Apoptosis, ROS and Calcium Signaling in Human Spermatozoa: Relationship to Infertility

Bejarano, Ignacio; Espino, Javier Díez; Paredes, Sergio D.; Ortiz, Águeda; Lozano, Graciela M.; Pariente, José A.; Rodríguez, Ana B.

doi:10.5772/32617

Cited by 14 publications

(12 citation statements)

References 132 publications

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“…When a cell fails to maintain cellular homeostasis utilizing the total available antioxidant capacity oxidative stress is generated that expedites the process of apoptosis [ 25 ]. To protect cells from ROS mediated apoptosis, the parasite must carefully control the level of ROS by upregulating antioxidant defense.…”

Section: Introductionmentioning

confidence: 99%

Deprivation of L-Arginine Induces Oxidative Stress Mediated Apoptosis in Leishmania donovani Promastigotes: Contribution of the Polyamine Pathway

et al. 2016

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The growth and survival of intracellular parasites depends on the availability of extracellular nutrients. Deprivation of nutrients viz glucose or amino acid alters redox balance in mammalian cells as well as some lower organisms. To further understand the relationship, the mechanistic role of L-arginine in regulation of redox mediated survival of Leishmania donovani promastigotes was investigated. L-arginine deprivation from the culture medium was found to inhibit cell growth, reduce proliferation and increase L-arginine uptake. Relative expression of enzymes, involved in L-arginine metabolism, which leads to polyamine and trypanothione biosynthesis, were downregulated causing decreased production of polyamines in L-arginine deprived parasites and cell death. The resultant increase in reactive oxygen species (ROS), due to L-arginine deprivation, correlated with increased NADP+/NADPH ratio, decreased superoxide dismutase (SOD) level, increased lipid peroxidation and reduced thiol content. A deficiency of L-arginine triggered phosphatidyl serine externalization, a change in mitochondrial membrane potential, release of intracellular calcium and cytochrome-c. This finally led to DNA damage in Leishmania promastigotes. In summary, the growth and survival of Leishmania depends on the availability of extracellular L-arginine. In its absence the parasite undergoes ROS mediated, caspase-independent apoptosis-like cell death. Therefore, L-arginine metabolism pathway could be a probable target for controlling the growth of Leishmania parasites and disease pathogenesis.

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Section: Introductionmentioning

confidence: 99%

Deprivation of L-Arginine Induces Oxidative Stress Mediated Apoptosis in Leishmania donovani Promastigotes: Contribution of the Polyamine Pathway

et al. 2016

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“…Recently, it has been reported that apoptosis plays a major role in male infertility-related diseases [24,33], and an altered apoptosis process has been found to be closely associated with male infertility and with sperm quality such as motility, viability, and sperm defects [34]. In the course of apoptosis, Bcl-2 antiapoptotic and Bax proapoptotic proteins provide a signaling pathway that helps maintain balance in a cell [17,18].…”

Section: Discussionmentioning

confidence: 99%

Insulinlike growth factor I improves yak (Bos grunniens) spermatozoa motility and the oocyte cleavage rate by modulating the expression of Bax and Bcl-2

et al. 2015

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“…This is a very interesting finding, since the homeostasis of [Ca 2+ ] i is one of the key parameters that drives sperm function. It is involved in the control of several function (from motility 50 to cytoskeleton assembly 51,52 , from lipid remodelling 53 to apoptosis 54 ) and, on the other side, it is controlled by a myriad of factors either at intracellular and extracellular level. Nevertheless, recent reports have described a potential negative effect of GO exposure of sperm cells 55 , somatic stem cells 56 as well as bacteria 57 .…”

Section: Discussionmentioning

confidence: 99%

Graphene Oxide increases mammalian spermatozoa fertilizing ability by extracting cholesterol from their membranes and promoting capacitation

Bernabò

Machado-Simoes

Valbonetti

et al. 2019

Sci Rep

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Graphene Oxide (GO) is a widely used biomaterial with an amazing variety of applications in biology and medicine. Recently, we reported the ability of GO to improve the in vitro fertilization (IVF) outcomes in swine, a validated animal model with a high predictive value for human fertility. For that reason, here we characterized the mechanisms involved in this positive interaction by adopting an experimental approach combining biological methods (confocal microscopy analysis on single cell, flow cytometry on cell populations and co-incubation with epithelial oviductal cells), physical-chemical techniques (Differential Scanning Calorimetry and Thermogravimetric Analysis), and chemical methods (mass spectrometry and lipid measurement). As a result, we propose a model in which GO is able to extract cholesterol from the spermatozoa membrane without causing any detrimental effect. In this way, the cholesterol extraction promotes a change in membrane chemical-physical properties that could positively affect male gamete function, modulating sperm signalling function and increasing in this way the fertilizing potential, without losing the ability to physiologically interact with the female environment. In conclusion, these data seem to suggest new intriguing possibilities in engineering sperm membrane for improving assisted reproduction technologies outcomes, even in human medicine.

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Apoptosis, ROS and Calcium Signaling in Human Spermatozoa: Relationship to Infertility

Cited by 14 publications

References 132 publications

Deprivation of L-Arginine Induces Oxidative Stress Mediated Apoptosis in Leishmania donovani Promastigotes: Contribution of the Polyamine Pathway

Deprivation of L-Arginine Induces Oxidative Stress Mediated Apoptosis in Leishmania donovani Promastigotes: Contribution of the Polyamine Pathway

Insulinlike growth factor I improves yak (Bos grunniens) spermatozoa motility and the oocyte cleavage rate by modulating the expression of Bax and Bcl-2

Graphene Oxide increases mammalian spermatozoa fertilizing ability by extracting cholesterol from their membranes and promoting capacitation

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