranes to apoptosis

Beyond annexin V: fluorescence response of cellular membranes to apoptosis

Cytotoxic effect of palladium (II) inclusion compounds in beta-cyclodextrin

X/XO or H2O2 induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets

Contact Info

Product

Resources

About