Apoptosis Versus Necrosis During Cold Storage and Rewarming of Human Renal Proximal Tubular Cells1

Salahudeen, Abdulla K.; Joshi, Manish K.; Jenkins, John K.

doi:10.1097/00007890-200109150-00010

Cited by 81 publications

(67 citation statements)

References 19 publications

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“…Although cold ischemic injury, as in warm ischemic injury, is considered to be due to massive cell necrosis, several recent studies suggest that the apoptotic form of cell death does occur, but only after the reperfusion of transplanted organs (6, 46, 62, 65). Our current view is that cold ischemia causes cell necrosis, but during reperfusion a significant proportion of sublethally injured cells undergo apoptosis (65). Data from Burns et al (6) in cold-stored human kidney transplants and our own in vitro study in renal tubular cells (64) are consistent with this view.…”

Section: Mode Of Cell Death During Cold Storage and Reperfusion: Targsupporting

confidence: 51%

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Cold ischemic injury of transplanted kidneys: new insights from experimental studies

Salahudeen¹

2004

American Journal of Physiology-Renal Physiology

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143

128

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Kidney transplantation is the preferred and definitive treatment for end-stage renal disease (ESRD), and kidneys from deceased donors are a major source for it. These kidneys are routinely cold stored to prolong viability, which, however, when prolonged can cause injury, resulting in reduced graft function and survival. Recent experimental studies have identified the release of iron and free radicals, activation of calpain, and formation of F2-isoprostanes as important components of cold ischemic injury, as are the swelling of mitochondria and activation of mitochondrial apoptotic pathways. Moreover, studies have also suggested that fortifying the storage solution with deferoxamine or preconditioning the donor kidneys with hemeoxygenase-1 may prove viable clinical strategies to limit cold ischemic injury. This review will summarize these and other new experimental data that have implications for reducing cold ischemic transplant injury, a step necessary to improve deceased-donor allograft survival.

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Section: Mode Of Cell Death During Cold Storage and Reperfusion: Targsupporting

confidence: 51%

“…1) (65). This prompted us to study the role of the mitochondrial apoptotic pathway in the setting of cold ischemic renal injury (62,65).…”

Section: Mode Of Cell Death During Cold Storage and Reperfusion: Targmentioning

confidence: 99%

Cold ischemic injury of transplanted kidneys: new insights from experimental studies

Salahudeen¹

2004

American Journal of Physiology-Renal Physiology

Self Cite

143

128

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“…Mechanism of cell death during cold storage could be apoptotic, necrotic or mixed type as described before in various organ cooling. [15][16][17][18] both islets and exocrine. The first is collagenase enzyme, which enhances destruction of normal tissue architecture to facilitates isolation of islets from surrounding exocrine cells; the second is the rising temperature of pancreas, which is needed to activate the enzyme; and the third is the mechanical dissociation, which enables purification of islets for transplantation later as previously described in reference 6.…”

Section: Discussionmentioning

confidence: 99%

Augmented damage of islets by impaired exocrine acinar cells undergoing apoptosis that is possibly converted to necrosis during isolation

Elgendy

Okitsu²,

Kimura³

et al. 2011

Islets

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“…Este autor ha presentado unos magníficos trabajos donde se aportan datos tanto moleculares como microscópicos 2,3,[22][23][24] .…”

Section: Nuevos Conceptos En Isquemia Fría Y Daño Renalunclassified

“…Es decir, la isquemia se relaciona con destrucción celular directa por necrosis. La revascularización o reperfusión con pérdida celular por apoptosis 25 .…”

Section: Nuevos Conceptos En Isquemia Fría Y Daño Renalunclassified

Innovaciones en la compresión de los efectos de la isquemia fría en el injerto renal

García

Martínez

et al. 2005

Actas Urol Esp

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RESUMEN INNOVACIONES EN LA COMPRESIÓN DE LOS EFECTOS DE LA ISQUEMIA FRÍA EN EL INJERTO RENALLa isquemia fría es el mejor método actual para preservar el riñón para trasplante. Sin embargo condiciona, en primer lugar, un proceso de necrosis celular directo. En segundo lugar, durante el período de hipotermia se desarrolla un proceso de lesión mitocondrial que hace que la célula entre en un estado de apoptosis latente. Esta apoptosis se completa definitivamente en esta población celular en el momento de la revascularización. Las soluciones de preservación actualmente disponibles no son perfectas, y no pueden evitar este proceso. La adición de ciertas sustancias en la solución de UW ha demostrado experimentalmente (Desferrioxamina) una reducción de las lesiones mitocondriales producidas durante la fase de hipotermia. La utilización de perfusión de riñón aislado podría, comparativamente con la hipotermia simple, reducir la aparición de disfunción inicial del injerto hasta en un 20%, lo que tendría consecuencias importantes tanto funcionales como económicas. ABSTRACT RECENT AVANCES IN THE COMPREHENSION OF THE EFFECTS OF COLD ISCHEMIA IN KIDNEY GRAFTCold ischemia is the best known method to preserve kidneys for transplant. However, it produces several detrimental effects. First, cellular necrosis. Secondarily, during the hypothermic period a mithocondrial injury process develops which makes the cell entering a pre-apoptotic state. This apoptosis occurs definitively in the reperfusion. Preservation solutions currently available are not perfect and are not able to avoid cold-related cell injuries. The adition of certain substances to UW solution (desferrioxamine) has shown experimentally a reduction in mitochondrial cold-related lesions. Isolated hypothermic kidney perfusion reduces initial graft dysfunction about 20% in comparison to hypothermic storage. This fact relates to important either economical as functional consequences.Keywords: Renal Transplant. Cold Ischemia. Renal Preservation Innovaciones en la compresión de los efectos de la isquemia fría en el injerto renal

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Apoptosis Versus Necrosis During Cold Storage and Rewarming of Human Renal Proximal Tubular Cells1

Cited by 81 publications

References 19 publications

Cold ischemic injury of transplanted kidneys: new insights from experimental studies

Cold ischemic injury of transplanted kidneys: new insights from experimental studies

Augmented damage of islets by impaired exocrine acinar cells undergoing apoptosis that is possibly converted to necrosis during isolation

Innovaciones en la compresión de los efectos de la isquemia fría en el injerto renal

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